Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FPL04_RS16265 Genome accession   NZ_CP041965
Coordinates   3721119..3722828 (-) Length   569 a.a.
NCBI ID   WP_186811040.1    Uniprot ID   -
Organism   Xanthomonas arboricola strain SL2098     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3716119..3727828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPL04_RS16255 (FPL04_16255) - 3717454..3719067 (+) 1614 WP_039525813.1 HAMP domain-containing sensor histidine kinase -
  FPL04_RS16260 (FPL04_16260) pilR 3719260..3720654 (+) 1395 WP_166770466.1 sigma-54 dependent transcriptional regulator Regulator
  FPL04_RS22115 - 3720951..3721094 (-) 144 WP_166770464.1 hypothetical protein -
  FPL04_RS16265 (FPL04_16265) pilB 3721119..3722828 (-) 1710 WP_186811040.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FPL04_RS16270 (FPL04_16270) - 3722912..3723367 (-) 456 WP_154832506.1 pilin -
  FPL04_RS16275 (FPL04_16275) - 3723476..3723913 (-) 438 WP_104621768.1 pilin -
  FPL04_RS16280 (FPL04_16280) pilC 3724268..3725524 (+) 1257 WP_104621769.1 type II secretion system F family protein Machinery gene
  FPL04_RS16285 (FPL04_16285) - 3725531..3726394 (+) 864 WP_016849610.1 A24 family peptidase -
  FPL04_RS16290 (FPL04_16290) coaE 3726408..3727031 (+) 624 WP_154832507.1 dephospho-CoA kinase -
  FPL04_RS16295 (FPL04_16295) - 3727131..3727286 (+) 156 Protein_3100 SymE family type I addiction module toxin -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61753.78 Da        Isoelectric Point: 5.6024

>NTDB_id=374950 FPL04_RS16265 WP_186811040.1 3721119..3722828(-) (pilB) [Xanthomonas arboricola strain SL2098]
MGITGIARRLVQDGAIEEAVARSAMDQAAAAKIPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDASQNAIKLVSE
ELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLGDDDEGMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPEHALLAEGF
TPAQIAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALVKAAHGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=374950 FPL04_RS16265 WP_186811040.1 3721119..3722828(-) (pilB) [Xanthomonas arboricola strain SL2098]
GTGGGAATCACCGGCATTGCGCGTCGTCTGGTGCAGGACGGTGCGATCGAGGAGGCGGTTGCCCGTTCCGCGATGGATCA
GGCGGCTGCGGCAAAGATCCCGTTGCCGCAGTGGTTCTCGGAGAAAAAGTTGGTATCGGCCTCGCAACTGGCTGCTGCCA
ACGCGGTCGAGTTCGGCATGCCTTTGATGGACGTGTCGGTGTTCGACGCCAGCCAGAACGCCATCAAGCTAGTCAGTGAG
GAGTTGCTCCAGAAGTATCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTTCGTGGGGGTGAGCAACCCGACCCA
GACCCGGGCGCTGGACGACATCAAGTTCCATACCAACCTGGTGGTCGAGCCGATCCTGGTCGACGAGGACCAGATCCGTC
GCACCTTGGAGCAATGGCAAGCCAGCAATGCGGCGCTTGGCTCGTCGCTCGGTGACGACGATGAGGGGATGGGCGACCTG
GACGTGTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCCACTTCGAGCCGTACGAAGACGACT
ATCGGGTGCGCTTGCGCATCGATGGGCTGCTGAAGAATGTGGCCAAGGCACCGGTAAAGCTGAACCAGCGCATCGCGGCG
CGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACGTTGTTCGGCGAGAAGGTGGTGCTGCGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTATGAGGCGGACCAGCAGAAGCTGTTCTTGGAGGCGATCCACAAGCCG
TACGGGATGGTGTTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACACGGCACTGGGCATCCTCAACGA
CGAGACGCGCAACATCTCCACCGCCGAGGACCCGGTGGAAATCCGCCTGCCTGGCGTCAATCAGGTGCAGCAGAACAACA
AGCGCGGCATGACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACGGCCGAGATTGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCTGTCCACGCTGCACACCAACGATGC
GCCGCAGACCATCGCGCGTTTGATGAACATGGGCATCGCGCCTTACAACATCACCTCGTCGGTGACCCTGGTGATCGCGC
AGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAGCATGCGTTGCTGGCCGAAGGCTTC
ACGCCGGCGCAGATCGCTGCCGGTATCGAGTTGTATGAGGCGGTCGGCTGCGACGAATGCACCGAAGGCTACAAGGGCCG
TACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGCTGGAAGGCGGTAACGCGATGCAGATCG
CCGAGGCGGCACAGAGTATCGGCATCCGCGACCTGCGCCAGTCGGCGTTGGTCAAGGCCGCGCACGGGGTAACCAGCCTG
GCCGAAATCAATCGGGTGACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

99.824

0.562
  pilB Acinetobacter baylyi ADP1

55.458

99.824

0.554
  pilB Legionella pneumophila strain ERS1305867

52.197

100

0.522
  pilB Vibrio cholerae strain A1552

48.866

100

0.492
  pilF Neisseria gonorrhoeae MS11

48.592

99.824

0.485
  pilB Vibrio campbellii strain DS40M4

45.87

100

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

98.418

0.459
  pilF Thermus thermophilus HB27

40.192

91.388

0.367
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.429

92.267

0.364

Multiple sequence alignment