Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3M66_RS19440 Genome accession   NZ_CP048609
Coordinates   4017012..4018397 (+) Length   461 a.a.
NCBI ID   WP_001025186.1    Uniprot ID   -
Organism   Escherichia coli strain STEFF_1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4012012..4023397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M66_RS19415 (G3M66_19205) aroP 4012560..4013933 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  G3M66_RS19420 (G3M66_19210) ampE 4013976..4014830 (-) 855 WP_000172009.1 beta-lactamase regulator AmpE -
  G3M66_RS19425 (G3M66_19215) ampD 4014827..4015378 (-) 552 WP_000923725.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G3M66_RS19430 (G3M66_19220) nadC 4015466..4016359 (+) 894 WP_001135147.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G3M66_RS19435 (G3M66_19225) pilA 4016562..4017002 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  G3M66_RS19440 (G3M66_19230) pilB 4017012..4018397 (+) 1386 WP_001025186.1 type II secretion system protein GspE Machinery gene
  G3M66_RS19445 (G3M66_19235) hofC 4018387..4019589 (+) 1203 WP_000157222.1 protein transport protein HofC -
  G3M66_RS19450 (G3M66_19240) guaC 4019624..4020667 (-) 1044 WP_001217337.1 GMP reductase -
  G3M66_RS19455 - 4020823..4020867 (-) 45 WP_120795372.1 protein YacM -
  G3M66_RS19460 (G3M66_19245) coaE 4020892..4021512 (+) 621 WP_001269506.1 dephospho-CoA kinase -
  G3M66_RS19465 (G3M66_19250) zapD 4021512..4022255 (+) 744 WP_001194731.1 cell division protein ZapD -
  G3M66_RS19470 (G3M66_19255) yacG 4022265..4022462 (+) 198 WP_000005042.1 DNA gyrase inhibitor YacG -
  G3M66_RS19475 (G3M66_19260) mutT 4022551..4022949 (-) 399 WP_000736040.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50638.28 Da        Isoelectric Point: 6.5396

>NTDB_id=370059 G3M66_RS19440 WP_001025186.1 4017012..4018397(+) (pilB) [Escherichia coli strain STEFF_1]
MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFATTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQP
KAELLTRTLQSALEQRASDIHIEPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPSQLVDFAHALQQPQGLVLVTGPTGSGKTVTLYSALQTLN
TADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNS
TCETLVRLQQMGVARWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRTALFE
VLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRVLGMPHGE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=370059 G3M66_RS19440 WP_001025186.1 4017012..4018397(+) (pilB) [Escherichia coli strain STEFF_1]
ATGAATATTCCACAGCTCACCGCCCTGTGTCTGCGTTATCAGGGAGTCTTGCTGGATGCCAGCGAAGAGGTGGTTCATGT
TGCGGTAGTCGATGCACCTTCGCATGAGCTACTGGACGCATTGCATTTCGCTACCACCAAACGTATTGAGATCACCTGCT
GGACGCGCCAACAAATGGAAGGTCACGCCAGTCGCACACAACAGACATTGCCCGTAGCTGTTCAGGAGAAGCATCAGCCC
AAAGCAGAGTTGCTGACTCGAACGTTACAGTCTGCGCTGGAACAACGCGCGTCTGATATTCATATCGAACCAGCGGACAA
TGCCTACCGCATCCGCTTGCGTATCGACGGCGTATTGCATCCTTTACCGGACGTTTCACCGGATGCCGGAGTCGCATTAA
CCGCCAGATTAAAAGTGCTGGGAAATCTGGATATTGCGGAACATCGCCTGCCGCAGGACGGGCAATTCACTGTCGAACTG
GCAGGAAACGCCGTCTCATTTCGTATTGCGACCTTACCATGTCGGGGTGGTGAAAAGGTGGTATTAAGGTTGTTACAGCA
GGTGAACCAGGCACTGGATGTTAATACGCTTGGAATGCAGCCGTCACAACTGGTGGACTTTGCTCATGCCTTGCAACAAC
CACAGGGACTGGTGCTGGTAACTGGCCCTACAGGCAGCGGCAAAACGGTCACGCTTTATAGTGCCCTGCAAACACTGAAT
ACCGCTGACATTAATATTTGTAGCGTCGAAGATCCGGTTGAGATCCCCATAGCCGGACTAAACCAGACGCAAATCCATCC
GCGTGCCGGACTCACCTTTCAGGGCGTTTTGCGTGCGTTATTGCGCCAGGACCCTGACGTCATCATGATCGGAGAGATCC
GCGATGGCGAAACGGCAGAAATTGCCATTAAAGCCGCGCAAACCGGTCACCTGGTGTTGTCTACCCTACACACTAATTCC
ACCTGCGAAACGCTGGTACGTTTACAGCAAATGGGAGTCGCCCGCTGGATGCTATCATCGGCGCTTACGCTGGTAATAGC
CCAGCGTCTGGTACGCAAACTTTGCCCGCATTGTCGCCGGCAGCAAGGGGAGCCCATCCATATTCCAGACAATGTATGGC
CATCGCCGCTGCCCCACTGGCAGGCACCCGGTTGTGTACATTGCTACCACGGTTTTTATGGTCGCACGGCCTTATTTGAA
GTTCTGCCCATAACACCGGTCATACGTCAGCTTATTTCCGCTAATACCGACGTTGAATCGCTGGAAACGCACGCCCGACA
GGCGGGTATGCGAACGCTTTTTGAAAACGGCTGCCTGGCCGTGGAGCAAGGCTTAACCACCTTTGAAGAGTTAATCCGCG
TATTGGGGATGCCGCATGGCGAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(88-353)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

50

83.297

0.416
  pilB Glaesserella parasuis strain SC1401

41.253

100

0.414
  pilB Vibrio campbellii strain DS40M4

48.446

83.731

0.406
  pilB Acinetobacter baylyi ADP1

39.744

100

0.403
  pilB Vibrio cholerae strain A1552

46.667

84.599

0.395
  pilB Vibrio parahaemolyticus RIMD 2210633

46.154

84.599

0.39
  pilB Haemophilus influenzae 86-028NP

45.22

83.948

0.38
  pilB Haemophilus influenzae Rd KW20

44.444

83.948

0.373
  pilF Neisseria gonorrhoeae MS11

44.416

83.514

0.371
  pilB Acinetobacter baumannii D1279779

43.401

85.466

0.371