Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA/celA/cilE   Type   Machinery gene
Locus tag   GRB95_RS04125 Genome accession   NZ_CP051004
Coordinates   750316..750969 (+) Length   217 a.a.
NCBI ID   WP_000461744.1    Uniprot ID   Q8E0F2
Organism   Streptococcus agalactiae 515     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 745316..755969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GRB95_RS04100 (GRB95_04100) - 745484..746314 (+) 831 WP_000735514.1 MetQ/NlpA family ABC transporter substrate-binding protein -
  GRB95_RS04105 (GRB95_04105) - 746549..748135 (+) 1587 WP_000673088.1 DEAD/DEAH box helicase -
  GRB95_RS04110 (GRB95_04110) - 748320..748586 (-) 267 WP_000598736.1 GIY-YIG nuclease family protein -
  GRB95_RS04115 (GRB95_04115) - 748579..749343 (-) 765 WP_000567423.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  GRB95_RS04120 (GRB95_04120) - 749478..750218 (+) 741 WP_000500220.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  GRB95_RS04125 (GRB95_04125) comEA/celA/cilE 750316..750969 (+) 654 WP_000461744.1 helix-hairpin-helix domain-containing protein Machinery gene
  GRB95_RS04130 (GRB95_04130) comEC/celB 750953..753190 (+) 2238 WP_000939916.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  GRB95_RS04135 (GRB95_04135) - 753316..754125 (+) 810 WP_000153219.1 Cof-type HAD-IIB family hydrolase -
  GRB95_RS04140 (GRB95_04140) - 754136..755080 (+) 945 WP_000200824.1 LacI family DNA-binding transcriptional regulator -

Sequence

Protein


Download         Length: 217 a.a.        Molecular weight: 23713.07 Da        Isoelectric Point: 9.5543

>NTDB_id=382598 GRB95_RS04125 WP_000461744.1 750316..750969(+) (comEA/celA/cilE) [Streptococcus agalactiae 515]
MFEIVLEKIKSHKWETTGIIVGLLLFGILGLNHFGTHHKEDNLNINLEKKVSTITEKKVPMISHVKDKVSNQVTVDVKGA
VNHPGVYSLPSQSRVTDAIKRAGGLSNLADSKSVNLAQKLQDETVIYVAQKGEKITVVEEEKANNIATQGNSKGKINLNK
ADLSSLQTISGVGAKRAQDILDYRDSQGGFKTIDDLKNVSGIGEKTLEKLRQDVTID

Nucleotide


Download         Length: 654 bp        

>NTDB_id=382598 GRB95_RS04125 WP_000461744.1 750316..750969(+) (comEA/celA/cilE) [Streptococcus agalactiae 515]
ATGTTTGAAATAGTATTGGAAAAAATTAAATCGCATAAATGGGAGACAACAGGAATTATTGTAGGACTCTTATTATTTGG
AATTTTGGGTTTAAATCATTTTGGCACTCATCATAAAGAAGACAATTTAAATATCAATTTAGAGAAAAAAGTATCAACTA
TAACAGAAAAAAAGGTACCAATGATTAGTCACGTCAAAGATAAAGTATCTAATCAAGTAACGGTTGACGTAAAAGGGGCA
GTCAATCATCCAGGTGTTTATAGTTTGCCTTCTCAAAGTCGTGTAACAGATGCGATTAAGCGTGCAGGTGGACTAAGCAA
TCTCGCTGATAGTAAATCCGTTAATCTAGCACAGAAATTACAAGATGAGACGGTTATCTATGTTGCTCAAAAAGGTGAAA
AAATAACTGTTGTGGAAGAGGAGAAAGCAAATAATATCGCCACTCAGGGAAATAGTAAGGGGAAAATTAATCTTAATAAA
GCAGATTTATCAAGTCTTCAGACCATATCAGGCGTTGGAGCTAAAAGAGCTCAAGATATTTTAGATTATCGTGATAGTCA
AGGTGGTTTTAAAACTATTGATGACCTCAAAAACGTATCAGGTATTGGTGAGAAAACACTAGAAAAATTACGTCAAGATG
TTACAATTGACTAA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(75-128)

Helix-hairpin-helix motif (HHH_3)

(153-215)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8E0F2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

45

100

0.456
  comEA/celA/cilE Streptococcus pneumoniae Rx1

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae D39

43.694

100

0.447
  comEA/celA/cilE Streptococcus pneumoniae R6

43.694

100

0.447
  comEA/celA/cilE Streptococcus mitis NCTC 12261

43.243

100

0.442
  comEA/celA/cilE Streptococcus mitis SK321

43.636

100

0.442
  comEA Lactococcus lactis subsp. cremoris KW2

39.013

100

0.401
  comEA Streptococcus thermophilus LMD-9

54.545

70.968

0.387