Detailed information NTG0371458

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: IHMA87_RS24910
• Genome accession: NZ_CP041354
• Coordinates: 5192618..5194321 (+)
• Length: 567 a.a.
• NCBI ID: WP_033998207.1
• Uniprot ID: A6VBR2
• Organism: Pseudomonas aeruginosa strain AZPAE15042
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5187618..5199321

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IHMA87_RS24875 (IHMA87_04861)	-	5187860..5188135 (+)	276	WP_012077290.1	head-tail connector protein	-
IHMA87_RS24880 (IHMA87_04862)	-	5188132..5188446 (+)	315	WP_034081329.1	phage head closure protein	-
IHMA87_RS24890	-	5188872..5189054 (+)	183	WP_123809553.1	hypothetical protein	-
IHMA87_RS24895 (IHMA87_04864)	tfpZ	5189174..5189926 (-)	753	WP_034081328.1	TfpX/TfpZ family type IV pilin accessory protein	-
IHMA87_RS24900 (IHMA87_04865)	-	5189923..5191863 (-)	1941	WP_034081327.1	hypothetical protein	-
IHMA87_RS24905 (IHMA87_04866)	-	5191928..5192395 (-)	468	WP_034081326.1	pilin	-
IHMA87_RS24910 (IHMA87_04867)	pilB	5192618..5194321 (+)	1704	WP_033998207.1	type IV-A pilus assembly ATPase PilB	Machinery gene
IHMA87_RS24915 (IHMA87_04868)	pilC	5194325..5195542 (+)	1218	WP_034081324.1	type II secretion system F family protein	Machinery gene
IHMA87_RS24920 (IHMA87_04869)	pilD	5195545..5196417 (+)	873	WP_012077297.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
IHMA87_RS24925 (IHMA87_04870)	coaE	5196414..5197025 (+)	612	WP_003152767.1	dephospho-CoA kinase	-
IHMA87_RS24930 (IHMA87_04871)	yacG	5197022..5197222 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-
IHMA87_RS24935 (IHMA87_04872)	-	5197260..5197469 (-)	210	WP_033998211.1	hypothetical protein	-
IHMA87_RS24940 (IHMA87_04873)	-	5197575..5198264 (-)	690	WP_012077298.1	energy-coupling factor ABC transporter permease	-
IHMA87_RS24945 (IHMA87_04874)	-	5198261..5198731 (-)	471	WP_079381791.1	FAD/FMN-containing dehydrogenase	-
IHMA87_RS24950 (IHMA87_04875)	-	5198728..5199153 (-)	426	WP_003152776.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 567 a.a.
• Molecular weight: 62612.45 Da
• Isoelectric Point: 5.5587

>NTDB_id=371458 IHMA87_RS24910 WP_033998207.1 5192618..5194321(+) (pilB) [Pseudomonas aeruginosa strain AZPAE15042]
MNDRHPLTGLARQLVLSNLLDENAAQQAHLQAQRSKLPLVTYLVQNKLVKSRDLLELTAEQFGIAYVDLGALDKESFPKD
LISEKLSRQHRVVPLWRRGNKLFIGLSDPANHQAVSDIQFSTGLTTEAILVEDDKLTQTIDKLYENATDSLAGLDDVDLD
GLDVSTGDSSPQEDGSGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPRETLLHEGFP
EELIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRSSGLLKAMQGITSLEE
VNRVTKD

Nucleotide

• Length: 1704 bp

>NTDB_id=371458 IHMA87_RS24910 WP_033998207.1 5192618..5194321(+) (pilB) [Pseudomonas aeruginosa strain AZPAE15042]
ATGAATGATCGCCACCCCTTGACCGGACTGGCCAGGCAACTGGTTCTTTCCAATCTTCTGGATGAAAATGCCGCCCAGCA
GGCCCATTTGCAAGCCCAGCGCAGCAAGCTGCCCCTGGTCACCTATCTGGTACAGAACAAGCTGGTCAAGAGTCGCGATT
TGCTGGAACTGACAGCTGAACAGTTCGGCATCGCTTACGTAGATCTTGGTGCTCTGGACAAGGAGAGTTTCCCCAAGGAT
CTGATCAGCGAAAAACTCTCTCGACAGCACCGAGTCGTTCCACTGTGGCGACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCCGCCAACCACCAGGCGGTGTCTGACATTCAGTTCAGCACCGGGCTGACCACGGAAGCGATCCTGGTCGAAGACG
ACAAGCTGACGCAGACCATCGACAAGCTTTATGAAAATGCCACCGACAGCCTGGCTGGCCTGGACGATGTCGATCTCGAC
GGCCTGGATGTATCCACCGGCGACAGTTCGCCCCAGGAGGACGGTTCGGGCGGAGACGCCGACGACGCACCGGTAGTGCG
CTTCGTCAACAAGATGTTGCTGGACGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAACCCTACGAGAAGATCTACC
GGGTGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGCTGGCTAGCCGTATATCTGCGCGT
CTCAAGGTGATGGCCGGCCTCGACATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGATTTCCGGGTCAACACCCTGCCGACCCTCTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCA
GCGCGCAAATGGGCATCGATGCGCTGGGCTACGAAGAGGACCAGAAGGAACTCTACCTGGCCGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACGGTCTCGCTGTACACCGGCCTGAACATCCTCAATACAAC
CGATATCAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTCGGCGAGATCCGCGAC
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTCACCCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GCCTTGCGCGAAAACTCTGTTCGCATTGCAAGAAAGAACACGATGTTCCGAGGGAAACCCTGCTTCACGAGGGCTTCCCT
GAAGAACTGATCGGCACCTTCAAGCTGTATTCGCCGGTGGGCTGTGAAAATTGCAAAGGCGGTTACAAAGGTCGCGTCGG
TATTTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCTCCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

3D structure

• AlphaFold DB: A6VBR2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.511	99.471	0.582
pilB	Acinetobacter baylyi ADP1	57.549	99.295	0.571
pilB	Legionella pneumophila strain ERS1305867	55.342	100	0.557
pilB	Vibrio cholerae strain A1552	50.261	100	0.51
pilF	Neisseria gonorrhoeae MS11	49.647	99.824	0.496
pilB	Vibrio parahaemolyticus RIMD 2210633	51.201	95.414	0.489
pilB	Vibrio campbellii strain DS40M4	48.85	99.647	0.487
pilB/pilB1	Synechocystis sp. PCC 6803	38.745	95.591	0.37
pilF	Thermus thermophilus HB27	39.434	93.474	0.369
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.899	92.945	0.362