Detailed information    

insolico Bioinformatically predicted

Overview

Name   comQ/pilQ   Type   Machinery gene
Locus tag   HF909_RS02435 Genome accession   NZ_CP051169
Coordinates   503256..505394 (+) Length   712 a.a.
NCBI ID   WP_280926350.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RUN2474     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498256..510394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HF909_RS02415 (HF909_02440) pilM 500333..501403 (+) 1071 WP_020747313.1 type IV pilus biogenesis protein PilM -
  HF909_RS02420 (HF909_02445) - 501417..502049 (+) 633 WP_193027423.1 PilN domain-containing protein -
  HF909_RS02425 (HF909_02450) - 502052..502711 (+) 660 WP_193027424.1 type 4a pilus biogenesis protein PilO -
  HF909_RS02430 (HF909_02455) - 502708..503253 (+) 546 WP_193027425.1 pilus assembly protein PilP -
  HF909_RS02435 (HF909_02460) comQ/pilQ 503256..505394 (+) 2139 WP_280926350.1 temperature dependent type IV pilus secretin PilQ Machinery gene
  HF909_RS02440 (HF909_02465) - 505537..506109 (+) 573 WP_193028418.1 shikimate kinase -
  HF909_RS02445 (HF909_02470) aroB 506106..507212 (+) 1107 WP_193027427.1 3-dehydroquinate synthase -
  HF909_RS02450 (HF909_02475) - 507232..508395 (+) 1164 WP_193027428.1 deoxyguanosinetriphosphate triphosphohydrolase -
  HF909_RS02455 (HF909_02480) - 508545..509219 (-) 675 WP_193027429.1 OmpW/AlkL family protein -
  HF909_RS02460 (HF909_02485) - 509488..510213 (+) 726 WP_193027430.1 transposase -

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76335.42 Da        Isoelectric Point: 9.8259

>NTDB_id=382927 HF909_RS02435 WP_280926350.1 503256..505394(+) (comQ/pilQ) [Ralstonia pseudosolanacearum strain RUN2474]
MRRVKGGLAQAVRVARGGAVAWLSLCMLMVGGQALAQQAITAPASNTVERVEQASAGESTVVTVTLKDAPAQKPVEFSTQ
QPARIAIDFFGTAFAQGRANYQYGGKLLRAASVVQIGDRTRVVLDLARQSQYKSEIRGNQYVLTLGAAPTASATPVPTFA
APAPTAGVERPSVRNIDFRRGEELAGRVVVDLSTSNSAINIAQQGQNLVVDFTGATLPQSLRRRFDVSDFGTPVQAMRAT
DNGTGARLVIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERMSLNFQNIDIRSLLQVFADFTNLNIVTSD
SVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRNELATKEKAELESQQQVTELEPLRSQVFRLNYQRADDV
RNMLLGTGAAAGGGAAASRILSKRGSLTADARTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEADDTFSRNLG
AKLGFAAKTNGAGYGNTYSNVVSPVTKDATWDNSSAVSLPANGINGVNAASVAVSLFNAGAGRFLALELSALEADGRGKI
ISSPRVVTADNIKALIEQGTEVPYQQATSSGATSVSFKKANLKLEVTPKITPDGNVFLDVDVNKDSLGTQTTNGFAINTK
HVQTQVLVENGGTVVIGGIYTQNERTDVNKVPLLGDIPVLGNLFKSTAKTNDRTELLVFLTPRVLSDQLSLK

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=382927 HF909_RS02435 WP_280926350.1 503256..505394(+) (comQ/pilQ) [Ralstonia pseudosolanacearum strain RUN2474]
ATGCGTCGCGTGAAGGGCGGCCTGGCGCAGGCCGTTCGAGTGGCCCGGGGGGGCGCAGTGGCATGGCTTTCGCTGTGTAT
GCTGATGGTGGGCGGACAGGCGTTGGCCCAGCAGGCAATCACGGCGCCGGCAAGCAATACCGTCGAACGCGTGGAACAAG
CCTCGGCAGGCGAATCCACCGTGGTGACGGTGACGCTGAAGGACGCGCCTGCCCAGAAGCCGGTGGAATTCAGCACGCAG
CAGCCTGCGCGCATCGCCATCGACTTCTTCGGCACGGCATTCGCCCAAGGGCGTGCCAACTATCAATATGGCGGCAAGCT
GCTGCGCGCGGCCAGCGTGGTGCAGATCGGTGACCGCACCCGCGTTGTGCTGGATCTGGCGCGCCAGTCGCAGTACAAGT
CCGAGATCCGGGGCAACCAGTACGTGCTGACGCTGGGCGCCGCGCCGACGGCGTCTGCTACCCCGGTGCCGACCTTCGCC
GCACCCGCGCCGACGGCCGGTGTGGAGCGGCCGTCCGTGCGCAACATCGACTTCCGCCGTGGCGAAGAGCTGGCTGGCCG
TGTGGTGGTCGACCTGTCGACGTCCAACTCCGCGATCAACATCGCCCAGCAGGGGCAAAACCTGGTGGTCGATTTCACCG
GCGCCACGCTGCCGCAATCGCTGCGTCGCCGCTTTGACGTGAGCGACTTCGGCACGCCGGTGCAGGCCATGCGCGCCACC
GACAACGGCACGGGGGCCCGCCTGGTCATCGAGCCGCGCGGCAACTGGCAGTACAGCTCGTACCAGACCGACACGCAGTT
CGTGGTGGAAGTCCGCCCGACCAAGGAGGACCCGAACAAGCTGATCTCCGGTCCGGGCTATCGCGGGGAGCGCATGTCGC
TGAACTTCCAGAACATCGACATCCGTTCGCTGCTGCAGGTCTTTGCCGACTTCACCAACCTGAACATCGTCACCAGCGAC
AGCGTGACGGGGACCCTGTCGCTGCGTCTGAAGGACGTGCCGTGGGATCAGGCGCTGCAGATCGTGCTGGATTCCAAGGG
CTTGGCCTCGCGCCGAAATGGCAACGTGCTGTGGGTGGCGCCGCGCAACGAACTGGCGACCAAGGAGAAGGCCGAGCTGG
AGTCGCAGCAACAGGTGACCGAGCTGGAGCCGCTGCGCAGCCAGGTGTTCCGTCTGAATTACCAGCGTGCTGATGACGTG
CGCAACATGCTGCTGGGTACCGGAGCAGCGGCGGGCGGTGGTGCTGCGGCGTCGCGTATCTTGTCCAAGCGCGGTTCGCT
GACGGCGGATGCCCGCACCAACCAGCTGTTTGTCTCGGACATTCCGAGCAAGCTGGAGGAGGTGCAGGCTTTCCTGCTGA
AGATCGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCTGATGACACGTTTAGTCGCAACCTAGGT
GCAAAGCTGGGCTTCGCGGCCAAGACCAATGGTGCCGGATATGGCAACACCTACAGCAACGTGGTGTCGCCGGTCACGAA
AGACGCCACTTGGGACAACAGCTCGGCCGTCAGCCTGCCCGCCAACGGCATCAACGGCGTGAACGCTGCCAGCGTTGCGG
TGAGCCTGTTCAACGCCGGTGCCGGCCGTTTCCTGGCGCTCGAACTGTCGGCGCTGGAAGCCGACGGCCGCGGCAAGATC
ATCTCCAGCCCGCGTGTGGTCACCGCTGACAACATCAAGGCGCTGATCGAGCAGGGTACCGAAGTGCCTTACCAGCAGGC
GACCTCGTCGGGCGCGACGTCGGTGTCGTTCAAAAAGGCCAACCTGAAATTGGAGGTGACGCCGAAGATTACGCCGGACG
GCAACGTGTTCCTGGACGTGGACGTCAACAAGGACAGCCTGGGTACGCAGACCACCAACGGCTTCGCCATCAACACCAAG
CACGTGCAGACCCAGGTGCTGGTCGAGAATGGCGGTACGGTTGTGATTGGCGGTATCTACACGCAGAACGAACGGACCGA
TGTGAACAAGGTCCCGCTGCTGGGTGACATCCCGGTGCTGGGCAACCTGTTCAAGAGCACGGCCAAGACCAACGACCGGA
CGGAACTGCTGGTGTTCCTGACGCCGCGCGTGCTGTCCGACCAGCTGTCGCTGAAGTAA

Domains

Predicted by InterProScan.

AMIN domain (AMIN)

(195-270)

AMIN domain (AMIN)

(63-143)

Bacterial type II and III secretion system protein (Secretin)

(551-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(388-461)

Secretin and TonB N terminus short domain (STN)

(316-361)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comQ/pilQ Acinetobacter baylyi ADP1

37.382

100

0.389
  pilQ Pseudomonas aeruginosa PAK

41.168

93.82

0.386
  pilQ Acinetobacter baumannii D1279779

36.552

100

0.372
  pilQ Acinetobacter baumannii strain A118

36.552

100

0.372
  pilQ Neisseria gonorrhoeae strain FA1090

36.314

100

0.371
  pilQ Legionella pneumophila strain ERS1305867

39.269

92.275

0.362
  pilQ Legionella pneumophila strain Lp02

39.269

92.275

0.362