Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LPA_RS05030 Genome accession   NC_014125
Coordinates   1097232..1098296 (+) Length   354 a.a.
NCBI ID   WP_013101265.1    Uniprot ID   A0A378K321
Organism   Legionella pneumophila 2300/99 Alcoy     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1092232..1103296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPA_RS05020 (lpa_01396) - 1093270..1095654 (-) 2385 WP_027226227.1 penicillin-binding protein 1A -
  LPA_RS05025 (lpa_01397) - 1095808..1096815 (-) 1008 WP_013101264.1 hypothetical protein -
  LPA_RS05030 (lpa_01398) pilM 1097232..1098296 (+) 1065 WP_013101265.1 type IV pilus assembly protein PilM Machinery gene
  LPA_RS05035 (lpa_01400) pilN 1098300..1098848 (+) 549 WP_013101266.1 PilN domain-containing protein Machinery gene
  LPA_RS05040 (lpa_01401) pilO 1098862..1099461 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  LPA_RS05045 (lpa_01402) pilP 1099458..1100042 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  LPA_RS05050 (lpa_01404) pilQ 1100046..1102142 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  LPA_RS05055 (lpa_01405) aroK 1102589..1103116 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38829.84 Da        Isoelectric Point: 5.0367

>NTDB_id=37126 LPA_RS05030 WP_013101265.1 1097232..1098296(+) (pilM) [Legionella pneumophila 2300/99 Alcoy]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPTSALEGNMIKDVDVVANCIKKLVERLRFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPAGGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINNDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=37126 LPA_RS05030 WP_013101265.1 1097232..1098296(+) (pilM) [Legionella pneumophila 2300/99 Alcoy]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTTTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGGGAAGAAATACCAACCTCTGCTCTTGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAGGTTTACATCTAAAAATGTGGCATTG
GCTGTGCCTGATTCAGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGATTGACTGATCTTGAAATGGAGGAGCTTGT
CGTTATTGAGGCGGATAAGTATATCCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGTTCATCACTGCTTGATGTTTTAATTGTGGCTTCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCTCATAGA
GCAGGGTTGGAACCTAAAATTGTTGATGTTGAATCTTATGCAGTAGAGCGCTCAGTACAACAAATTGCGCAAGAATTACC
AGCTGGGGGACAAGACAAAATTGTGGCAATAGTAGATATTGGAGCCAATTATACTCACTTGTTTGTTTTGCACGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGTGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGTTTTGTAGACCATATTTTATTGG
CAGGAGGAATGGCCAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAACCCG
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAATGATGCGCCTACACTAATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A378K321

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

98.588

100

0.986
  comM Acinetobacter nosocomialis M2

42.938

100

0.429
  pilM Acinetobacter baumannii D1279779

42.655

100

0.427
  comM Acinetobacter baylyi ADP1

41.457

100

0.418

Multiple sequence alignment