Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FNC98_RS02750 Genome accession   NZ_CP041638
Coordinates   629966..631663 (-) Length   565 a.a.
NCBI ID   WP_143579826.1    Uniprot ID   A0A516H9D9
Organism   Thalassotalea sp. PS06     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 624966..636663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FNC98_RS02725 (FNC98_02725) - 625163..625990 (-) 828 WP_143579821.1 glycerophosphodiester phosphodiesterase family protein -
  FNC98_RS02730 (FNC98_02730) - 626012..626758 (-) 747 WP_143579822.1 cell division protein ZapD -
  FNC98_RS02735 (FNC98_02735) coaE 627011..627619 (-) 609 WP_143579823.1 dephospho-CoA kinase -
  FNC98_RS02740 (FNC98_02740) pilD 627672..628571 (-) 900 WP_313904112.1 A24 family peptidase Machinery gene
  FNC98_RS02745 (FNC98_02745) pilC 628643..629890 (-) 1248 WP_143579825.1 type II secretion system F family protein Machinery gene
  FNC98_RS02750 (FNC98_02750) pilB 629966..631663 (-) 1698 WP_143579826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FNC98_RS02755 (FNC98_02755) - 631759..632181 (-) 423 WP_313904113.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FNC98_RS02760 (FNC98_02760) nadC 632701..633567 (-) 867 WP_143579828.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FNC98_RS02765 (FNC98_02765) - 633606..634133 (-) 528 WP_143579829.1 TIGR02281 family clan AA aspartic protease -
  FNC98_RS02770 (FNC98_02770) ampD 634411..634998 (+) 588 WP_143579830.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FNC98_RS02775 (FNC98_02775) ampE 635295..636140 (+) 846 WP_143579831.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 565 a.a.        Molecular weight: 62673.50 Da        Isoelectric Point: 4.9795

>NTDB_id=372675 FNC98_RS02750 WP_143579826.1 629966..631663(-) (pilB) [Thalassotalea sp. PS06]
MKGLHRQSNLLSKFVRTELIDQQQAADTFKVCQQSKQPILDYIIDELKIDSHTIAKTLSKSFGYPLVEIKDFDLDSLPEG
LRNQKLIIKHRAMPLYLRAGVLHIAVSDPTNHDALEEFQFNTGHTTEFVLVDRSSLEKQIEAYLDNEADALEITELDNEE
LSGIDVAETEDKDDDSGGENDAPIVVYINKILLDSIKKGASDLHFEPFEKKFRIRFRIDGVLQEVATPPVGLASRLTARL
KVMSKLDIAERRVPQDGRIKLNITKNKAIDFRVSTLPTMWGEKVVMRILDSSSAKLGIDILGYEEQQKAMYLEALSKPQG
MILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVQINNKAGLTFAEALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSYNVASSVSLIIAQRLARRLCNHCKEEDHVNDEALLEIGFKP
EELNDLIIYKPVGCEECTAGYKGRVGIYEVMKISEKTAEIIMQGGNSLQIANQAQQEGYNNLRQSALQKAKAGVTSLAEV
SRVTA

Nucleotide


Download         Length: 1698 bp        

>NTDB_id=372675 FNC98_RS02750 WP_143579826.1 629966..631663(-) (pilB) [Thalassotalea sp. PS06]
ATGAAAGGACTTCATCGTCAATCTAACTTATTATCAAAGTTTGTTCGTACAGAATTAATAGATCAACAACAGGCAGCTGA
CACCTTTAAAGTATGTCAGCAATCCAAGCAGCCGATTCTCGATTATATTATCGATGAGTTAAAGATTGACTCACACACGA
TTGCCAAAACATTGTCAAAGAGTTTCGGTTACCCTCTGGTCGAAATCAAAGATTTTGACTTAGATAGCCTGCCTGAAGGT
CTGCGCAATCAAAAGCTAATTATCAAACACCGCGCAATGCCACTTTATCTTAGAGCAGGCGTCCTTCACATTGCCGTATC
CGACCCAACCAATCATGATGCACTGGAAGAGTTCCAGTTCAATACCGGCCATACCACTGAATTCGTACTTGTCGATCGTT
CCAGCCTTGAAAAGCAAATTGAAGCCTACCTGGATAACGAAGCGGATGCGCTAGAAATTACCGAACTTGATAACGAAGAG
CTCAGCGGTATAGACGTTGCTGAAACAGAAGACAAAGACGATGACTCCGGTGGTGAAAATGATGCACCTATCGTTGTTTA
TATTAATAAAATCTTACTTGATTCAATAAAAAAAGGCGCATCTGATTTACATTTTGAGCCATTTGAGAAGAAGTTTCGCA
TCCGCTTTCGTATCGATGGGGTGTTACAAGAGGTCGCCACTCCACCGGTAGGCCTCGCCAGCCGGTTAACCGCCCGATTA
AAAGTTATGTCGAAACTCGATATTGCCGAACGTCGGGTTCCTCAGGATGGTCGAATAAAGCTAAATATTACTAAGAATAA
AGCTATCGATTTTCGTGTTTCCACCCTGCCCACCATGTGGGGCGAAAAGGTAGTAATGCGTATCCTGGATTCTTCCAGCG
CAAAACTTGGTATCGATATTCTCGGTTACGAAGAACAACAAAAAGCCATGTATCTGGAGGCACTCAGTAAGCCTCAGGGT
ATGATCCTGGTAACCGGTCCAACCGGTTCCGGTAAAACCGTATCCCTGTACACAGGTTTGAACATTCTCAATACTGAAGA
ACGCAATATCTCCACCGCCGAAGACCCGGTAGAAATTAACCTTGAAGGGGTGAACCAGGTTCAGATCAATAACAAGGCAG
GGTTGACTTTCGCAGAAGCGTTAAAATCTTTCCTGCGTCAGGATCCGGATATCGTCATGGTGGGTGAGATACGGGATTTG
GAAACCGCAGAAATCGCCATTAAAGCCGCGCAAACCGGTCACTTAGTATTATCTACCCTGCACACTAACTCTGCGGCAGA
AACCTTAACCCGCTTGCTAAATATGGGGGTGCCTTCCTATAACGTTGCCAGTTCAGTGAGTCTGATTATTGCCCAGCGAC
TCGCCAGACGTTTGTGTAACCATTGTAAAGAAGAAGACCATGTCAATGATGAGGCGTTACTGGAAATCGGCTTTAAACCG
GAAGAGTTAAACGATTTGATCATATACAAACCAGTTGGTTGTGAGGAATGCACTGCCGGTTATAAAGGCCGAGTCGGCAT
CTACGAAGTGATGAAAATTTCTGAAAAAACTGCGGAAATTATTATGCAGGGCGGTAACTCTCTGCAGATTGCCAATCAGG
CCCAACAGGAGGGTTATAACAACCTTCGCCAATCGGCATTGCAAAAAGCAAAAGCAGGTGTCACTAGTCTTGCAGAAGTC
AGCAGGGTTACGGCATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A516H9D9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.194

99.646

0.57
  pilB Acinetobacter baumannii D1279779

55.401

100

0.563
  pilB Legionella pneumophila strain ERS1305867

55.576

98.407

0.547
  pilB Vibrio cholerae strain A1552

52.043

99.646

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

54.913

91.858

0.504
  pilB Vibrio campbellii strain DS40M4

53.861

91.681

0.494
  pilF Neisseria gonorrhoeae MS11

47.662

98.407

0.469
  pilF Thermus thermophilus HB27

35.417

100

0.361

Multiple sequence alignment