Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FJQ87_RS03640 Genome accession   NZ_CP041151
Coordinates   806602..808308 (-) Length   568 a.a.
NCBI ID   WP_140930627.1    Uniprot ID   A0A4Y6I8A8
Organism   Shewanella sp. SNU WT4 strain SNU WT1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 801602..813308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FJQ87_RS03610 (FJQ87_03610) mutT 801995..802396 (-) 402 WP_140930615.1 8-oxo-dGTP diphosphatase MutT -
  FJQ87_RS03615 (FJQ87_03615) yacG 802393..802611 (-) 219 WP_140930617.1 DNA gyrase inhibitor YacG -
  FJQ87_RS03620 (FJQ87_03620) zapD 802738..803469 (-) 732 WP_140930619.1 cell division protein ZapD -
  FJQ87_RS03625 (FJQ87_03625) coaE 803477..804082 (-) 606 WP_140930621.1 dephospho-CoA kinase -
  FJQ87_RS03630 (FJQ87_03630) pilD 804090..805019 (-) 930 WP_140930623.1 A24 family peptidase Machinery gene
  FJQ87_RS03635 (FJQ87_03635) pilC 805280..806542 (-) 1263 WP_140930625.1 type II secretion system F family protein Machinery gene
  FJQ87_RS03640 (FJQ87_03640) pilB 806602..808308 (-) 1707 WP_140930627.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FJQ87_RS18910 (FJQ87_03645) - 808324..808746 (-) 423 WP_140930629.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FJQ87_RS03650 (FJQ87_03650) nadC 809576..810454 (-) 879 WP_140930631.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FJQ87_RS03655 (FJQ87_03655) ampD 810689..811303 (+) 615 WP_140930633.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FJQ87_RS03660 (FJQ87_03660) ampE 811386..812279 (+) 894 WP_140930635.1 beta-lactamase regulator AmpE -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63015.32 Da        Isoelectric Point: 5.5579

>NTDB_id=369921 FJQ87_RS03640 WP_140930627.1 806602..808308(-) (pilB) [Shewanella sp. SNU WT4 strain SNU WT1]
MSTTSLHLGLSTLFIKQGLLTTEQLNAAIGQSKSRRQSLVATLVADNIISAKTISELCYQEYGTPLLDLAEFDLSLIPEK
FLNKKLIEKHRCLPLFERGKRLYIATSDPTNFNALEDFQFSAGLHAEPILVEEDKLAKALEKVLEDDLSSLDFGNMDESA
LADMEVQDTSNNLDDIKDEADEAPIVMYINKILTDAIRKGASDLHFEPYEKRYRVRFRIDGILHQVSEPPINLSNRISAR
LKVMAKLDIAERRVPQDGRIKMKLSRTKSIDFRVSSLPTLWGEKIVMRILDSSSAQLGIEKLGYEPEQQALYMEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSASETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPHCREPEDVPQHELLRLGFS
EEQIGHGFTLYKPVGCDACAGGYKGRVGIYETLKMSDEISRIIMEGGNSLQINDVAKQQGMNDLRQSGLLKVIQGMTSLA
EINRVTSF

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=369921 FJQ87_RS03640 WP_140930627.1 806602..808308(-) (pilB) [Shewanella sp. SNU WT4 strain SNU WT1]
ATGTCTACCACTAGCCTACATTTAGGGTTATCGACCCTCTTCATTAAACAAGGGCTGCTCACCACTGAGCAGCTAAATGC
AGCTATTGGTCAGTCAAAAAGCCGCCGCCAGAGCTTAGTTGCGACTCTGGTGGCGGATAATATTATCTCCGCTAAAACCA
TTTCTGAGCTTTGCTATCAAGAATACGGCACGCCGCTGCTTGATTTAGCCGAGTTTGATTTAAGCTTAATTCCAGAAAAA
TTTCTTAATAAAAAACTGATTGAAAAGCACCGCTGCCTGCCGCTGTTTGAGCGCGGTAAACGCCTGTATATCGCCACCTC
TGATCCCACTAATTTTAATGCCCTCGAAGACTTTCAATTTAGTGCCGGCCTGCACGCAGAACCCATATTAGTAGAAGAAG
ATAAGCTCGCTAAAGCCCTTGAAAAGGTGCTTGAAGATGATTTATCTAGCCTAGATTTTGGCAATATGGATGAGTCGGCC
TTGGCCGATATGGAAGTGCAAGATACCAGTAACAACCTTGATGATATTAAAGATGAGGCCGATGAAGCGCCTATCGTCAT
GTATATCAATAAGATTCTGACCGATGCCATTCGAAAAGGGGCGTCCGATTTACATTTTGAGCCTTATGAAAAACGCTACC
GCGTGCGCTTTCGTATTGATGGTATCTTGCATCAAGTATCAGAACCGCCGATTAACTTAAGCAATCGAATATCGGCGCGC
TTAAAGGTCATGGCCAAACTTGATATTGCTGAGCGCCGAGTGCCACAAGATGGCCGCATTAAAATGAAATTATCGCGCAC
CAAAAGCATAGATTTTCGAGTATCTAGCCTCCCAACCCTGTGGGGGGAAAAAATCGTAATGCGTATTTTAGACTCATCTT
CTGCGCAGCTTGGTATTGAAAAACTCGGTTATGAGCCCGAGCAACAAGCCTTGTATATGGAGATGCTAGCCAAACCTCAG
GGCATGATTTTAGTCACAGGCCCCACGGGCTCAGGCAAAACCGTATCCCTTTATACTGGCCTTAATATTCTTAATACCAC
AGAGCGCAATATTTCTACCGCCGAAGATCCGGTAGAAATAAACTTAGAAGGCGTCAATCAAGTTCACATTAATTTAAAGG
CGGGGCTAACCTTTGCGTCCGCCTTGCGCTCATTCTTGCGTCAAGACCCAGATGTGGTGATGGTGGGTGAAATCCGTGAC
TTAGAAACCGCTGAAATTGCCATTAAAGCGGCGCAAACCGGTCACTTAGTATTAAGCACCTTACATACCAACTCGGCTTC
CGAAACCTTAACCCGCTTAATCAATATGGGCGTGCCGGGTTACAACATAGCAAGTTCTGTCAATTTAATTATTGCCCAGC
GCTTAGCCCGTAGGCTCTGCCCCCATTGCCGCGAGCCAGAAGATGTACCCCAACATGAATTGCTGCGTTTAGGCTTTAGT
GAAGAGCAAATTGGCCACGGTTTTACCCTCTATAAGCCAGTGGGCTGCGATGCTTGCGCTGGCGGTTACAAAGGCCGAGT
AGGTATTTATGAAACCCTAAAGATGAGTGATGAAATATCCCGCATCATTATGGAGGGTGGTAATTCCCTACAAATCAATG
ATGTTGCCAAGCAGCAAGGCATGAATGATTTGCGCCAATCTGGATTATTAAAAGTGATTCAAGGCATGACCAGCTTGGCA
GAAATTAACCGCGTCACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y6I8A8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.609

98.944

0.59
  pilB Acinetobacter baumannii D1279779

57.996

100

0.581
  pilB Legionella pneumophila strain ERS1305867

55.844

94.894

0.53
  pilB Vibrio parahaemolyticus RIMD 2210633

51.056

100

0.511
  pilB Vibrio campbellii strain DS40M4

51.15

99.472

0.509
  pilB Vibrio cholerae strain A1552

50.896

98.239

0.5
  pilF Neisseria gonorrhoeae MS11

47.611

99.472

0.474
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.23

99.472

0.38
  pilF Thermus thermophilus HB27

37.746

98.415

0.371

Multiple sequence alignment