Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FZ025_RS04610 Genome accession   NZ_CP043476
Coordinates   1003144..1004880 (-) Length   578 a.a.
NCBI ID   WP_046978045.1    Uniprot ID   -
Organism   Xanthomonas hyacinthi strain CFBP 1156     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 998144..1009880
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FZ025_RS04590 (FZ025_04640) - 999274..1000887 (+) 1614 WP_104557685.1 HAMP domain-containing sensor histidine kinase -
  FZ025_RS04595 (FZ025_04645) pilR 1000963..1002351 (+) 1389 WP_104557687.1 sigma-54 dependent transcriptional regulator Regulator
  FZ025_RS04600 (FZ025_04650) - 1002524..1002820 (+) 297 WP_046978042.1 type II toxin-antitoxin system RelE/ParE family toxin -
  FZ025_RS04605 (FZ025_04655) - 1002823..1003116 (+) 294 WP_046978041.1 addiction module antidote protein -
  FZ025_RS04610 (FZ025_04660) pilB 1003144..1004880 (-) 1737 WP_046978045.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FZ025_RS04615 (FZ025_04665) - 1005088..1005378 (+) 291 WP_046978040.1 nucleotidyltransferase family protein -
  FZ025_RS04620 (FZ025_04670) - 1005375..1005746 (+) 372 WP_046978039.1 DUF86 domain-containing protein -
  FZ025_RS04625 (FZ025_04675) - 1005882..1006577 (+) 696 WP_104557689.1 transposase -
  FZ025_RS04630 (FZ025_04680) - 1006977..1007414 (-) 438 WP_046978037.1 pilin -
  FZ025_RS04635 (FZ025_04685) pilC 1007801..1009060 (+) 1260 WP_046978036.1 type II secretion system F family protein Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62808.99 Da        Isoelectric Point: 5.7896

>NTDB_id=384415 FZ025_RS04610 WP_046978045.1 1003144..1004880(-) (pilB) [Xanthomonas hyacinthi strain CFBP 1156]
MNALTSANLVGITGIARRLVQDGALEETVARTAMAHAAEAKIPLPQWFSEKKLVTAAQLAAANAVEFGMPLLDVSVFDAN
QSAIKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWQASNDAIGNSLG
GDDEGMDNLEVGAGDEDMGGGGDTGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKSVAKAP
IKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKIVLRILDGSAARLGIDKLGYEPEQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDDTRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAVALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPTQLP
NNALLAEGFSEAEVAAGIQLYEAVGCDECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIADAAQKIGVKDLRQSALI
KARAGITSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=384415 FZ025_RS04610 WP_046978045.1 1003144..1004880(-) (pilB) [Xanthomonas hyacinthi strain CFBP 1156]
ATGAACGCTCTGACATCCGCCAATCTCGTCGGCATCACCGGCATCGCGCGCCGCCTGGTCCAGGATGGCGCCTTGGAAGA
GACGGTCGCGCGGACCGCGATGGCGCATGCCGCCGAGGCCAAGATCCCGCTGCCGCAGTGGTTCTCCGAGAAGAAGCTGG
TCACCGCGGCGCAGCTGGCGGCCGCCAATGCGGTCGAGTTCGGCATGCCGCTGCTGGACGTGTCGGTGTTCGATGCCAAC
CAGAGCGCGATCAAGCTGGTCAGCGAGGAGTTGCTGCAGAAGCATCAGGTGTTGCCGCTGTTCAAGCGCGGTAACCGGCT
GTTTGTGGGGGTCAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAATCTGACGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGTTGGAGCAGTGGCAGGCGAGCAACGATGCGATCGGCAACAGCCTGGGC
GGCGACGACGAAGGCATGGACAACCTCGAGGTCGGTGCCGGCGACGAGGACATGGGCGGCGGCGGCGATACCGGCGTCGA
TGCCAAGGGCGACGATACGCCGGTGGTCAAGTTCGTGAACAAGATGCTGGTCGATGCGATCCGCCGCGGCGCTTCGGACA
TCCATTTCGAGCCGTACGAGGACGATTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGAGCGTGGCCAAGGCGCCG
ATCAAGCTCAATCAGCGCATCGCCGCGCGGCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGA
TGGGCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACGCTGTTCGGCGAGA
AGATCGTGCTGCGTATCCTCGACGGCAGTGCGGCCAGGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAGCAGCAAAAG
CTCTTTCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCT
GTACACCGCGCTGGGCATCCTCAACGACGATACCCGCAACATCTCCACCGCCGAAGATCCGGTGGAAATCCGCCTGCCCG
GGGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACGTTCGCCGTGGCGCTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTTGGCGAAATCCGCGACCTGGAGACGGCGGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCA
CCAGTTCGGTGACCTTGGTGATCGCGCAGCGCCTGGCGCGGCGGCTGTGCAACAACTGCAAGCGCCCGACCCAGCTACCG
AACAATGCGCTGTTGGCGGAGGGATTCAGCGAGGCCGAGGTCGCCGCCGGCATCCAGTTGTACGAGGCGGTGGGCTGCGA
CGAGTGCACCGAGGGCTACAAGGGCCGCACCGGCATCTATCAGGTGATGCCGATGAACGACGAGATCGCGGCCATCGTAT
TGCAGGGCGGCAATGCGATGGACATCGCCGATGCGGCGCAGAAGATCGGGGTCAAGGACTTGCGCCAGTCGGCGCTGATC
AAGGCGCGCGCCGGCATCACCAGCCTGGCCGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.124

100

0.545
  pilB Acinetobacter baylyi ADP1

54.31

100

0.545
  pilB Legionella pneumophila strain ERS1305867

52.724

98.443

0.519
  pilB Vibrio cholerae strain A1552

47.782

100

0.484
  pilF Neisseria gonorrhoeae MS11

48.768

98.27

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.82

97.924

0.458
  pilB Vibrio campbellii strain DS40M4

46.573

98.443

0.458
  pilF Thermus thermophilus HB27

40

90.83

0.363

Multiple sequence alignment