Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FPK91_RS15850 Genome accession   NZ_CP041783
Coordinates   3669520..3671232 (-) Length   570 a.a.
NCBI ID   WP_144212250.1    Uniprot ID   -
Organism   Shewanella donghaensis strain LT17     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3664520..3676232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPK91_RS15820 mutT 3665187..3665579 (-) 393 WP_144212238.1 8-oxo-dGTP diphosphatase MutT -
  FPK91_RS15825 yacG 3665606..3665815 (-) 210 WP_144212240.1 DNA gyrase inhibitor YacG -
  FPK91_RS15830 zapD 3665856..3666593 (-) 738 WP_144212242.1 cell division protein ZapD -
  FPK91_RS15835 coaE 3666599..3667204 (-) 606 WP_144212244.1 dephospho-CoA kinase -
  FPK91_RS15840 pilD 3667249..3668163 (-) 915 WP_144212246.1 A24 family peptidase Machinery gene
  FPK91_RS15845 pilC 3668202..3669464 (-) 1263 WP_144212248.1 type II secretion system F family protein Machinery gene
  FPK91_RS15850 pilB 3669520..3671232 (-) 1713 WP_144212250.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FPK91_RS15855 - 3671353..3671772 (-) 420 WP_144212252.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FPK91_RS15860 nadC 3672665..3673543 (-) 879 WP_144212254.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FPK91_RS15865 ampD 3673837..3674406 (+) 570 WP_144212256.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FPK91_RS15870 ampE 3674471..3675322 (+) 852 WP_144212258.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63226.46 Da        Isoelectric Point: 5.0378

>NTDB_id=374252 FPK91_RS15850 WP_144212250.1 3669520..3671232(-) (pilB) [Shewanella donghaensis strain LT17]
MSSSGLNLGLSTLFIRKNLLSEQQVAAAISQSRKTKQSLVSSIIKDKLLSAREIAELCYEEYGAPLLDISEFDLANIPED
ILNKKLMLNHRCLPLFKRSNRLFIATSDPTNIAALEDFQFSLGLHAEAIIVEDDKLQIAIEKLLEDDISGLGLDGIDEEA
LAGIELSDNDKRNEEPEAESSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSSRISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSSLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRELYEQMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFPSALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQPEDVPQAELTRLGF
TQEMIDSDFTVYKPIGCEHCSGGYKGRVGIYEVMKMTDEIARTIMEGGNSLQIAQQAKDNGMRDLRLSGLLKVTKGITSI
VEVNRVTSFN

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=374252 FPK91_RS15850 WP_144212250.1 3669520..3671232(-) (pilB) [Shewanella donghaensis strain LT17]
ATGTCTTCATCGGGATTAAATTTAGGACTATCGACGTTATTCATCCGTAAAAATTTACTATCTGAGCAACAAGTAGCAGC
CGCAATTTCCCAATCCCGTAAAACCAAACAATCGCTTGTTAGCTCCATAATTAAAGATAAGCTTTTGTCTGCCAGAGAAA
TTGCAGAGCTTTGTTATGAAGAGTATGGTGCGCCACTACTAGATATTTCAGAGTTCGATCTAGCCAATATCCCTGAAGAC
ATACTCAATAAAAAACTGATGTTGAACCATCGCTGCCTTCCTCTATTTAAACGCAGTAATCGTTTATTTATTGCTACTTC
CGACCCCACCAATATTGCCGCTCTCGAAGACTTTCAATTTAGTCTTGGTTTGCATGCTGAAGCCATTATCGTTGAAGATG
ATAAACTTCAGATTGCTATAGAAAAACTTCTGGAAGATGACATATCCGGCCTCGGTTTAGATGGTATAGACGAAGAAGCG
CTTGCTGGCATCGAACTTTCTGATAATGATAAACGCAATGAAGAGCCTGAAGCCGAAAGCAGTGATGATGCTCCCATAGT
TATCTATATTAATAAAATTCTTACCGATGCCATCCGTAAAGGTGCTTCAGATTTACATTTTGAGCCCTACGAAAAGCGTT
ACCGTATTCGCTTCAGAATTGACGGTATATTACATGAAGTGTCCGAGCCGCCGGTAAACCTGTCCAGCAGAATATCAGCC
CGCTTAAAGGTAATGTCAAAACTGGATATTGCAGAGCGTCGCGTTCCCCAGGATGGCCGCATAAAAATGAAGCTTTCACG
GACTAAATCAATCGATTTTCGTGTCAGTTCGCTGCCTACTATTTGGGGCGAAAAAATCGTAATGCGTATCCTCGATTCAT
CTTCTGCCCAGCTAGGCATTGAGAAATTAGGCTACGAAGATGATCAACGTGAACTTTATGAGCAAATGCTCGCTAAGCCT
CAAGGCATGATTTTAGTTACAGGTCCCACAGGTTCAGGTAAAACCGTATCGCTTTATACTGGACTTAATATTCTTAACAC
CGAAGAACGTAATATCTCCACCGCAGAAGATCCGGTAGAAATCAATTTAGAGGGGGTTAACCAGGTTCACATTAATTTAA
AAGCAGGCTTAACCTTTCCTTCAGCATTAAGATCATTTTTACGTCAAGATCCCGATGTGGTGATGGTAGGTGAAATTCGA
GATTTAGAAACTGCTGAAATCGCGATTAAAGCGGCACAAACGGGTCACTTGGTTCTATCAACCTTACATACCAATTCTGC
AGCAGAAACCCTTACTCGCTTAATTAATATGGGTGTTCCGGGCTATAATATTGCCAGCTCTGTAAACTTGATTATTGCTC
AGCGTCTAGCCCGAAGATTGTGCCCTGAATGTAAACAACCCGAAGATGTTCCCCAGGCAGAATTAACGCGCTTAGGTTTT
ACTCAAGAAATGATTGATTCAGACTTTACCGTCTATAAGCCTATCGGCTGTGAACATTGTTCTGGCGGCTACAAAGGTCG
AGTGGGTATTTACGAAGTAATGAAAATGACTGACGAAATAGCCCGTACTATTATGGAAGGTGGCAACTCACTGCAAATAG
CTCAACAAGCTAAAGACAACGGCATGCGAGATTTAAGACTTTCAGGCTTACTAAAAGTCACTAAAGGCATTACCAGTATT
GTCGAGGTCAACCGAGTCACCAGTTTCAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.259

98.772

0.575
  pilB Acinetobacter baylyi ADP1

57.576

98.421

0.567
  pilB Legionella pneumophila strain ERS1305867

57.295

98.596

0.565
  pilB Vibrio cholerae strain A1552

52.113

99.649

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.957

98.596

0.512
  pilB Vibrio campbellii strain DS40M4

50.265

99.123

0.498
  pilF Neisseria gonorrhoeae MS11

46.585

100

0.467
  pilF Thermus thermophilus HB27

37.522

99.123

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.882

97.719

0.37

Multiple sequence alignment