Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FFU37_RS13440 Genome accession   NZ_CP040558
Coordinates   3012544..3014220 (+) Length   558 a.a.
NCBI ID   WP_138489701.1    Uniprot ID   A0A4P9J395
Organism   Pseudoalteromonas distincta strain 16-SW-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3007544..3019220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFU37_RS13415 (FFU37_13415) nadC 3007617..3008468 (+) 852 WP_138489698.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FFU37_RS13420 (FFU37_13420) - 3008952..3009416 (+) 465 WP_217496773.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FFU37_RS13425 (FFU37_13425) pilC 3009492..3010718 (+) 1227 WP_138489699.1 type II secretion system F family protein Machinery gene
  FFU37_RS13430 (FFU37_13430) pilD 3010881..3011774 (+) 894 WP_138490040.1 A24 family peptidase Machinery gene
  FFU37_RS13435 (FFU37_13435) coaE 3011776..3012417 (+) 642 WP_138489700.1 dephospho-CoA kinase -
  FFU37_RS13440 (FFU37_13440) pilB 3012544..3014220 (+) 1677 WP_138489701.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FFU37_RS13445 (FFU37_13445) yacG 3014331..3014561 (+) 231 WP_002957774.1 DNA gyrase inhibitor YacG -
  FFU37_RS13450 (FFU37_13450) - 3014635..3015123 (-) 489 WP_138489702.1 Spy/CpxP family protein refolding chaperone -
  FFU37_RS13455 (FFU37_13455) - 3015313..3016002 (+) 690 WP_138489703.1 response regulator -
  FFU37_RS13460 (FFU37_13460) - 3015980..3017395 (+) 1416 WP_002957777.1 ATP-binding protein -
  FFU37_RS13465 (FFU37_13465) - 3017399..3018346 (+) 948 WP_138489704.1 alpha/beta fold hydrolase -
  FFU37_RS13470 (FFU37_13470) - 3018309..3019154 (-) 846 WP_239022731.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62349.57 Da        Isoelectric Point: 5.2406

>NTDB_id=365206 FFU37_RS13440 WP_138489701.1 3012544..3014220(+) (pilB) [Pseudoalteromonas distincta strain 16-SW-7]
MNINSPLLRKFITLGRIDADTVKNKQNEFASTAELISKCGKIDSKDLAEQCIDLFRVPYFDLKYFDPTRIPADLVKEKLI
RKHHILPLVQKDRKVYIAASDPTDYGAFENFEFSTGLSCEIVVVDYIQLDNKIEQLLDATGSLNLTDDEFQEFADLDSES
QRDTAPQDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLATKLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALAQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEQLPSEELARQGFSTEQIEEMTL
YMPKGCDSCTDGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVSLKAGFNNLRLSGLRKAADGLTSLGEINRVTNF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=365206 FFU37_RS13440 WP_138489701.1 3012544..3014220(+) (pilB) [Pseudoalteromonas distincta strain 16-SW-7]
ATGAATATTAACTCACCGTTATTGAGAAAATTTATAACCCTTGGCCGCATTGATGCCGACACGGTTAAAAACAAACAAAA
TGAATTTGCCTCAACAGCTGAGCTTATTTCTAAATGTGGAAAAATCGATAGTAAAGATTTAGCTGAACAATGTATTGATT
TGTTTCGCGTACCTTATTTTGATTTAAAATACTTTGATCCTACAAGAATCCCGGCCGATCTCGTAAAAGAAAAACTCATA
CGTAAACATCACATTTTACCGCTTGTTCAAAAAGATCGAAAAGTTTATATAGCAGCATCAGACCCAACAGATTACGGCGC
ATTCGAAAACTTTGAGTTTAGTACAGGTCTTTCATGCGAAATTGTTGTTGTTGATTATATTCAACTCGATAACAAAATTG
AGCAATTGCTAGATGCCACTGGCAGCTTAAATCTAACTGATGACGAGTTTCAAGAATTTGCTGACTTAGATTCAGAAAGC
CAAAGAGATACAGCACCACAAGATGACGATAAAGATGATGCGCCTATCATCGTATATATCAACAAAATTTTAATGGATGC
CATTAAAAAAGGCGCCTCTGATTTACACTTTGAACCGTATGAGCACAAGTACCGTATTCGCTTTCGTATTGATGGTATTT
TGCATGAAGTTGCAAGCCCACCCAATACATTAGCCACCAAGCTTTCTGCGCGTATTAAGGTTATGTCGAGACTCGATATT
GCCGAAAAACGCAAACCACAAGATGGTCGTATTAAGCTAAAAATTACTGAACGCAAAAGTATCGACTTTCGTGTAAGTAC
CATGCCTACCTTGTGGGGCGAAAAAATAGTAATGCGTATTCTTGATTCATCAAGCGCTATGTTAGGCATTGATGTATTAG
GCTATGAGCCTGAGCAGAAAAAGCTCTACATGGATGCGCTTGCCCAGCCTCAAGGGATGATACTTGTAACAGGGCCAACG
GGTTCTGGTAAAACAGTATCGCTATACACAGGTTTAAATATATTAAACCAGCCTGAGCGTAATATTAGTACTGCGGAAGA
TCCGGTTGAGATTAACCTTGAAGGTGTAAACCAAGTACAAATTAATATTAAAGCCGATATGACTTTTGCCAACGCACTGC
GCGCATTTTTACGTCAAGATCCTGATGTAGTTATGGTAGGTGAAATACGTGACTTAGAGACCGCAGAAATATCAATAAAA
GCGGCGCAAACAGGTCACTTGGTTTTATCAACATTGCATACAAACTCAGCGCCGGAAACAATCACTCGTTTATTAAATAT
GGGTGTACCCGCTTATAACGTAGCAAGTTCGATTAGCTTAATAATAGCCCAGCGTTTAGCACGTCGCTTGTGCCCTAAAT
GTAAAACGCCAGAACAACTTCCGAGTGAAGAGCTAGCTCGTCAAGGATTTAGTACCGAGCAAATTGAAGAAATGACGTTA
TATATGCCTAAAGGGTGTGATAGCTGTACTGATGGTTACAAAGGGCGTGTGGGTATTTACGAAGTAATGCAAATTACTCC
TGAAATTGCCCAAATTATTATGCGCGGTGGTAATTCGCTTGAAATAGCAGAGGTATCACTTAAAGCCGGTTTTAATAATT
TACGTTTATCGGGTTTACGCAAAGCAGCCGATGGCCTTACATCCCTTGGTGAAATTAACCGCGTAACCAATTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4P9J395

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.869

91.935

0.541
  pilB Acinetobacter baylyi ADP1

59.01

90.502

0.534
  pilB Vibrio cholerae strain A1552

52.336

95.878

0.502
  pilB Legionella pneumophila strain ERS1305867

54.42

91.219

0.496
  pilB Vibrio campbellii strain DS40M4

51.402

95.878

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

51.311

95.699

0.491
  pilF Neisseria gonorrhoeae MS11

47.491

100

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

36.495

100

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.117

99.462

0.369

Multiple sequence alignment