Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FJ709_RS01470 Genome accession   NZ_CP041216
Coordinates   348581..350293 (-) Length   570 a.a.
NCBI ID   WP_226412767.1    Uniprot ID   -
Organism   Shewanella glacialimarina strain TZS-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343581..355293
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJ709_RS01440 (FJ709_01440) mutT 344153..344551 (-) 399 WP_226412755.1 8-oxo-dGTP diphosphatase MutT -
  FJ709_RS01445 (FJ709_01445) yacG 344582..344809 (-) 228 WP_226412757.1 DNA gyrase inhibitor YacG -
  FJ709_RS01450 (FJ709_01450) zapD 344863..345597 (-) 735 WP_226412759.1 cell division protein ZapD -
  FJ709_RS01455 (FJ709_01455) coaE 345590..346207 (-) 618 WP_226412761.1 dephospho-CoA kinase -
  FJ709_RS01460 (FJ709_01460) pilD 346235..347164 (-) 930 WP_226412763.1 A24 family peptidase Machinery gene
  FJ709_RS01465 (FJ709_01465) pilC 347215..348483 (-) 1269 WP_226412765.1 type II secretion system F family protein Machinery gene
  FJ709_RS01470 (FJ709_01470) pilB 348581..350293 (-) 1713 WP_226412767.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FJ709_RS01475 (FJ709_01475) - 350382..350801 (-) 420 WP_319002888.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FJ709_RS01480 (FJ709_01480) nadC 351613..352491 (-) 879 WP_226412769.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FJ709_RS01485 (FJ709_01485) ampD 352775..353356 (+) 582 WP_226412771.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FJ709_RS01490 (FJ709_01490) ampE 353446..354297 (+) 852 WP_226412773.1 beta-lactamase regulator AmpE -
  FJ709_RS01495 (FJ709_01495) pdhR 354502..355254 (+) 753 WP_226412775.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63085.18 Da        Isoelectric Point: 5.4412

>NTDB_id=370604 FJ709_RS01470 WP_226412767.1 348581..350293(-) (pilB) [Shewanella glacialimarina strain TZS-4]
MPNTALNLGLSTLFIRKNLLTEEQLSTAISHSRKNRSSLVSALITNKFLSAREIAELCYEEYGTPLLDLNEFDITNIAEE
LLNKKLISKHKCLPLFKRGNRLYIATSDPTNISALEDFQFSLGLHAEAILVEDDKLHKVLEKVLEEDLNSLGLDGLDEEA
LAGLDVTDTNQHEDEPSADSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPVNLSARISA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSSLPTIWGEKIVMRILDSSSAQLGIEKLGYEDDQRLLYEEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQPEDVPAAELLRLGF
NQQNIDAGFTVYKPCGCDLCSGGYKGRVGIYEVMKISDAIARTIMESGNSLQIAQQAKSEGMRDLRQSGLLKVVHGVTSI
SEVNRVTSFN

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=370604 FJ709_RS01470 WP_226412767.1 348581..350293(-) (pilB) [Shewanella glacialimarina strain TZS-4]
ATGCCTAATACAGCGTTAAATTTAGGATTATCGACATTATTTATCCGCAAGAACCTCTTAACTGAGGAGCAGTTGTCGAC
GGCCATTTCCCACTCAAGAAAAAACAGATCCTCTTTAGTCAGTGCATTAATTACCAATAAGTTTTTGTCTGCACGGGAAA
TTGCTGAGCTTTGCTATGAAGAATATGGCACACCGCTACTTGATCTCAATGAATTTGATATCACCAATATTGCCGAAGAG
TTACTCAATAAAAAGCTCATCTCCAAGCACAAATGTTTGCCACTGTTTAAGCGTGGTAACAGACTTTATATCGCAACATC
TGACCCAACCAACATTTCAGCATTAGAAGATTTTCAATTTAGTCTAGGCCTGCATGCTGAAGCCATTCTGGTGGAAGATG
ATAAACTGCATAAAGTGTTAGAAAAAGTACTAGAAGAAGATTTAAACAGCTTAGGCCTTGATGGCCTTGACGAAGAAGCC
TTAGCAGGCTTAGATGTAACCGATACCAATCAACATGAAGATGAACCATCCGCTGACAGTAGCGACGATGCACCTATCGT
CATTTATATCAATAAAATTCTGACCGATGCCATTCGAAAAGGTGCATCAGACTTACACTTTGAACCCTATGAAAAGCGAT
ACCGGATTCGTTTTCGCATAGACGGTATTTTACATGAAGTGTCTGAACCACCTGTTAACTTATCAGCCAGAATTTCAGCC
CGTTTAAAAGTAATGTCAAAGTTGGATATTGCCGAGCGCCGCGTGCCGCAAGATGGCCGGATTAAAATGAAACTGTCACG
CACAAAATCCATTGATTTTCGTGTCAGTAGCTTGCCCACAATCTGGGGCGAAAAAATTGTAATGCGTATTTTGGATTCAT
CTTCAGCGCAATTAGGCATTGAAAAGCTCGGTTATGAAGATGACCAACGTTTATTGTATGAAGAAATGCTCGCCAAACCC
CAGGGGATGATTTTAGTCACTGGCCCGACGGGTTCAGGTAAAACCGTATCCTTATATACTGGCCTAAATATATTAAACAC
CGAAGAGCGCAATATTTCTACCGCCGAAGACCCGGTAGAAATTAACCTAGAAGGGGTTAACCAGGTTCATATTAATTTAA
AAGCAGGCTTGACCTTTGCATCAGCACTACGATCATTTTTACGCCAAGACCCTGACGTGGTGATGGTAGGTGAAATACGT
GATTTAGAAACAGCCGAAATTGCCATTAAGGCCGCGCAAACCGGTCATTTAGTATTATCAACATTACATACTAACTCGGC
AGCAGAAACCCTTACCCGTTTAATTAACATGGGTGTACCCGGCTATAACATTGCCAGCTCAGTGAATTTAATTATTGCCC
AGCGCTTAGCCAGACGTTTATGCCCCGAGTGCAAGCAGCCTGAAGATGTACCCGCTGCAGAATTGTTGCGCCTTGGTTTT
AACCAACAAAACATTGATGCAGGGTTTACCGTTTATAAACCCTGTGGCTGTGATTTATGTTCCGGCGGCTACAAAGGCCG
TGTCGGCATTTATGAAGTAATGAAAATATCCGATGCCATCGCGCGTACGATTATGGAAAGTGGTAACTCTTTGCAAATTG
CCCAGCAAGCAAAATCCGAGGGTATGCGAGATTTGCGTCAATCCGGGTTACTGAAAGTAGTCCATGGCGTTACTAGTATT
TCTGAAGTCAATCGTGTCACCAGTTTTAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.511

98.947

0.579
  pilB Acinetobacter baylyi ADP1

57.932

98.421

0.57
  pilB Legionella pneumophila strain ERS1305867

55.95

98.772

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

52.575

98.772

0.519
  pilB Vibrio cholerae strain A1552

51.865

98.772

0.512
  pilB Vibrio campbellii strain DS40M4

51.332

98.772

0.507
  pilF Neisseria gonorrhoeae MS11

46.975

98.596

0.463
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.657

96.667

0.374
  pilF Thermus thermophilus HB27

37.722

98.596

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

40.232

90.702

0.365

Multiple sequence alignment