Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FXF61_RS00875 Genome accession   NZ_CP043320
Coordinates   183187..185274 (+) Length   695 a.a.
NCBI ID   WP_151183494.1    Uniprot ID   A0A5J6Q5I4
Organism   Pseudomonas sp. C27(2019)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 178187..190274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXF61_RS00855 (FXF61_00855) pilM 180352..181416 (+) 1065 WP_151183490.1 pilus assembly protein PilM Machinery gene
  FXF61_RS00860 (FXF61_00860) - 181416..181991 (+) 576 WP_151183491.1 PilN domain-containing protein -
  FXF61_RS00865 (FXF61_00865) pilO 181988..182611 (+) 624 WP_151183492.1 type 4a pilus biogenesis protein PilO -
  FXF61_RS00870 (FXF61_00870) - 182608..183135 (+) 528 WP_151183493.1 pilus assembly protein PilP -
  FXF61_RS00875 (FXF61_00875) pilQ 183187..185274 (+) 2088 WP_151183494.1 type IV pilus secretin PilQ family protein Machinery gene
  FXF61_RS00880 (FXF61_00880) aroK 185274..185798 (+) 525 WP_151183495.1 shikimate kinase AroK -
  FXF61_RS00885 (FXF61_00885) aroB 185907..187004 (+) 1098 WP_151183496.1 3-dehydroquinate synthase -
  FXF61_RS00890 (FXF61_00890) - 187020..188582 (+) 1563 WP_151183497.1 SPOR domain-containing protein -
  FXF61_RS00895 (FXF61_00895) - 188591..189421 (+) 831 WP_151183498.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
  FXF61_RS00900 (FXF61_00900) - 189418..190068 (-) 651 WP_151183499.1 TetR family transcriptional regulator -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75582.99 Da        Isoelectric Point: 4.9784

>NTDB_id=382849 FXF61_RS00875 WP_151183494.1 183187..185274(+) (pilQ) [Pseudomonas sp. C27(2019)]
MKTKLSRIGMTLLAAAFSPALLAANLQTLDVAALPGDKVELKLAFDEPITAPRGYTIEQPARIALDLPGVKNKLGTKNLE
LGAGNARSVSVVEAQDRTRLIINMSRLSPYSTRAEGKHLYIVVGDNVSASAPVVNTPVVKPYAAQQRGISNIDFQRGEQG
EGNVVIELSDPNNTPDIQDQGGKIRLTFAQSVLPEALRVRLDVKDFATPVQFVSASESNGKVAIVIEPSGVYDYLAYQTD
DKLTISVKPVTQDEIEKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQALDLV
LKTKGLDKRKIGNVLLVAPADEIAARERQEMEAQKQIAELAPLRRELIQVNYAKASQIAALFQSVTSDQSDVRGSVTVDD
RTNSIIAYQTQDKLDELRRVVAQLDIPVRQVMIEARIVEANVDYSKSLGVRWGGALMSNTGKWNAFGKDGNIGIKDNDET
NCGPFAGNCTLPTINNSPVPFVDMGVQNATAGLGIGFITNHAILDLQLSAMEKTGNGEVVSQPKVVTSDKETAKILKGAE
IPYQEASSSGSTSTSFKEAALSLEVTPQITPDNRIIMDVKVTKDEPDFANALNGVPPIKKNEVNAKVLVTDGETIVIGGV
FSNTQTKSVDKVPFLGDLPFIGRIFRRDYIQDQKSELLVFITPRIMNNQAISLNN

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=382849 FXF61_RS00875 WP_151183494.1 183187..185274(+) (pilQ) [Pseudomonas sp. C27(2019)]
ATGAAAACAAAACTATCTCGTATCGGTATGACTTTGCTGGCAGCGGCATTTTCACCAGCATTGCTAGCCGCCAACCTGCA
AACATTAGATGTAGCAGCATTGCCTGGCGACAAGGTGGAACTTAAATTAGCCTTTGATGAACCGATCACTGCACCGCGCG
GCTACACCATTGAGCAGCCCGCTCGAATTGCTTTGGATTTGCCCGGTGTTAAAAATAAATTGGGTACTAAAAATCTAGAG
CTTGGCGCAGGTAATGCACGCAGTGTCAGTGTTGTTGAGGCGCAAGATCGCACACGATTAATTATTAATATGAGTCGTCT
GTCGCCCTATAGCACGCGTGCAGAAGGTAAGCACTTGTATATTGTGGTGGGCGATAATGTGAGTGCGTCAGCACCTGTTG
TCAACACGCCGGTTGTTAAGCCTTACGCTGCTCAGCAGCGCGGTATCAGTAATATTGATTTTCAGCGTGGTGAGCAAGGT
GAAGGTAATGTTGTTATTGAGCTCAGTGATCCTAACAACACACCTGATATTCAAGATCAAGGTGGCAAGATTCGCCTGAC
ATTTGCTCAGTCAGTATTGCCAGAAGCGTTGCGCGTACGCTTAGATGTTAAAGATTTTGCAACGCCTGTGCAGTTTGTCA
GTGCCAGTGAGTCTAATGGCAAAGTTGCAATCGTCATTGAGCCAAGTGGTGTTTACGATTATTTGGCTTATCAAACTGAT
GATAAGCTAACAATTAGTGTTAAACCTGTCACGCAAGATGAAATAGAAAAACGCAATGCAGAACGCTTTGCTTATACCGG
TGAAAAGCTATCACTAAACTTCCAAGATATTGATGTGCGTTCAGTGTTGCAGTTGATTGCTGACTTCACTGATCTAAACT
TAGTTGCCAGTGATACTGTGCAGGGTAACATCACATTACGCTTACAAAATGTACCTTGGGATCAAGCGCTTGATTTAGTG
TTGAAAACTAAAGGTCTTGATAAGCGTAAAATTGGTAACGTGTTACTCGTAGCGCCTGCAGATGAAATTGCTGCACGTGA
GCGTCAAGAAATGGAGGCGCAGAAGCAAATTGCTGAACTGGCACCTTTGCGTCGTGAGCTGATTCAAGTGAACTATGCAA
AAGCATCACAAATTGCGGCTTTATTCCAATCAGTGACCAGTGATCAAAGTGATGTGCGCGGCTCAGTCACAGTGGATGAT
CGCACCAACAGCATTATTGCTTACCAGACTCAAGATAAGCTTGATGAGTTACGCCGTGTTGTAGCACAGCTCGATATTCC
AGTGCGGCAGGTAATGATTGAAGCGCGTATTGTTGAGGCGAACGTTGATTACAGCAAAAGTTTAGGTGTCCGTTGGGGCG
GTGCTTTGATGAGCAATACGGGCAAATGGAATGCCTTTGGTAAAGATGGAAACATTGGTATTAAAGACAATGATGAGACC
AACTGCGGACCATTTGCAGGTAACTGTACACTGCCAACGATTAATAATAGCCCTGTGCCTTTTGTTGATATGGGCGTACA
GAATGCTACAGCTGGCTTGGGTATTGGTTTTATTACCAATCACGCCATTCTTGATCTGCAGCTTTCTGCAATGGAAAAAA
CTGGTAACGGTGAAGTGGTATCACAGCCCAAGGTTGTGACCTCTGATAAAGAAACAGCGAAAATCCTGAAAGGTGCTGAA
ATTCCATACCAAGAAGCAAGTTCTAGCGGTTCAACCAGTACCTCATTTAAAGAAGCGGCGTTATCGCTGGAAGTGACACC
GCAAATCACGCCTGATAACCGCATCATTATGGATGTTAAGGTCACTAAGGACGAGCCTGACTTTGCCAATGCACTAAACG
GTGTGCCACCGATTAAAAAGAATGAGGTGAATGCCAAAGTATTGGTGACTGATGGTGAAACGATCGTGATTGGTGGTGTG
TTCTCTAATACGCAAACAAAATCAGTGGATAAAGTGCCATTCTTGGGTGACTTACCCTTTATTGGCCGCATTTTCCGCCG
AGATTACATCCAAGACCAAAAGTCTGAACTGCTGGTATTTATTACCCCGCGTATTATGAACAATCAGGCTATTTCACTGA
ATAATTAA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(293-339)

AMIN domain (AMIN)

(38-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(367-430)

Bacterial type II and III secretion system protein (Secretin)

(530-686)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6Q5I4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

75.07

100

0.771
  comQ Acinetobacter baylyi ADP1

41.931

99.856

0.419
  pilQ Acinetobacter baumannii strain A118

41.295

100

0.413
  pilQ Acinetobacter baumannii D1279779

41.295

100

0.413
  pilQ Legionella pneumophila strain ERS1305867

37.741

96.835

0.365
  pilQ Legionella pneumophila strain Lp02

37.593

96.835

0.364

Multiple sequence alignment