Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G5V60_RS19625 Genome accession   NZ_CP049101
Coordinates   4007408..4008793 (+) Length   461 a.a.
NCBI ID   WP_097478763.1    Uniprot ID   -
Organism   Escherichia coli strain EC28     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4002408..4013793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5V60_RS19600 (G5V60_19585) aroP 4002958..4004328 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  G5V60_RS19605 (G5V60_19590) ampE 4004371..4005225 (-) 855 WP_000171981.1 beta-lactamase regulator AmpE -
  G5V60_RS19610 (G5V60_19595) ampD 4005222..4005773 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G5V60_RS19615 (G5V60_19600) nadC 4005861..4006754 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G5V60_RS19620 (G5V60_19605) pilA 4006958..4007398 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  G5V60_RS19625 (G5V60_19610) pilB 4007408..4008793 (+) 1386 WP_097478763.1 type II secretion system protein GspE Machinery gene
  G5V60_RS19630 (G5V60_19615) hofC 4008783..4009985 (+) 1203 WP_164699404.1 protein transport protein HofC -
  G5V60_RS19635 (G5V60_19620) guaC 4010020..4011063 (-) 1044 WP_001217320.1 GMP reductase -
  G5V60_RS25745 - 4011219..4011263 (-) 45 WP_120795372.1 protein YacM -
  G5V60_RS19640 (G5V60_19625) coaE 4011288..4011908 (+) 621 WP_001269520.1 dephospho-CoA kinase -
  G5V60_RS19645 (G5V60_19630) zapD 4011908..4012651 (+) 744 WP_001523972.1 cell division protein ZapD -
  G5V60_RS19650 (G5V60_19635) yacG 4012661..4012858 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G5V60_RS19655 (G5V60_19640) mutT 4012946..4013344 (-) 399 WP_000736015.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50668.26 Da        Isoelectric Point: 6.5786

>NTDB_id=372430 G5V60_RS19625 WP_097478763.1 4007408..4008793(+) (pilB) [Escherichia coli strain EC28]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAHSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=372430 G5V60_RS19625 WP_097478763.1 4007408..4008793(+) (pilB) [Escherichia coli strain EC28]
ATGAATATTCCACAGCTCACTGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACATTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAGTCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAATCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAACACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACGCTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGATTAAACCAGACGCAAATCCATTC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGATCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATAAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CGTCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

49.74

83.297

0.414
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Acinetobacter baylyi ADP1

39.744

100

0.403
  pilB Vibrio campbellii strain DS40M4

48.187

83.731

0.403
  pilB Vibrio cholerae strain A1552

46.41

84.599

0.393
  pilB Acinetobacter baumannii D1279779

40.089

97.397

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

45.897

84.599

0.388
  pilB Haemophilus influenzae 86-028NP

44.961

83.948

0.377
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Haemophilus influenzae Rd KW20

44.186

83.948

0.371