Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FNU76_RS08220 Genome accession   NZ_CP041730
Coordinates   1840506..1842218 (+) Length   570 a.a.
NCBI ID   WP_144277748.1    Uniprot ID   A0A516SDW9
Organism   Chitinimonas arctica strain R3-44     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1835506..1847218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNU76_RS08190 (FNU76_08190) rplS 1835615..1835995 (-) 381 WP_144277742.1 50S ribosomal protein L19 -
  FNU76_RS08195 (FNU76_08195) trmD 1836001..1836789 (-) 789 WP_144277743.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  FNU76_RS08200 (FNU76_08200) rimM 1836791..1837339 (-) 549 WP_144277744.1 ribosome maturation factor RimM -
  FNU76_RS08205 (FNU76_08205) rpsP 1837364..1837606 (-) 243 WP_144277745.1 30S ribosomal protein S16 -
  FNU76_RS08210 (FNU76_08210) ffh 1837697..1839046 (-) 1350 WP_144277746.1 signal recognition particle protein -
  FNU76_RS08215 (FNU76_08215) - 1839137..1839955 (+) 819 WP_144277747.1 inner membrane protein YpjD -
  FNU76_RS08220 (FNU76_08220) pilF 1840506..1842218 (+) 1713 WP_144277748.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FNU76_RS08225 (FNU76_08225) pilC 1842244..1843467 (+) 1224 WP_144277749.1 type II secretion system F family protein Machinery gene
  FNU76_RS08230 (FNU76_08230) pilD 1843472..1844344 (+) 873 WP_144277750.1 A24 family peptidase Machinery gene
  FNU76_RS08235 (FNU76_08235) coaE 1844416..1845018 (+) 603 WP_144277751.1 dephospho-CoA kinase -
  FNU76_RS08240 (FNU76_08240) zapD 1845106..1845864 (+) 759 WP_144277752.1 cell division protein ZapD -
  FNU76_RS08245 (FNU76_08245) - 1845864..1846052 (+) 189 WP_144277753.1 DNA gyrase inhibitor YacG -
  FNU76_RS08250 (FNU76_08250) - 1846145..1846903 (+) 759 WP_144277754.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 61767.06 Da        Isoelectric Point: 4.9043

>NTDB_id=373594 FNU76_RS08220 WP_144277748.1 1840506..1842218(+) (pilF) [Chitinimonas arctica strain R3-44]
MAQAPSPLSGIARALVQQGRLQEADAEAIQIQAQNGNNHFVQALVASKKLSALEVAEFASNTFGYPMLDLAQMDAAYIPS
GVLDVKLMQSRRIAPLYKRGTKLFVAISDPTFLQALEDAKFQTGTVIEPVVVEDQKLGQLIEKLVEATGSNLKDMVVDQE
DLNLAGGEEAAAEPDVGSAEVDDAPVVKYIQKILLDAINGGASDIHFEPYEKFYRIRYRIDGVLREIATPPLAIKDKMSS
RIKVISKLDISEKRVPQDGRMRLVLSKTRAIDFRVSTLPTLFGEKICMRILDPSSATLGIDALGYEPDQKAALLEAVHRP
YGMVLVTGPTGSGKTVSLYTCLNILNEPGINISTAEDPAEINLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIMVGEIR
DLETADISIKAAQTGHMVFSTLHTNDAPATLSRMLNMGIAPFNIASSVILITGQRLARKLCTACKKPLDIPPEALLSAGF
SEEDLDGSWQPYGPVGCDICKGTGYKGRVGIYQVMPISEEMQRIIMAQGNSIDIADQAKREGVRDLRQSGLLKVKQGMTS
LTEVEAVTNE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=373594 FNU76_RS08220 WP_144277748.1 1840506..1842218(+) (pilF) [Chitinimonas arctica strain R3-44]
ATGGCGCAAGCCCCTTCCCCACTCTCCGGCATCGCCCGCGCGCTCGTCCAGCAAGGCCGCCTGCAAGAGGCCGATGCGGA
AGCGATTCAGATTCAGGCGCAAAACGGCAATAACCATTTCGTACAGGCCCTGGTAGCCAGTAAAAAATTATCGGCCCTGG
AAGTTGCGGAGTTCGCTTCCAACACGTTTGGCTATCCCATGCTGGACCTGGCGCAAATGGACGCCGCCTATATTCCCTCC
GGCGTACTCGATGTCAAGCTGATGCAGAGCCGACGTATCGCGCCGCTCTACAAACGCGGCACCAAGCTATTCGTCGCCAT
TTCCGACCCGACCTTCCTGCAGGCATTGGAAGATGCCAAGTTCCAGACCGGCACCGTGATCGAGCCGGTGGTAGTGGAGG
ATCAAAAACTCGGCCAGTTGATCGAGAAGCTGGTGGAAGCGACCGGCTCCAACCTTAAAGACATGGTGGTCGATCAGGAA
GACCTCAATCTGGCGGGCGGCGAAGAAGCCGCGGCGGAACCCGACGTCGGCTCGGCCGAGGTGGATGACGCACCGGTCGT
GAAATACATCCAGAAGATCCTGCTGGACGCCATCAACGGCGGCGCCTCCGACATCCATTTCGAACCCTACGAGAAGTTCT
ACCGCATCCGCTACCGGATCGACGGGGTGCTGCGCGAAATCGCCACACCGCCGCTGGCCATCAAGGACAAGATGTCCTCG
CGGATCAAGGTGATCTCCAAGCTCGACATCTCGGAAAAGCGCGTCCCGCAGGATGGCCGCATGCGGTTGGTACTGTCCAA
GACGCGGGCGATCGACTTCCGGGTGTCCACGCTGCCCACCCTGTTCGGCGAGAAGATTTGTATGCGTATCCTGGACCCCA
GCTCCGCCACGCTGGGGATCGATGCGCTGGGTTACGAGCCGGACCAGAAGGCCGCCTTGCTGGAGGCCGTGCACCGCCCC
TACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACCGTTTCGCTATATACCTGCCTCAATATCCTGAATGA
GCCGGGTATCAATATTTCCACGGCGGAAGACCCGGCCGAAATCAACCTGCCGGGGATCAACCAGGTCAACGTGAACGACA
AGGCCGGCCTGACCTTTGCCGCCGCCTTGAAGTCGTTCCTGCGCCAGGATCCGGACATCATCATGGTGGGTGAAATCCGC
GACCTGGAAACGGCCGACATCTCCATCAAGGCCGCCCAAACCGGCCACATGGTGTTCTCCACCCTGCATACCAATGACGC
CCCGGCGACCTTGTCGCGGATGTTGAATATGGGTATCGCGCCATTCAATATCGCTTCTTCGGTCATCCTGATTACCGGCC
AACGGCTGGCGCGCAAGCTATGCACCGCCTGCAAGAAGCCGCTGGACATCCCGCCCGAAGCCTTGCTGAGCGCGGGCTTT
TCCGAAGAAGACCTCGATGGCAGCTGGCAACCTTATGGCCCGGTGGGCTGCGATATCTGCAAAGGCACCGGCTACAAGGG
ACGAGTTGGCATCTATCAGGTCATGCCGATTTCGGAAGAAATGCAGCGCATCATCATGGCGCAAGGAAATTCCATCGACA
TTGCCGACCAGGCCAAGCGCGAAGGTGTTCGCGACCTGCGCCAGTCGGGCCTGTTGAAGGTCAAACAAGGCATGACCTCG
CTGACGGAAGTCGAAGCGGTAACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A516SDW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

56.437

99.474

0.561
  pilB Acinetobacter baumannii D1279779

55.929

99.123

0.554
  pilB Acinetobacter baylyi ADP1

55.36

99.825

0.553
  pilB Legionella pneumophila strain ERS1305867

52.348

100

0.528
  pilB Vibrio parahaemolyticus RIMD 2210633

47.09

99.474

0.468
  pilB Vibrio cholerae strain A1552

47.5

98.246

0.467
  pilB Vibrio campbellii strain DS40M4

46.303

99.649

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.758

95.789

0.4
  pilF Thermus thermophilus HB27

38.011

98.772

0.375

Multiple sequence alignment