Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FXO11_RS13135 Genome accession   NZ_CP043042
Coordinates   2954027..2955742 (+) Length   571 a.a.
NCBI ID   WP_148863393.1    Uniprot ID   -
Organism   Marinobacter fonticola strain CS412     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2949027..2960742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FXO11_RS13115 - 2949536..2950300 (-) 765 WP_148863389.1 Wzz/FepE/Etk N-terminal domain-containing protein -
  FXO11_RS13120 rfaH 2950550..2951029 (-) 480 WP_148863390.1 transcription/translation regulatory transformer protein RfaH -
  FXO11_RS13125 - 2951414..2952937 (-) 1524 WP_148863391.1 O-antigen ligase -
  FXO11_RS13130 - 2953218..2953664 (-) 447 WP_148863392.1 pilin -
  FXO11_RS13135 pilB 2954027..2955742 (+) 1716 WP_148863393.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FXO11_RS13140 pilC 2955745..2956965 (+) 1221 WP_148863394.1 type II secretion system F family protein Machinery gene
  FXO11_RS13145 pilD 2957053..2957928 (+) 876 WP_148863395.1 A24 family peptidase Machinery gene
  FXO11_RS13150 coaE 2957976..2958602 (+) 627 WP_148863396.1 dephospho-CoA kinase -
  FXO11_RS13155 - 2958680..2959132 (+) 453 WP_148863397.1 HIT family protein -
  FXO11_RS13160 - 2959107..2960177 (+) 1071 WP_227545901.1 hypothetical protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62686.65 Da        Isoelectric Point: 4.7471

>NTDB_id=380290 FXO11_RS13135 WP_148863393.1 2954027..2955742(+) (pilB) [Marinobacter fonticola strain CS412]
MALNNQNVTLTGLARRFVEDGLLDEAAARDAFLQASNNKIPLITYLVQNNLAESRGLAFSAAMEFGVSVLDLSSFAPDML
PEKIVDEKLIRKHNALPLYKRGKRLYIAVSDPTNIQALDEIKFNTGLSTDAVLVDDARLRELIDGYLESSESSMGDLADS
ELGDMEVDVEGADEDQGESAAADIDDAPIVKYVNKILLDAIRGGASDVHFEPYEKVYRVRYRTDGILKEMSKPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDALGYEEEQKEMYLTAL
EQPQGMILVTGPTGSGKTVSLYTGLNILNTQERNISTAEDPAEINLEGINQVNVNTRVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAPETLTRMMNMGVPAFNIATSVSLIIAQRLARRLCSCKESLDAPSDVLLKE
GFTEQQIETGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELANMIMEEASSIQIAKQAQAEGFANLRQSALKKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=380290 FXO11_RS13135 WP_148863393.1 2954027..2955742(+) (pilB) [Marinobacter fonticola strain CS412]
ATGGCGCTCAACAATCAGAATGTGACACTGACAGGACTTGCCCGTCGCTTCGTGGAAGACGGACTGCTGGATGAAGCTGC
TGCAAGGGATGCCTTTCTTCAGGCTTCAAACAACAAAATCCCCCTCATCACTTATCTGGTACAGAACAACCTGGCCGAAA
GCCGTGGATTGGCTTTTTCAGCTGCCATGGAGTTCGGAGTTTCCGTACTAGACCTGTCCTCATTTGCCCCGGATATGCTT
CCGGAGAAGATTGTTGATGAAAAACTCATTCGCAAGCATAACGCACTGCCTTTGTACAAAAGGGGTAAGCGCCTTTATAT
CGCTGTTTCTGACCCCACCAACATCCAGGCCCTGGATGAGATCAAGTTCAATACCGGCCTGAGTACCGACGCGGTCCTCG
TCGACGACGCAAGGCTGCGCGAACTTATCGACGGCTATCTCGAATCCAGCGAATCATCCATGGGTGACCTTGCCGATTCG
GAGCTTGGTGACATGGAAGTGGATGTCGAGGGCGCCGATGAAGATCAAGGTGAAAGCGCGGCGGCCGACATCGATGACGC
GCCTATCGTCAAGTACGTTAACAAGATTCTTCTGGACGCGATTCGCGGCGGCGCCTCGGATGTCCATTTTGAACCTTATG
AAAAGGTCTACCGGGTCCGTTATCGTACCGACGGTATTCTGAAGGAGATGTCCAAGCCTTCGATAAAACTTGCGCCCAAG
ATCTCCGCCCGGGTCAAGATCATGGCTCAATTGGATATTTCTGAGCGCCGCGTCCCTCAGGATGGGCGGATCAAGATGAA
GCTATCCAAAACGAAGGCTATTGATTTTCGGGTCAATACGTTGCCGACACTCTGGGGCGAGAAAATCGTGCTTCGGATTC
TGGACCCCAGCCAAGCCAAACTGGGTATCGATGCGCTTGGCTATGAGGAAGAGCAGAAGGAGATGTACCTGACCGCGCTG
GAACAGCCGCAAGGGATGATCCTTGTGACTGGCCCCACCGGTTCAGGTAAAACAGTTTCTCTTTATACCGGGCTGAATAT
CCTCAATACCCAAGAACGCAACATCTCCACCGCTGAGGACCCAGCGGAAATCAATCTTGAGGGCATCAACCAGGTCAACG
TTAACACCCGGGTGGGCCTTGGCTTCGCCGAAGCATTGCGCGCATTCCTGCGCCAGGATCCTGACGTCATCATGGTCGGC
GAGATCCGGGACCTTGAAACCGCGAACATTGCGATCAAAGCGGCCCAAACCGGTCACCTGGTGCTTTCGACGCTCCACAC
GAACAGCGCCCCGGAAACCTTGACTCGAATGATGAACATGGGGGTGCCTGCCTTCAATATCGCGACCTCGGTGAGTCTAA
TCATTGCCCAGCGTTTGGCCCGTCGGCTTTGCAGCTGTAAGGAGTCTTTAGACGCCCCAAGCGATGTCCTTTTAAAGGAA
GGCTTCACAGAGCAACAAATTGAAACAGGCTTTACTTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGGGGATATAA
AGGCCGCGTCGGTATTTACGAGGTGGTGAAGATTACGGACGAGCTGGCCAACATGATTATGGAAGAAGCCAGCTCTATCC
AGATAGCAAAACAGGCTCAGGCGGAAGGCTTTGCAAACTTGAGGCAGTCAGCCCTTAAAAAGGTCATTGAGGGCGTGACC
AGTCTCGAAGAAGCGAACCGCGTGACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

98.774

0.578
  pilB Acinetobacter baylyi ADP1

56.991

98.949

0.564
  pilB Legionella pneumophila strain ERS1305867

54.609

100

0.55
  pilB Vibrio parahaemolyticus RIMD 2210633

52.151

97.723

0.51
  pilB Vibrio campbellii strain DS40M4

50.265

98.949

0.497
  pilB Vibrio cholerae strain A1552

55.186

89.492

0.494
  pilF Neisseria gonorrhoeae MS11

47.266

99.299

0.469
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.678

92.995

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

40.115

91.243

0.366

Multiple sequence alignment