Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FE240_RS14145 Genome accession   NZ_CP040449
Coordinates   2982563..2984269 (+) Length   568 a.a.
NCBI ID   WP_193001826.1    Uniprot ID   A0A5J6WYY3
Organism   Aeromonas simiae strain A6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2977563..2989269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE240_RS14120 (FE240_14125) pdhR 2978876..2979637 (-) 762 WP_042047839.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FE240_RS14125 (FE240_14130) ampD 2979907..2980485 (-) 579 WP_193001819.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FE240_RS14130 (FE240_14135) - 2980509..2980991 (+) 483 WP_193001821.1 TIGR02281 family clan AA aspartic protease -
  FE240_RS14135 (FE240_14140) nadC 2980997..2981854 (+) 858 WP_193001823.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FE240_RS14140 (FE240_14145) - 2982113..2982559 (+) 447 WP_193001825.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  FE240_RS14145 (FE240_14150) pilB 2982563..2984269 (+) 1707 WP_193001826.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FE240_RS14150 (FE240_14155) - 2984305..2985543 (+) 1239 Protein_2725 type II secretion system F family protein -
  FE240_RS14155 (FE240_14160) pilD 2985562..2986434 (+) 873 WP_193001828.1 A24 family peptidase Machinery gene
  FE240_RS14160 (FE240_14165) coaE 2986447..2987061 (+) 615 WP_193001830.1 dephospho-CoA kinase -
  FE240_RS14165 (FE240_14170) zapD 2987099..2987821 (+) 723 WP_042045478.1 cell division protein ZapD -
  FE240_RS14170 (FE240_14175) yacG 2987832..2988023 (+) 192 WP_193001832.1 DNA gyrase inhibitor YacG -
  FE240_RS14175 (FE240_14180) mutT 2988073..2988477 (-) 405 WP_193001834.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62799.99 Da        Isoelectric Point: 5.8517

>NTDB_id=364563 FE240_RS14145 WP_193001826.1 2982563..2984269(+) (pilB) [Aeromonas simiae strain A6]
MTSTSNSGLALSLAASSLISEQDSQRFSTQAKAQRKPLTTFLIENQILSSRELADFCEQEYGVPLLDLDAFELGDIPPKY
INQKLIEKHHALPIYLQGHTLYIAMADPTNVAALEEFGFNFGLHTEALLVEEEKLVRAIGKLLEHDQENLNVDDIDDADI
SGLEVADEQSRLDETVNSSDDDAPIVKYINKIMMDAIRRGASDLHFEPYEHKYRIRFRIDGILHEAATPPVNLASRFAAR
LKVMARLDIAERRLPQDGRIKLKLSRNRAMDMRVNTLPTMWGEKVVIRLLDSSAARLNIDQLGFDEEQKQLYQTALARPQ
GMILVTGPTGSGKTVTLYTGLNLLNTVERNISTAEDPIEINLPGVNQVQINPKAGLTFAGALRAFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCEHCKQPDNIPEGELLKLGYS
VQQVAAGIRLYRPCGCKECSGGYRGRIGIYEILSMSDNIARIIMQGGNSLLIAAQAQKEGMRTLRQAGLEKARLGITSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=364563 FE240_RS14145 WP_193001826.1 2982563..2984269(+) (pilB) [Aeromonas simiae strain A6]
ATGACTTCAACCTCCAACAGCGGCCTGGCACTTAGCCTGGCCGCCTCTTCCCTGATCAGCGAGCAGGACTCGCAGCGCTT
CTCCACTCAGGCCAAGGCGCAGCGCAAACCCCTGACCACCTTTCTTATCGAAAATCAGATCCTCTCCAGCCGGGAGTTGG
CTGATTTTTGCGAGCAGGAGTATGGCGTTCCCCTGCTCGACCTCGATGCCTTCGAGCTGGGGGATATCCCCCCCAAGTAC
ATCAACCAGAAGCTGATCGAGAAGCACCACGCCCTACCCATCTATTTGCAGGGACACACCCTCTACATTGCCATGGCTGA
CCCGACCAATGTGGCGGCGCTGGAGGAGTTCGGCTTCAACTTCGGCCTGCATACCGAAGCCCTGCTGGTGGAAGAGGAGA
AGCTGGTGCGCGCCATCGGCAAGCTGCTTGAACACGATCAGGAGAACCTTAATGTCGATGATATCGATGACGCCGATATA
TCAGGCCTTGAGGTCGCCGATGAGCAGAGTCGTCTTGATGAAACGGTCAACAGCAGCGATGACGATGCTCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCTATTCGCCGCGGCGCATCGGATCTGCACTTCGAACCTTACGAACACAAGTACC
GCATTCGCTTTCGCATCGATGGCATTTTGCATGAGGCCGCCACCCCGCCGGTGAACTTGGCCAGCCGCTTCGCCGCGCGG
CTCAAGGTGATGGCCCGCCTCGATATCGCCGAGCGCAGGCTGCCGCAAGATGGCCGCATTAAGCTGAAACTCTCCCGCAA
CCGGGCCATGGACATGCGAGTCAACACCCTGCCCACCATGTGGGGCGAGAAGGTGGTGATCCGGCTGCTCGACTCATCGG
CAGCCCGCCTCAATATCGATCAGCTCGGCTTCGATGAGGAACAAAAGCAGCTCTACCAGACGGCCCTCGCCCGCCCCCAA
GGCATGATACTGGTGACCGGGCCCACAGGCTCCGGCAAGACGGTGACCCTCTACACAGGTTTGAATCTGCTCAACACGGT
GGAGCGCAACATCTCCACCGCCGAAGACCCCATCGAAATCAACCTGCCCGGCGTCAACCAGGTGCAGATCAATCCCAAGG
CGGGATTGACCTTCGCCGGCGCCCTGCGCGCCTTTTTGCGTCAGGATCCGGATGTGGTGATGGTGGGGGAGATCCGCGAT
CTGGAAACGGCTGAAATTGCCATCAAGGCTGCACAAACCGGCCATCTGGTGCTCTCAACCCTGCACACCAACTCCGCTGC
CGAGACCCTGACCCGCATGATGAACATGGGCGTTCCCGCCTTTAATATCGCTTCCTCGGTCACCCTTATCATGGCCCAGC
GACTGGCGCGCAAGCTGTGCGAACACTGCAAGCAACCCGACAACATCCCCGAAGGCGAGCTGCTCAAGCTTGGTTACAGC
GTGCAGCAAGTTGCGGCCGGCATCCGTCTCTATCGCCCCTGCGGATGCAAAGAGTGCTCCGGTGGCTATCGTGGGCGCAT
CGGTATTTACGAAATCCTCTCCATGTCAGACAACATCGCCCGCATCATCATGCAGGGTGGCAACTCACTGCTGATTGCAG
CCCAGGCACAAAAAGAGGGCATGCGCACGCTGCGCCAGGCCGGCCTCGAGAAGGCCCGCCTCGGTATCACCAGCCTGGCC
GAAATCAACCGGGTCACCACCAACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6WYY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.496

99.296

0.551
  pilB Acinetobacter baumannii D1279779

53.251

100

0.533
  pilB Acinetobacter baylyi ADP1

52.465

100

0.525
  pilB Vibrio cholerae strain A1552

52.867

98.239

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

50.883

99.648

0.507
  pilB Vibrio campbellii strain DS40M4

51.206

94.894

0.486
  pilF Neisseria gonorrhoeae MS11

45.922

99.296

0.456
  pilB/pilB1 Synechocystis sp. PCC 6803

42.376

88.908

0.377
  pilF Thermus thermophilus HB27

39.405

94.718

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.975

85.211

0.366

Multiple sequence alignment