Detailed information NTG0371302

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: FLM21_RS05345
• Genome accession: NZ_CP041335
• Coordinates: 1099477..1101186 (-)
• Length: 569 a.a.
• NCBI ID: WP_148714574.1
• Uniprot ID: -
• Organism: Chitinolyticbacter meiyuanensis strain SYBC-H1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1094477..1106186

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FLM21_RS05315	-	1094882..1095631 (-)	750	WP_148714568.1	GNAT family N-acetyltransferase	-
FLM21_RS05320	-	1095737..1095916 (-)	180	WP_222846779.1	DNA gyrase inhibitor YacG	-
FLM21_RS05325	zapD	1095913..1096671 (-)	759	WP_148714570.1	cell division protein ZapD	-
FLM21_RS05330	coaE	1096732..1097355 (-)	624	WP_148714571.1	dephospho-CoA kinase	-
FLM21_RS05335	pilD	1097358..1098224 (-)	867	WP_148714572.1	A24 family peptidase	Machinery gene
FLM21_RS05340	pilC	1098230..1099462 (-)	1233	WP_148714573.1	type II secretion system F family protein	Machinery gene
FLM21_RS05345	pilB	1099477..1101186 (-)	1710	WP_148714574.1	type IV-A pilus assembly ATPase PilB	Machinery gene
FLM21_RS05350	-	1101472..1102272 (-)	801	WP_187360100.1	inner membrane protein YpjD	-
FLM21_RS05355	ffh	1102359..1103708 (+)	1350	WP_148714576.1	signal recognition particle protein	-
FLM21_RS05360	rpsP	1103809..1104057 (+)	249	WP_148714577.1	30S ribosomal protein S16	-
FLM21_RS05365	rimM	1104127..1104609 (+)	483	WP_148717453.1	ribosome maturation factor RimM	-
FLM21_RS05370	trmD	1104611..1105387 (+)	777	WP_187360101.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
FLM21_RS05375	rplS	1105444..1105827 (+)	384	WP_148714578.1	50S ribosomal protein L19	-

Sequence

Protein

• Length: 569 a.a.
• Molecular weight: 61842.11 Da
• Isoelectric Point: 5.1942

>NTDB_id=371302 FLM21_RS05345 WP_148714574.1 1099477..1101186(-) (pilB) [Chitinolyticbacter meiyuanensis strain SYBC-H1]
MSTTTAPLSGLARLLVQHGRLIEADAEALHQSATSNKLSFIEQLISSKKMSAKDVAEFGAQAFGYPLFDLDQIDSSYIPQ
GVLDPKVMQAQRVVPLYKRGTKLFVGISDITNLQALEEVRFQSGLQVEPIVVEDGKLVVLLGKLLEATGSTLKDLDVNEA
DLDISAGDEPSAPDAVSADVDDAPVVKYLQKILLDAINAGASDIHFEPYEKYYRIRYRQDGILREIAQPPLAIKEKLASR
IKVISKLDISEKRVPQDGRMKLVLSKSRAIDFRVSTLPTLHGEKICIRILDPTSATLGIDALGYDPDQKEALMEAIRRPY
GMVLVTGPTGSGKTVSLYTCLNILNEPGINISTAEDPAEINLPGINQVNVNDKAGLTFSAALKAFLRQDPDVIMVGEIRD
LETADIAIKAAQTGHMVFSTLHTNDAPTTLTRLLNMGVAPFNVASSVILITAQRLGRRLCAQCKVPVDIPPEALLDAGFS
NADLDGSWQPYRPVGCDACKGTGYKGRVGIYQVMPISDEMNRIIMKNGTAIDIADQARREGVRDLRHSGLIKVKQGLTSL
EEVMAVTNE

Nucleotide

• Length: 1710 bp

>NTDB_id=371302 FLM21_RS05345 WP_148714574.1 1099477..1101186(-) (pilB) [Chitinolyticbacter meiyuanensis strain SYBC-H1]
ATGTCCACCACCACTGCCCCGCTTTCCGGACTCGCCCGCCTCCTGGTCCAGCACGGCCGCCTGATCGAGGCCGACGCCGA
AGCCTTGCACCAGAGCGCGACGAGCAACAAGCTCAGCTTTATCGAGCAACTAATCAGCAGCAAGAAGATGTCCGCCAAGG
ACGTTGCGGAATTTGGCGCCCAGGCGTTTGGCTATCCCTTGTTCGATCTCGACCAGATTGACAGCAGCTATATCCCGCAA
GGCGTGCTCGACCCCAAGGTGATGCAGGCCCAGCGGGTAGTACCGCTATACAAGCGCGGCACCAAGCTGTTCGTCGGCAT
CTCGGACATCACCAACCTGCAGGCACTCGAAGAAGTTCGTTTCCAGTCCGGCTTGCAGGTCGAACCCATCGTCGTCGAGG
ATGGGAAACTGGTGGTGCTGCTCGGCAAATTGCTCGAAGCGACCGGATCCACACTCAAGGATCTCGACGTCAACGAGGCC
GATCTCGATATCAGTGCTGGCGATGAACCGTCCGCCCCGGATGCCGTCAGTGCCGACGTCGACGATGCACCGGTAGTGAA
ATACCTGCAGAAGATTCTGCTTGATGCAATCAATGCTGGCGCCTCGGATATCCATTTCGAGCCGTACGAGAAGTACTACC
GCATCCGCTATCGCCAAGATGGCATATTGCGCGAGATCGCCCAGCCCCCTTTGGCGATCAAGGAAAAGCTTGCATCGCGG
ATCAAGGTGATCTCCAAGCTCGACATCTCGGAAAAGCGCGTGCCGCAGGATGGCCGGATGAAACTCGTCCTCTCCAAGAG
CCGCGCCATCGATTTCCGGGTCTCGACGCTACCAACCCTGCACGGCGAGAAGATCTGTATCCGGATTCTGGACCCGACCT
CGGCCACCCTCGGCATTGATGCCCTAGGCTACGACCCGGACCAGAAAGAAGCGCTGATGGAGGCAATCCGACGTCCTTAC
GGCATGGTGCTGGTCACAGGACCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCTGCCTCAATATCCTGAACGAACC
GGGCATCAATATTTCGACCGCCGAGGATCCGGCGGAAATCAATTTGCCGGGCATCAACCAAGTCAACGTCAACGACAAGG
CCGGGCTGACTTTTTCGGCAGCCCTCAAGGCCTTTCTGCGCCAAGATCCGGACGTGATCATGGTCGGTGAAATCCGTGAC
CTCGAAACCGCCGATATTGCGATCAAGGCCGCACAGACCGGCCACATGGTGTTCTCGACGCTGCACACCAACGACGCACC
GACCACGCTGACCCGTCTGTTGAACATGGGTGTCGCGCCGTTCAACGTGGCCTCGTCAGTCATTCTGATTACCGCGCAAC
GGCTGGGCCGACGCCTGTGCGCACAATGCAAGGTGCCGGTAGATATCCCTCCTGAGGCACTGCTCGACGCTGGATTCAGC
AACGCCGATCTCGATGGCAGCTGGCAACCGTATCGACCCGTTGGCTGTGATGCCTGCAAGGGTACCGGCTATAAGGGCCG
GGTCGGCATTTACCAGGTCATGCCAATTTCCGACGAGATGAATCGCATCATCATGAAGAATGGCACGGCCATCGATATCG
CAGACCAGGCGCGGCGTGAAGGCGTACGCGATTTGCGGCATTCCGGCTTGATCAAGGTCAAGCAAGGCCTGACCTCACTC
GAAGAAGTGATGGCTGTCACCAACGAATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (191-458)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (45-146)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	56.195	100	0.566
pilB	Acinetobacter baylyi ADP1	55.497	100	0.559
pilF	Neisseria gonorrhoeae MS11	55.694	98.77	0.55
pilB	Legionella pneumophila strain ERS1305867	52.797	100	0.531
pilB	Vibrio cholerae strain A1552	48.404	99.121	0.48
pilB	Vibrio parahaemolyticus RIMD 2210633	47.788	99.297	0.475
pilB	Vibrio campbellii strain DS40M4	46.996	99.473	0.467
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.781	96.309	0.383
pilF	Thermus thermophilus HB27	38.652	99.121	0.383