Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CS053_RS04310 Genome accession   NZ_CP042807
Coordinates   1035027..1036748 (-) Length   573 a.a.
NCBI ID   WP_147626499.1    Uniprot ID   A0A5B9DVF6
Organism   Rhodanobacter glycinis strain T01E-68     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1030027..1041748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS053_RS04295 (CS053_04295) coaE 1031705..1032322 (-) 618 WP_147626496.1 dephospho-CoA kinase -
  CS053_RS04300 (CS053_04300) pilD 1032319..1033179 (-) 861 WP_147626497.1 A24 family peptidase Machinery gene
  CS053_RS04305 (CS053_04305) pilC 1033427..1034695 (-) 1269 WP_147626498.1 type II secretion system F family protein Machinery gene
  CS053_RS04310 (CS053_04310) pilB 1035027..1036748 (-) 1722 WP_147626499.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CS053_RS04315 (CS053_04315) - 1037373..1038227 (+) 855 WP_147626500.1 glycosyltransferase family 2 protein -
  CS053_RS04320 (CS053_04320) - 1038291..1039580 (+) 1290 WP_147626501.1 flippase -
  CS053_RS04325 (CS053_04325) - 1039753..1040790 (+) 1038 WP_172624004.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62890.86 Da        Isoelectric Point: 6.4327

>NTDB_id=378689 CS053_RS04310 WP_147626499.1 1035027..1036748(-) (pilB) [Rhodanobacter glycinis strain T01E-68]
MSSQVQPTLVGLSGMARRLVTEGMMPENEVRQAVADSSQKKVTLTNWLLDHGLVDSMVLTRLASVEFGMPMLDITAIPPA
NMPLNLISEALLSKHHALPLLKRGKRLYVGIADPMQLHALDEIKFHSNCMVEPILVERSQLNRVIESAISTMNNVMPDMG
SGELDELALESGDEEEASTGIDATGGDDAPVVKFVNKILVDAIRRGASDIHFEPFETQYRVRLRMDGMLRAVANPPMKLA
NRISSRLKVMAGLDIAERRVPQDGRIKLNLSKNRSMDFRVSSLPTLFGEKLVLRILDSSSAKLGIDKLGYEEDQKKLYLD
AIAKPYGMVLVTGPTGSGKTVSLYTALNILNTDERNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAAQTIARLMNMGIAPYNITSSVTLIIAQRLARRLHSCKKPMQLPPQVLL
DAGFAQEDIDAGLTIYEAVGCNECNEGYKGRVGIYQVMPMLEDIQKIILQGGNALQISEVARKAGINDLRASALKKVKDG
MTSLAEIDRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=378689 CS053_RS04310 WP_147626499.1 1035027..1036748(-) (pilB) [Rhodanobacter glycinis strain T01E-68]
ATGTCGTCGCAAGTACAACCAACGCTGGTAGGCCTGTCAGGCATGGCCAGACGACTGGTGACCGAAGGCATGATGCCGGA
AAACGAGGTGCGCCAGGCCGTAGCCGATTCCAGCCAGAAAAAGGTCACCCTGACCAACTGGCTGCTGGACCACGGCTTGG
TCGACAGCATGGTGTTGACCCGGTTGGCGTCAGTCGAGTTCGGCATGCCGATGCTGGACATCACGGCCATTCCGCCGGCC
AACATGCCGCTGAACCTGATTAGCGAAGCCCTGCTCAGCAAGCATCACGCGCTGCCGCTGCTCAAGCGTGGCAAGCGTCT
GTACGTGGGCATTGCCGACCCGATGCAGTTGCACGCCTTGGACGAGATCAAGTTCCACTCCAATTGCATGGTGGAGCCGA
TCCTGGTCGAGCGCAGCCAGCTCAATCGCGTGATCGAAAGCGCAATCAGCACGATGAACAACGTCATGCCCGACATGGGC
AGTGGCGAACTGGACGAGTTGGCGCTGGAGTCGGGTGACGAAGAAGAAGCCAGCACCGGCATCGATGCCACCGGCGGCGA
CGATGCGCCGGTAGTGAAGTTCGTCAACAAGATTCTGGTGGATGCCATCCGCCGCGGCGCCTCGGACATCCACTTCGAAC
CGTTCGAAACCCAGTATCGCGTGCGCCTGCGCATGGACGGCATGTTGCGCGCCGTGGCCAACCCGCCGATGAAGCTGGCC
AACCGCATTTCCTCACGATTGAAGGTCATGGCCGGCCTCGACATCGCCGAGCGCCGCGTACCGCAGGACGGGCGCATCAA
GCTCAACCTGTCCAAGAACCGCTCGATGGACTTCCGCGTCAGCTCGCTGCCTACGTTGTTCGGCGAGAAGCTGGTGCTGC
GTATCCTGGACAGTTCTTCGGCCAAGCTCGGCATCGACAAGCTGGGCTATGAGGAAGACCAGAAGAAGCTTTATCTCGAT
GCCATCGCCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACACCGCGTT
GAATATCCTCAACACCGACGAACGCAACATCTCCACCGTGGAAGACCCGGTGGAAATCCGTGTCGAAGGCATCAACCAGG
TACAGCAGAACGTGAAGCGCGGCATGACCTTTGCCGCCGCCCTGCGTTCGTTCCTGCGCCAGGACCCGGACGTGATCATG
GTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCGATCAAGGCAGCCCAGACCGGCCACATGGTGCTGTCCACGTT
GCACACCAACGATGCCGCGCAGACCATCGCCCGCCTGATGAACATGGGTATCGCGCCGTACAACATCACCTCGTCGGTCA
CCCTGATCATTGCCCAGCGCTTGGCCCGGCGCCTGCATAGCTGCAAGAAGCCGATGCAACTGCCACCGCAGGTGCTGCTG
GATGCAGGCTTCGCCCAGGAAGACATCGATGCCGGCCTCACCATCTACGAGGCGGTAGGCTGCAATGAGTGCAACGAGGG
TTACAAGGGCCGCGTGGGCATCTACCAGGTGATGCCGATGCTGGAGGACATCCAGAAGATCATCCTGCAAGGCGGCAACG
CCCTGCAGATTTCCGAAGTGGCGCGCAAGGCCGGCATCAACGACTTGCGCGCATCAGCTCTCAAGAAAGTGAAAGACGGC
ATGACCAGCCTGGCCGAGATCGACCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5B9DVF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.009

99.302

0.546
  pilB Acinetobacter baylyi ADP1

55.142

98.429

0.543
  pilB Legionella pneumophila strain ERS1305867

50.615

99.302

0.503
  pilB Vibrio cholerae strain A1552

50.184

94.939

0.476
  pilF Neisseria gonorrhoeae MS11

47.518

98.429

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

47.257

98.604

0.466
  pilB Vibrio campbellii strain DS40M4

49.067

93.543

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.779

97.208

0.377
  pilF Thermus thermophilus HB27

37.456

100

0.375

Multiple sequence alignment