Detailed information NTG0371005

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FK537_RS09325
• Genome accession: NZ_CP041291
• Coordinates: 1856221..1857279 (+)
• Length: 352 a.a.
• NCBI ID: WP_005180518.1
• Uniprot ID: N9M7K9
• Organism: Acinetobacter indicus strain 94-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1851221..1862279

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FK537_RS09310 (FK537_09380)	mraY	1851363..1852481 (+)	1119	WP_005180530.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-
FK537_RS09315 (FK537_09385)	-	1852547..1853350 (+)	804	WP_104483911.1	putative RNA methyltransferase	-
FK537_RS09320 (FK537_09390)	ponA	1853473..1856049 (-)	2577	WP_104470267.1	penicillin-binding protein PBP1a	-
FK537_RS09325 (FK537_09395)	pilM	1856221..1857279 (+)	1059	WP_005180518.1	pilus assembly protein PilM	Machinery gene
FK537_RS09330 (FK537_09400)	pilN	1857279..1857914 (+)	636	WP_005180516.1	PilN domain-containing protein	Machinery gene
FK537_RS09335 (FK537_09405)	pilO	1857911..1858630 (+)	720	WP_005180513.1	type 4a pilus biogenesis protein PilO	Machinery gene
FK537_RS09340 (FK537_09410)	pilP	1858630..1859157 (+)	528	WP_168370223.1	pilus assembly protein PilP	Machinery gene
FK537_RS09345 (FK537_09415)	pilQ	1859218..1861356 (+)	2139	WP_168370224.1	type IV pilus secretin PilQ	Machinery gene
FK537_RS09350 (FK537_09420)	aroK	1861393..1861935 (+)	543	WP_075167858.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38967.63 Da
• Isoelectric Point: 4.5587

>NTDB_id=371005 FK537_RS09325 WP_005180518.1 1856221..1857279(+) (pilM) [Acinetobacter indicus strain 94-2]
MRRLYRKPSKGLIGVDISSTSVKLLELSVKNDRYWVESYALVPLPEGSVVEKNILNPEAVGDALERAFNLANTPTTAAAL
AIPTSMVISKTIEMDADMTDDEREVQIRMDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRLENVESRCEAIEL
AGLTPKVADVESFAIENAFKVFADTLPMGVNTIGVLDIGHTMTTLSVLKDNKIIYTREQVFGGKQLTQEIQNRYGLSFEE
AGRAKKSRTLPDDYDVEVLEPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSSKIDIKRIESDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=371005 FK537_RS09325 WP_005180518.1 1856221..1857279(+) (pilM) [Acinetobacter indicus strain 94-2]
GTGCGCAGGTTATATCGTAAACCGAGTAAGGGGTTAATAGGTGTAGATATTAGTTCTACTTCTGTTAAGTTATTGGAGCT
CTCTGTAAAGAATGATCGTTACTGGGTGGAAAGTTACGCCTTGGTACCGCTCCCAGAGGGCAGTGTGGTTGAGAAAAATA
TTCTGAATCCTGAAGCTGTAGGGGATGCGCTCGAGCGTGCCTTTAATTTGGCCAATACACCGACAACAGCTGCCGCTTTA
GCGATTCCAACCTCAATGGTAATTAGCAAGACCATTGAGATGGATGCCGATATGACGGATGACGAACGTGAAGTTCAAAT
CCGGATGGATGCAGAGCAATATATTCCTTTCCCATTAGATGAAGTTAGCCTGGACTTTGAGGTGCTGCCTGATCGTCTGG
CCAATCCAAACCGGGTCAATGTTTTGCTGGTGGCGACACGACTGGAAAATGTCGAGAGTCGCTGTGAAGCGATTGAACTG
GCCGGTTTAACCCCAAAAGTGGCAGATGTCGAAAGCTTTGCCATTGAAAATGCCTTTAAAGTCTTCGCCGATACCTTGCC
GATGGGGGTAAATACCATTGGTGTGTTAGACATTGGTCATACTATGACCACGCTGTCGGTACTCAAAGACAATAAAATTA
TCTATACCCGCGAGCAGGTCTTTGGTGGCAAGCAGCTGACCCAGGAAATCCAGAACCGTTATGGTCTGTCCTTTGAAGAA
GCCGGCCGCGCCAAGAAAAGCCGTACCTTGCCAGATGATTATGATGTTGAAGTGTTGGAGCCATTCCTTGAGGCGGTGGT
GCAGCAGGCGGCACGTTCGCTGCAGTTCTTCTTCTCCTCATCGCAGTTTAATGAAATTGACCATATTCTGCTGGCCGGTG
GGAATGCCAATATTCCAGGCCTGGCCAAGCTGCTCCAGCAAAAACTGGGTTACCGCGTCACCATTGCCAATCCGTTTTTA
CAGATGGGTTTCTCCTCCAAAATTGATATTAAAAGAATTGAAAGCGATGCCTCTTCATTGATGGTGGCATGTGGCTTGGC
ATTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9M7K9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	84.375	100	0.844
comM	Acinetobacter nosocomialis M2	84.375	100	0.844
comM	Acinetobacter baylyi ADP1	78.693	100	0.787
pilM	Legionella pneumophila strain ERS1305867	41.243	100	0.415