Detailed information NTG0373885

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: FO444_RS09710
• Genome accession: NZ_CP041759
• Coordinates: 1921788..1922435 (-)
• Length: 215 a.a.
• NCBI ID: WP_017864913.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain IMAU11823
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1916788..1927435

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FO444_RS09685 (FO444_09660)	atpF	1916857..1917363 (-)	507	WP_010906128.1	F0F1 ATP synthase subunit B	-
FO444_RS09690 (FO444_09665)	atpB	1917378..1918091 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
FO444_RS09695 (FO444_09670)	-	1918136..1918351 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
FO444_RS09700 (FO444_09675)	-	1918536..1919312 (-)	777	WP_017864911.1	alpha/beta hydrolase family protein	-
FO444_RS09705 (FO444_09680)	comEC	1919597..1921807 (-)	2211	WP_058210776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
FO444_RS09710 (FO444_09685)	comEA	1921788..1922435 (-)	648	WP_017864913.1	ComEA family DNA-binding protein	Machinery gene
FO444_RS09715 (FO444_09690)	-	1922495..1923856 (-)	1362	WP_017864914.1	ABC transporter permease	-
FO444_RS09720 (FO444_09695)	-	1923853..1924785 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
FO444_RS09725 (FO444_09700)	-	1924891..1925289 (-)	399	WP_023188528.1	hypothetical protein	-
FO444_RS09730 (FO444_09705)	-	1925393..1925956 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
FO444_RS09735 (FO444_09710)	-	1926129..1927307 (-)	1179	WP_012898345.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23598.73 Da
• Isoelectric Point: 5.0013

>NTDB_id=373885 FO444_RS09710 WP_017864913.1 1921788..1922435(-) (comEA) [Lactococcus lactis subsp. lactis strain IMAU11823]
MDKILEKVKEYWKMIVLVVCGLIAGGIFHVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=373885 FO444_RS09710 WP_017864913.1 1921788..1922435(-) (comEA) [Lactococcus lactis subsp. lactis strain IMAU11823]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTCACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	38.696	100	0.414
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367