Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FK537_RS06520 Genome accession   NZ_CP041291
Coordinates   1269287..1270993 (+) Length   568 a.a.
NCBI ID   WP_075167609.1    Uniprot ID   A0AAW8Z0C9
Organism   Acinetobacter indicus strain 94-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1264287..1275993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FK537_RS06480 (FK537_06555) nusA 1264954..1266438 (-) 1485 WP_005181786.1 transcription termination factor NusA -
  FK537_RS06485 (FK537_06560) rimP 1266471..1266995 (-) 525 WP_005181784.1 ribosome maturation factor RimP -
  FK537_RS06510 (FK537_06585) secG 1267864..1268193 (-) 330 WP_168369781.1 preprotein translocase subunit SecG -
  FK537_RS06515 (FK537_06590) tpiA 1268207..1268998 (-) 792 WP_005181780.1 triose-phosphate isomerase -
  FK537_RS06520 (FK537_06595) pilB 1269287..1270993 (+) 1707 WP_075167609.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FK537_RS06525 (FK537_06600) pilC 1271024..1272250 (+) 1227 WP_016659656.1 type II secretion system F family protein Machinery gene
  FK537_RS06530 (FK537_06605) pilD 1272250..1273110 (+) 861 WP_075175010.1 prepilin peptidase Machinery gene
  FK537_RS06535 (FK537_06610) coaE 1273121..1273729 (+) 609 WP_075167608.1 dephospho-CoA kinase -
  FK537_RS06540 (FK537_06615) - 1273726..1274646 (-) 921 WP_016659657.1 DMT family transporter -
  FK537_RS06545 (FK537_06620) rlmB 1274664..1275416 (-) 753 WP_005181769.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FK537_RS06550 (FK537_06625) - 1275522..1275845 (-) 324 WP_168369782.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63787.16 Da        Isoelectric Point: 4.8301

>NTDB_id=370992 FK537_RS06520 WP_075167609.1 1269287..1270993(+) (pilB) [Acinetobacter indicus strain 94-2]
MSVQQAMPRFTGFIRRLVEEGLVTAENMQQAIVAARKAETDIVPYLIQQLQISPLTIAEKISYEFGEPIFDLAVYDSRLI
VREGLDEKLFTKYRILPIFRRHNQLYIATSNPTNIEAIDAIRFNSKLNIEVVIVEHDKLERLIEQNFTEDSTFDFDEDFD
LEVGDNAPETQEDDDEPQGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRIDGVLRQIANPPLQLATRLASRL
KVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKIVLRILDPSSAMLGIDALGYEPEQKELFMEALDKPQG
MLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIVMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCQKCKAPVEIPRQSLLELGFTE
EDLNQPEFQLYQPVGCPECREGYKGRVGVYEVMKVTPEISRIIMEDGNALQIAAASAKSGFNNLRRSGLIKVMQGVTSLQ
EVNRVTSE

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=370992 FK537_RS06520 WP_075167609.1 1269287..1270993(+) (pilB) [Acinetobacter indicus strain 94-2]
ATGTCAGTACAACAAGCAATGCCTAGATTTACAGGTTTTATTCGGCGACTGGTCGAAGAAGGGCTTGTGACGGCTGAAAA
TATGCAGCAAGCCATTGTCGCGGCAAGAAAAGCCGAGACCGATATTGTGCCGTATTTAATTCAGCAGTTGCAGATTTCAC
CGCTGACTATTGCTGAAAAAATTTCCTATGAATTTGGTGAGCCGATTTTTGACTTAGCCGTGTACGATTCGCGTCTGATT
GTACGTGAAGGTCTGGATGAAAAACTGTTCACCAAATACCGGATTCTGCCCATTTTCCGACGTCACAATCAGCTTTATAT
TGCTACCAGTAACCCAACCAATATCGAAGCCATTGATGCGATCCGCTTTAACAGCAAGCTGAATATTGAAGTGGTGATTG
TTGAGCATGACAAGCTGGAACGGCTGATTGAACAGAACTTTACCGAAGACAGCACCTTTGACTTTGATGAAGACTTTGAT
CTAGAAGTTGGCGACAACGCTCCCGAAACACAAGAAGATGACGATGAGCCACAAGGCGATGAAGCCCCAATCGTCAAATA
TATTAACAAATTACTAGTCGATGCGATCCGCATGGGCGCCTCGGATTTGCACTTCGAGCCTTATGAAAAAAGCTACCGGG
TCCGTTACCGGATTGACGGCGTACTGCGCCAGATTGCCAATCCTCCGTTACAGCTGGCCACACGCCTCGCCTCACGTTTA
AAAGTTATGTCACAAATGGACATCTCAGAAAAACGTATTCCGCAGGATGGCCGGATCAAGCTCAAGCTGTCAAAAAACAA
AGCCATCGACTTCCGTGTCAACTCACTACCCACCCTGTTTGGAGAAAAAATCGTACTACGTATTCTCGATCCATCTAGCG
CCATGCTGGGGATTGATGCACTTGGTTATGAACCGGAGCAAAAAGAGCTGTTTATGGAGGCGCTGGACAAACCACAAGGC
ATGTTACTGATTACCGGTCCGACCGGTTCCGGTAAAACCGTGTCTTTATATACCGGCTTAAATATCCTCAACCGCGAAGA
CACCAATATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGGAAGGGATTAATCAGGTCAACGTCAATCCGAAAGTGG
GTCTGACCTTTGCCGCAGCACTAAAATCATTCCTGCGCCAGGATCCGGATATTGTCATGGTCGGTGAGATCCGTGATCTG
GAAACTGCAGAAATTGCTATTAAAGCTGCACAAACCGGTCACATGGTCATGTCGACGCTGCACACCAACAGTGCACCTGA
AACCCTGACCCGTTTACGCAACATGGGCGTCCCTTCATTTAACATCGCCACCTCGGTGAACCTGGTGATTGCCCAGCGTC
TGGCACGTCGTCTGTGTCAGAAATGCAAGGCCCCGGTGGAGATTCCACGCCAGAGCCTCTTGGAGCTCGGCTTTACCGAA
GAAGATTTAAACCAGCCAGAGTTCCAGCTGTATCAGCCGGTTGGCTGCCCGGAATGTCGTGAAGGTTATAAAGGCCGTGT
CGGGGTCTATGAAGTGATGAAAGTCACCCCGGAAATCTCACGCATTATTATGGAAGATGGCAATGCCTTGCAGATTGCAG
CAGCCTCGGCAAAATCCGGGTTTAACAATTTACGCCGTTCAGGATTAATCAAGGTCATGCAGGGGGTCACTTCTTTACAG
GAAGTGAATCGTGTGACCAGCGAATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

80.351

100

0.806
  pilB Acinetobacter baylyi ADP1

77.895

100

0.782
  pilB Legionella pneumophila strain ERS1305867

50.97

99.824

0.509
  pilF Neisseria gonorrhoeae MS11

51.429

98.592

0.507
  pilB Vibrio cholerae strain A1552

49.647

99.648

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

49.372

98.063

0.484
  pilB Vibrio campbellii strain DS40M4

50.864

91.725

0.467
  pilF Thermus thermophilus HB27

37.434

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.364

Multiple sequence alignment