Detailed information NTG0369056

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FIM01_RS15745
• Genome accession: NZ_CP041035
• Coordinates: 3183674..3184792 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 11W359501
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3178674..3189792

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FIM01_RS15735 (FIM01_15735)	-	3181790..3182482 (-)	693	WP_001108506.1	YggS family pyridoxal phosphate-dependent enzyme	-
FIM01_RS15740 (FIM01_15740)	pilT	3182609..3183646 (+)	1038	WP_000355491.1	type IV pilus twitching motility protein PilT	Machinery gene
FIM01_RS15745 (FIM01_15745)	pilU	3183674..3184792 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FIM01_RS15750 (FIM01_15750)	fur	3184862..3185299 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
FIM01_RS15755 (FIM01_15755)	-	3185411..3185809 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
FIM01_RS15760 (FIM01_15760)	-	3185864..3186187 (-)	324	WP_000442585.1	RnfH family protein	-
FIM01_RS15765 (FIM01_15765)	-	3186187..3187254 (-)	1068	WP_000092403.1	hypothetical protein	-
FIM01_RS15775 (FIM01_15775)	-	3187407..3187862 (-)	456	WP_000782976.1	bacteriohemerythrin	-
FIM01_RS15780 (FIM01_15780)	-	3188115..3188963 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=369056 FIM01_RS15745 WP_000347036.1 3183674..3184792(+) (pilU) [Acinetobacter baumannii strain 11W359501]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=369056 FIM01_RS15745 WP_000347036.1 3183674..3184792(+) (pilU) [Acinetobacter baumannii strain 11W359501]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGTGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392