Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   G3M66_RS19435 Genome accession   NZ_CP048609
Coordinates   4016562..4017002 (+) Length   146 a.a.
NCBI ID   WP_000360914.1    Uniprot ID   A0A0H2YVZ3
Organism   Escherichia coli strain STEFF_1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4011562..4022002
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M66_RS19415 (G3M66_19205) aroP 4012560..4013933 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  G3M66_RS19420 (G3M66_19210) ampE 4013976..4014830 (-) 855 WP_000172009.1 beta-lactamase regulator AmpE -
  G3M66_RS19425 (G3M66_19215) ampD 4014827..4015378 (-) 552 WP_000923725.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  G3M66_RS19430 (G3M66_19220) nadC 4015466..4016359 (+) 894 WP_001135147.1 carboxylating nicotinate-nucleotide diphosphorylase -
  G3M66_RS19435 (G3M66_19225) pilA 4016562..4017002 (+) 441 WP_000360914.1 prepilin peptidase-dependent pilin Machinery gene
  G3M66_RS19440 (G3M66_19230) pilB 4017012..4018397 (+) 1386 WP_001025186.1 type II secretion system protein GspE Machinery gene
  G3M66_RS19445 (G3M66_19235) hofC 4018387..4019589 (+) 1203 WP_000157222.1 protein transport protein HofC -
  G3M66_RS19450 (G3M66_19240) guaC 4019624..4020667 (-) 1044 WP_001217337.1 GMP reductase -
  G3M66_RS19455 - 4020823..4020867 (-) 45 WP_120795372.1 protein YacM -
  G3M66_RS19460 (G3M66_19245) coaE 4020892..4021512 (+) 621 WP_001269506.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15620.77 Da        Isoelectric Point: 4.5237

>NTDB_id=370058 G3M66_RS19435 WP_000360914.1 4016562..4017002(+) (pilA) [Escherichia coli strain STEFF_1]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=370058 G3M66_RS19435 WP_000360914.1 4016562..4017002(+) (pilA) [Escherichia coli strain STEFF_1]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAAATACCTGCGACGGTGGCAGCAATGGCATTCCTTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACGCCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2YVZ3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363