Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FM037_RS01710 Genome accession   NZ_CP041614
Coordinates   385812..387521 (-) Length   569 a.a.
NCBI ID   WP_144044572.1    Uniprot ID   -
Organism   Shewanella psychropiezotolerans strain YLB-06     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 380812..392521
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FM037_RS01680 (FM037_01680) mutT 381347..381757 (-) 411 WP_144044566.1 8-oxo-dGTP diphosphatase MutT -
  FM037_RS01685 (FM037_01685) yacG 381763..381975 (-) 213 WP_144044567.1 DNA gyrase inhibitor YacG -
  FM037_RS01690 (FM037_01690) zapD 382089..382823 (-) 735 WP_144044568.1 cell division protein ZapD -
  FM037_RS01695 (FM037_01695) coaE 382832..383437 (-) 606 WP_144044569.1 dephospho-CoA kinase -
  FM037_RS01700 (FM037_01700) pilD 383482..384393 (-) 912 WP_144044570.1 prepilin peptidase Machinery gene
  FM037_RS01705 (FM037_01705) pilC 384461..385732 (-) 1272 WP_144044571.1 type II secretion system F family protein Machinery gene
  FM037_RS01710 (FM037_01710) pilB 385812..387521 (-) 1710 WP_144044572.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FM037_RS01715 (FM037_01715) - 387633..388067 (-) 435 WP_144044573.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FM037_RS01720 (FM037_01720) nadC 388986..389903 (-) 918 WP_144044574.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FM037_RS01725 (FM037_01725) ampD 390232..390807 (+) 576 WP_407695647.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FM037_RS01730 (FM037_01730) ampE 390904..391755 (+) 852 WP_144044576.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63060.24 Da        Isoelectric Point: 4.9594

>NTDB_id=372443 FM037_RS01710 WP_144044572.1 385812..387521(-) (pilB) [Shewanella psychropiezotolerans strain YLB-06]
MPTTGLHLGLSTLFIRKNLLTEEQIATAIAKSRQNKQSLVSTIVSEKLISAREIAELCYEEYGTPLLDLDEFDIASIPED
FLNKKLIEKHKCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEDDKLTKAIEKVLEEDISALDISGIDESA
LADIEVDDPSRREEEQKGESGDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSARISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRELYEEMLEKP
QGMILVTGPTGSGKTVSLYTGLNILNTEARNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVAGYNIASSVNLIIAQRLARRLCPECKQAEDVPEHELQKLGF
KQANIDEGITTYKPVGCDLCSGGYKGRVGIYEVMKISDEIARIIMEGGNSLEIATMAKEQGMRDLRDSGLLKIIHGVTSI
AEINRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=372443 FM037_RS01710 WP_144044572.1 385812..387521(-) (pilB) [Shewanella psychropiezotolerans strain YLB-06]
ATGCCAACAACAGGCTTGCATTTAGGTCTATCCACGCTTTTCATTCGTAAAAATTTGCTCACCGAAGAGCAGATAGCCAC
AGCTATAGCCAAATCCCGCCAGAATAAGCAATCTCTCGTCTCTACTATAGTCTCCGAAAAGCTTATTTCTGCTCGTGAAA
TAGCCGAACTCTGTTACGAGGAATATGGCACGCCACTGCTGGACCTCGATGAATTTGATATTGCCAGCATTCCCGAAGAT
TTCCTCAACAAGAAGCTGATAGAGAAACATAAGTGCCTGCCGCTGTTTAAGCGCGGTAACCGCCTCTACATAGGCACTTC
AGATCCTACCAATATTGCAGCACTGGAAGACTTTCAATTTAGCGCGGGTCTGCATGCCGAAGCCATACTGGTCGAAGATG
ACAAGCTCACCAAGGCCATAGAGAAGGTACTCGAGGAAGATATATCTGCACTTGATATCAGTGGCATAGATGAATCGGCC
CTTGCCGATATCGAGGTCGATGATCCAAGTAGGCGCGAAGAAGAACAAAAAGGTGAGAGCGGCGACGATGCACCTATCGT
CATCTATATCAATAAAATTTTGACCGATGCCATACGTAAAGGCGCATCGGATCTGCATTTTGAACCCTATGAGAAACGTT
ACCGTATACGTTTCCGTATCGATGGTATCTTGCATGAGGTTTCCGAACCTCCGGTAAATCTTTCTGCCCGAATTTCGGCT
CGACTCAAGGTGATGTCCAAGCTAGATATCGCAGAACGAAGAGTCCCGCAAGATGGCCGTATCAAGATGAAGCTGTCTCG
CACTAAGTCCATAGATTTTCGTGTCAGCACCCTGCCTACTATCTGGGGTGAAAAGATAGTGATGCGTATCCTCGATTCCT
CCTCGGCGCAACTGGGCATCGAGAAATTGGGCTATGAAGACGATCAACGCGAGTTGTACGAGGAGATGTTAGAAAAGCCC
CAGGGCATGATTCTGGTCACTGGCCCTACCGGCTCGGGCAAAACCGTTTCTCTCTATACTGGCCTTAACATACTCAATAC
CGAAGCTCGAAATATCTCTACCGCAGAGGATCCAGTGGAAATTAACCTCGAAGGGGTTAACCAGGTCCATATCAATCTCA
AGGCGGGCCTGACATTTGCCTCTGCGTTACGCTCATTCCTGCGCCAAGATCCCGATGTAGTTATGGTCGGTGAGATTCGA
GACTTAGAGACTGCCGAGATTGCCATCAAGGCGGCACAAACCGGCCACTTAGTACTGTCGACGCTACACACCAACTCTGC
CGCCGAAACCTTGACCCGCCTAATCAACATGGGCGTAGCCGGCTATAACATAGCCAGCTCGGTAAATTTGATCATAGCTC
AGCGTTTGGCACGTCGCCTCTGTCCGGAATGTAAGCAAGCAGAAGATGTTCCAGAACATGAGCTCCAGAAGCTTGGCTTT
AAGCAAGCTAACATAGATGAAGGCATCACCACTTATAAACCTGTGGGCTGCGATCTCTGCTCCGGCGGTTACAAGGGCCG
GGTCGGCATCTATGAGGTGATGAAGATATCAGATGAGATAGCGCGCATTATCATGGAAGGTGGTAACTCCTTAGAGATAG
CCACTATGGCAAAAGAGCAGGGAATGCGAGACCTGAGAGATTCAGGGCTGCTCAAGATCATCCATGGCGTCACCAGCATC
GCCGAAATCAATCGAGTCACCAGTTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.645

98.594

0.578
  pilB Acinetobacter baumannii D1279779

57.726

98.946

0.571
  pilB Legionella pneumophila strain ERS1305867

55.3

99.473

0.55
  pilB Vibrio cholerae strain A1552

53.707

97.188

0.522
  pilB Vibrio parahaemolyticus RIMD 2210633

51.748

100

0.52
  pilB Vibrio campbellii strain DS40M4

51.681

99.297

0.513
  pilF Neisseria gonorrhoeae MS11

47.535

99.824

0.475
  pilF Thermus thermophilus HB27

39.184

99.121

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.343

97.891

0.366

Multiple sequence alignment