Detailed information NTG0382848

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FXF61_RS00855
• Genome accession: NZ_CP043320
• Coordinates: 180352..181416 (+)
• Length: 354 a.a.
• NCBI ID: WP_151183490.1
• Uniprot ID: A0A5J6Q5Q5
• Organism: Pseudomonas sp. C27(2019)
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 175352..186416

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FXF61_RS00840 (FXF61_00840)	-	175487..176239 (+)	753	WP_178087234.1	thermonuclease family protein	-
FXF61_RS00845 (FXF61_00845)	-	176418..177686 (+)	1269	WP_151183488.1	malic enzyme-like NAD(P)-binding protein	-
FXF61_RS00850 (FXF61_00850)	-	177764..180166 (-)	2403	WP_371921520.1	penicillin-binding protein 1A	-
FXF61_RS00855 (FXF61_00855)	pilM	180352..181416 (+)	1065	WP_151183490.1	pilus assembly protein PilM	Machinery gene
FXF61_RS00860 (FXF61_00860)	-	181416..181991 (+)	576	WP_151183491.1	PilN domain-containing protein	-
FXF61_RS00865 (FXF61_00865)	pilO	181988..182611 (+)	624	WP_151183492.1	type 4a pilus biogenesis protein PilO	-
FXF61_RS00870 (FXF61_00870)	-	182608..183135 (+)	528	WP_151183493.1	pilus assembly protein PilP	-
FXF61_RS00875 (FXF61_00875)	pilQ	183187..185274 (+)	2088	WP_151183494.1	type IV pilus secretin PilQ family protein	Machinery gene
FXF61_RS00880 (FXF61_00880)	aroK	185274..185798 (+)	525	WP_151183495.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38047.54 Da
• Isoelectric Point: 4.4059

>NTDB_id=382848 FXF61_RS00855 WP_151183490.1 180352..181416(+) (pilM) [Pseudomonas sp. C27(2019)]
MLGLFKKKANTLLGIDISSTSVKLIELSRSSGRYKVEAYAVEPLPLNAIVEKNIAELDLVGQVISKLLSKARTNIKQAAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGPAPRSNERVNVLLAACRKENVEVREAALAL
AGLSAKVVEIEAYALERAYGLLAEQLSDSEDDLTVAVVDIGATMMTLSVLHNGQTIYTREQIFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYESEVLTPFKEAVVQQVSRSLQFFFAAGQYSDVDYIILAGGTASIVGLDQLVQQKIGTPTLVANP
FANMTLSSKVNAGALASDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=382848 FXF61_RS00855 WP_151183490.1 180352..181416(+) (pilM) [Pseudomonas sp. C27(2019)]
GTGCTAGGGCTCTTCAAAAAGAAAGCGAATACGCTGCTGGGGATCGACATAAGCTCGACTTCAGTTAAGCTCATAGAACT
GAGTCGCTCAAGTGGCCGCTATAAAGTAGAGGCGTACGCTGTTGAGCCGCTCCCACTCAATGCAATCGTAGAGAAAAATA
TTGCGGAACTTGATTTAGTTGGACAGGTGATTTCTAAACTGCTCAGTAAAGCGCGCACTAATATTAAACAAGCGGCAGTT
GCAGTTGCTGGCTCTGCAGTGATTACTAAAACGATTGAAATGGACGCTGGGCTTTCTGATGATGAATTAGAAAATCAGCT
CAAGATTGAAGCGGATCAATACATTCCATACCCTCTTGAAGAGGTTGCAATCGACTTTGAGGTGCAAGGGCCTGCGCCGC
GCAGTAATGAGCGGGTTAATGTATTGCTTGCAGCTTGTCGTAAAGAAAACGTAGAAGTACGGGAAGCGGCATTAGCATTG
GCAGGGTTGAGTGCGAAAGTTGTCGAAATTGAGGCGTATGCTCTAGAGCGTGCTTATGGCTTACTGGCTGAGCAGCTGAG
TGATAGTGAGGATGATCTAACGGTTGCGGTTGTTGATATTGGTGCAACCATGATGACGCTCAGTGTCCTGCATAACGGTC
AAACAATTTATACGCGTGAACAAATTTTTGGTGGCAAACAGCTCACAGAAGAAATTCAGCGTCGCTATGGTTTGTCTGTA
GAAGAGGCGGGTCTGGCTAAAAAGCAAGGTGGCTTGCCAGATGATTATGAGAGCGAAGTATTGACCCCCTTTAAAGAGGC
GGTTGTTCAGCAAGTGTCACGCTCCTTGCAGTTCTTTTTTGCTGCGGGACAGTACAGCGATGTCGATTACATCATCTTAG
CAGGTGGCACAGCTTCGATTGTCGGGCTGGATCAGCTTGTTCAACAAAAAATAGGTACGCCAACTCTTGTGGCTAACCCT
TTTGCCAATATGACGCTAAGCAGCAAGGTCAATGCAGGTGCATTAGCAAGTGATGCGCCTTCTTTGATGATTGCTTGTGG
CTTAGCGATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A5J6Q5Q5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	54.52	100	0.545
comM	Acinetobacter nosocomialis M2	54.52	100	0.545
comM	Acinetobacter baylyi ADP1	50.847	100	0.508
pilM	Legionella pneumophila strain ERS1305867	45.763	100	0.458