Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   G3M71_RS03765 Genome accession   NZ_CP048603
Coordinates   789621..791114 (+) Length   497 a.a.
NCBI ID   WP_163388414.1    Uniprot ID   -
Organism   Escherichia coli strain ChlosBP-23-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 784621..796114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M71_RS03745 (G3M71_03745) pilD 785269..786078 (+) 810 WP_163388412.1 prepilin peptidase PppA Machinery gene
  G3M71_RS03750 (G3M71_03750) gspS2 786144..786554 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  G3M71_RS03755 (G3M71_03755) gspC 786572..787531 (+) 960 WP_163388413.1 type II secretion system protein GspC -
  G3M71_RS03760 (G3M71_03760) gspD 787561..789621 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  G3M71_RS03765 (G3M71_03765) pilB/pilB1 789621..791114 (+) 1494 WP_163388414.1 type II secretion system ATPase GspE Machinery gene
  G3M71_RS03770 (G3M71_03770) gspF 791114..792337 (+) 1224 WP_083576245.1 type II secretion system inner membrane protein GspF -
  G3M71_RS03775 (G3M71_03775) gspG 792354..792809 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  G3M71_RS03780 (G3M71_03780) gspH 792813..793376 (+) 564 WP_163388415.1 type II secretion system minor pseudopilin GspH -
  G3M71_RS03785 (G3M71_03785) gspI 793373..793744 (+) 372 WP_000820090.1 type II secretion system minor pseudopilin GspI -
  G3M71_RS03790 (G3M71_03790) gspJ 793741..794346 (+) 606 WP_163388416.1 type II secretion system minor pseudopilin GspJ -
  G3M71_RS03795 (G3M71_03795) gspK 794343..795320 (+) 978 WP_163388417.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55204.31 Da        Isoelectric Point: 6.0089

>NTDB_id=369773 G3M71_RS03765 WP_163388414.1 789621..791114(+) (pilB/pilB1) [Escherichia coli strain ChlosBP-23-1]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=369773 G3M71_RS03765 WP_163388414.1 789621..791114(+) (pilB/pilB1) [Escherichia coli strain ChlosBP-23-1]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGTTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGCGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGACCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACGGGGCACCT
GGTACTTTCCACACTGCATACCAACACGGCGGTGGGGGCGATTACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGTGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCACCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCGCGTACCGTCACTCTGTACCATCCACAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGTTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment