Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G5V60_RS19620 Genome accession   NZ_CP049101
Coordinates   4006958..4007398 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain EC28     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4001958..4012398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G5V60_RS19600 (G5V60_19585) aroP 4002958..4004328 (+) 1371 WP_000399017.1 aromatic amino acid transporter AroP -
  G5V60_RS19605 (G5V60_19590) ampE 4004371..4005225 (-) 855 WP_000171981.1 beta-lactamase regulator AmpE -
  G5V60_RS19610 (G5V60_19595) ampD 4005222..4005773 (-) 552 WP_000923703.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G5V60_RS19615 (G5V60_19600) nadC 4005861..4006754 (+) 894 WP_001135168.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G5V60_RS19620 (G5V60_19605) pilA 4006958..4007398 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  G5V60_RS19625 (G5V60_19610) pilB 4007408..4008793 (+) 1386 WP_097478763.1 type II secretion system protein GspE Machinery gene
  G5V60_RS19630 (G5V60_19615) hofC 4008783..4009985 (+) 1203 WP_164699404.1 protein transport protein HofC -
  G5V60_RS19635 (G5V60_19620) guaC 4010020..4011063 (-) 1044 WP_001217320.1 GMP reductase -
  G5V60_RS25745 - 4011219..4011263 (-) 45 WP_120795372.1 protein YacM -
  G5V60_RS19640 (G5V60_19625) coaE 4011288..4011908 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=372429 G5V60_RS19620 WP_000360914.1 4006958..4007398(+) (pilA) [Escherichia coli strain EC28]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=372429 G5V60_RS19620 WP_000360914.1 4006958..4007398(+) (pilA) [Escherichia coli strain EC28]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGTAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363