Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FYN22_RS12680 Genome accession   NZ_CP043307
Coordinates   2619405..2620526 (+) Length   373 a.a.
NCBI ID   WP_148826640.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain Acsw19     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2614405..2625526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYN22_RS12665 (FYN22_12665) - 2616611..2617345 (-) 735 WP_148826638.1 hypothetical protein -
  FYN22_RS12670 (FYN22_12670) - 2617529..2618218 (-) 690 WP_148826639.1 YggS family pyridoxal phosphate-dependent enzyme -
  FYN22_RS12675 (FYN22_12675) pilT 2618341..2619378 (+) 1038 WP_004694862.1 type IV pilus twitching motility protein PilT Machinery gene
  FYN22_RS12680 (FYN22_12680) pilU 2619405..2620526 (+) 1122 WP_148826640.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FYN22_RS12685 (FYN22_12685) fur 2620591..2621028 (-) 438 WP_086208199.1 ferric iron uptake transcriptional regulator -
  FYN22_RS12690 (FYN22_12690) - 2621141..2621539 (+) 399 WP_004694868.1 outer membrane protein assembly factor BamE -
  FYN22_RS12695 (FYN22_12695) - 2621586..2621909 (-) 324 WP_148826641.1 RnfH family protein -
  FYN22_RS12700 (FYN22_12700) - 2621909..2622991 (-) 1083 WP_148826642.1 hypothetical protein -
  FYN22_RS12705 (FYN22_12705) - 2623134..2623589 (-) 456 WP_005400160.1 bacteriohemerythrin -
  FYN22_RS12710 (FYN22_12710) - 2623864..2624712 (-) 849 WP_148826643.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41876.14 Da        Isoelectric Point: 6.6662

>NTDB_id=382771 FYN22_RS12680 WP_148826640.1 2619405..2620526(+) (pilU) [Acinetobacter johnsonii strain Acsw19]
MDFNDLLNYMIQQKASDLFITADVEPSMKINGQIVPVAKGKLTGDVAGQLLNSIMSDKQRKEFADTRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETQIPTMDELKLPPVLKELAMTKRGIIIFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLKAMVAQQLIPTPDGNSRRAAIEILINSPLISDLIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQITYKDALKHADSPNDLRLQIKLSEEGGDRLLHANANITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=382771 FYN22_RS12680 WP_148826640.1 2619405..2620526(+) (pilU) [Acinetobacter johnsonii strain Acsw19]
ATGGACTTTAACGACTTACTCAATTATATGATTCAACAAAAAGCGTCGGATTTATTTATCACCGCTGATGTTGAACCCTC
CATGAAAATTAACGGTCAAATTGTGCCTGTGGCTAAGGGTAAGCTGACGGGTGATGTCGCGGGGCAACTGCTCAACTCGA
TTATGAGTGACAAGCAGCGTAAAGAATTTGCGGACACACGTGAGTGCAACTTCGCGATTATGAATCGGGATAAAACTGCA
CGTTTTCGTGTCAGTGCCTTTCAACAGCGTGATATGCCAGGTATGGTTTTGCGTCGAATTGAGACGCAAATTCCAACCAT
GGATGAGTTAAAACTCCCGCCTGTACTCAAAGAGTTAGCCATGACCAAACGTGGCATCATTATCTTTGTGGGTGCAACTG
GTACAGGTAAATCGACCTCTTTGGCTTCGATCATTGGTTACCGTAACCAAAACTCGAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTATTCATGAGCATGCAGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACAGACTCTTTTGA
AATCGCGCTGAAAAACACCTTACGTCAGGCACCCGATGTGATCCTGATTGGTGAGATTCGTTCCCGTGAAACCATGGATT
ATGCAATTGCCTTCGCGGAAACAGGTCACTTGGTATTTGCAACACTGCACGCCAACAACGCCAACCAAGCCATCGACCGT
ATTATTCACTTCTTTGAAGCAGACCGTCATAGCCAATTGTTTATGGACTTATCTTTAAATCTTAAAGCGATGGTTGCTCA
GCAGTTGATTCCAACACCTGACGGTAATTCACGTCGTGCTGCCATTGAAATTTTGATTAACTCACCGTTAATTTCCGATT
TGATCCGTAAAGGTGAAATCCACGAAATTAAAGACCTGATGAAGCGTTCTCGTGAACTCGGTATGCAAACCTTTGACCAA
GCCTTGTTTGATTTGTACAAAGCCAAGCAGATTACCTATAAAGATGCGCTGAAACACGCTGACTCACCAAACGACTTACG
TTTACAAATCAAATTATCAGAAGAAGGCGGCGATCGTCTATTGCATGCCAATGCAAATATCACGTTTGATGGACAAGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-250)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

76.72

100

0.777
  pilU Pseudomonas stutzeri DSM 10701

67.655

99.464

0.673
  pilU Vibrio cholerae strain A1552

57.265

94.102

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

41.42

90.617

0.375
  pilT Legionella pneumophila strain ERS1305867

41.42

90.617

0.375
  pilT Pseudomonas aeruginosa PAK

39.306

92.761

0.365

Multiple sequence alignment