Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FLK62_RS00900 Genome accession   NZ_CP041334
Coordinates   185908..187293 (+) Length   461 a.a.
NCBI ID   WP_176443368.1    Uniprot ID   A0A859IDA6
Organism   Glaesserella parasuis strain HPS412     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 180908..192293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLK62_RS00875 (FLK62_00870) gpt 181233..181712 (+) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  FLK62_RS00880 (FLK62_00875) - 182113..183084 (+) 972 WP_176443366.1 transposase -
  FLK62_RS00885 (FLK62_00880) gmk 183153..183779 (+) 627 WP_021113158.1 guanylate kinase -
  FLK62_RS00890 (FLK62_00885) radA 183832..185208 (-) 1377 WP_005711784.1 DNA repair protein RadA -
  FLK62_RS00895 (FLK62_00890) pilA 185396..185860 (+) 465 WP_176443367.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FLK62_RS00900 (FLK62_00895) pilB 185908..187293 (+) 1386 WP_176443368.1 GspE/PulE family protein Machinery gene
  FLK62_RS00905 (FLK62_00900) pilC 187286..188482 (+) 1197 WP_149350581.1 type II secretion system F family protein Machinery gene
  FLK62_RS00910 (FLK62_00905) - 188479..189147 (+) 669 WP_035491631.1 prepilin peptidase -
  FLK62_RS00915 (FLK62_00910) dapE 189231..190364 (+) 1134 WP_176443369.1 succinyl-diaminopimelate desuccinylase -
  FLK62_RS00920 (FLK62_00915) clpX 190438..191688 (-) 1251 WP_038514710.1 ATP-dependent protease ATP-binding subunit ClpX -
  FLK62_RS00925 (FLK62_00920) clpP 191695..192276 (-) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52746.52 Da        Isoelectric Point: 5.1496

>NTDB_id=371265 FLK62_RS00900 WP_176443368.1 185908..187293(+) (pilB) [Glaesserella parasuis strain HPS412]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPFYEESELAFAEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNKMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALLAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGTGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNETEVDRVLGYE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=371265 FLK62_RS00900 WP_176443368.1 185908..187293(+) (pilB) [Glaesserella parasuis strain HPS412]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCTGTGGTGATTGCTTCTGATGAG
CTTAAATACTTGCTTAATGCACTCTCGCCTGAGCAACAGCCCTTTTATGAAGAGAGCGAACTGGCTTTCGCAGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CCGACATTCATATTGAACCACGCAAGCAAAAATTGATTATTCGCCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACTCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GAGAAAAACTGGTATTACGCCTACAAAAAAACAAACCGACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCCTGTATAGTGCCTTAAACTATCTTAACAAAATGGATAAACATATTCTCACTGCCGAAGATCCCGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTATTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATTAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCACAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGATTTTTCCAGCCTACGAGAGAGTGCTAAACAGAAAATTGAACAACAACTCACCAATGAAACCGAAG
TGGATAGGGTATTAGGTTATGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-390)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-92)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A859IDA6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

97.18

100

0.972
  pilB Haemophilus influenzae 86-028NP

56.957

99.783

0.568
  pilB Haemophilus influenzae Rd KW20

57.582

98.698

0.568
  pilB Vibrio cholerae strain A1552

38.6

100

0.419
  pilB Vibrio parahaemolyticus RIMD 2210633

39.256

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.337

100

0.412
  pilB Legionella pneumophila strain ERS1305867

38.008

100

0.406
  pilB Acinetobacter baylyi ADP1

36.832

100

0.403
  pilB Acinetobacter baumannii D1279779

36.187

100

0.403
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.511

100

0.393
  pilF Neisseria gonorrhoeae MS11

36.632

100

0.377
  pilF Thermus thermophilus HB27

35.685

100

0.373

Multiple sequence alignment