Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FE254_RS02810 Genome accession   NZ_CP040349
Coordinates   625972..628089 (+) Length   705 a.a.
NCBI ID   WP_273252292.1    Uniprot ID   -
Organism   Pseudomonas guguanensis strain HMFL31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 620972..633089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE254_RS02790 (FE254_02830) pilM 623139..624203 (+) 1065 WP_273252300.1 pilus assembly protein PilM Machinery gene
  FE254_RS02795 (FE254_02835) pilN 624203..624775 (+) 573 WP_273252298.1 type 4a pilus biogenesis protein PilN -
  FE254_RS02800 (FE254_02840) pilO 624772..625392 (+) 621 WP_273252296.1 type 4a pilus biogenesis protein PilO -
  FE254_RS02805 (FE254_02845) pilP 625389..625916 (+) 528 WP_273252294.1 type 4a pilus biogenesis lipoprotein PilP -
  FE254_RS02810 (FE254_02850) pilQ 625972..628089 (+) 2118 WP_273252292.1 type IV pilus secretin PilQ Machinery gene
  FE254_RS02815 (FE254_02855) aroK 628095..628613 (+) 519 WP_090430282.1 shikimate kinase AroK -
  FE254_RS02820 (FE254_02860) aroB 628665..629768 (+) 1104 WP_273252290.1 3-dehydroquinate synthase -
  FE254_RS02825 (FE254_02865) - 629779..631395 (+) 1617 WP_273252288.1 AAA family ATPase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75924.10 Da        Isoelectric Point: 4.7604

>NTDB_id=363543 FE254_RS02810 WP_273252292.1 625972..628089(+) (pilQ) [Pseudomonas guguanensis strain HMFL31]
MNSSLSRLSVALLATLLSPAILAANLQDLSVASLPGDRVELKLSFDEPVTAPRGYTIEQPARIALDLPGVSNKLGSKNRE
LGVGNARSVTIVEAKDRTRLIVNLTSLAPYSTRVEGNDLYVLVGDSSAVAARPAASVPAPVASVPPKKTYGPQPKAISNI
DFQRGEQGEGNVVITLSDASVSPDIQEQGGKIRLDFAKTDLPESLRVRLDVKDFATPVQFVSATGSADKTSILVEPVGLY
DYLAYQTENTLTLSVKPLSQDDAERRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVAGNITLRLQNVP
WDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADMAKLFQSVTSADGAS
DTRGSVTVDERTNSIIAYQTQDRLDELRRIIAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGASRRGNWSVYGKDGAT
SFDSDSGQVFLPGTDTVGNVTLQDGVAPVPFVDMGVLGSTSGIGIGFLTDNVTLDLQLSAMEKTGNGEIVSQPKVVTSDK
ETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFARALNGVPPINKNEVNAKVLVN
DGETIVIGGVFSNTQTKGVEKVPFLGDLPFLGRVFRRDVVADNKAELLVFITPRIMNNQAIAVNR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=363543 FE254_RS02810 WP_273252292.1 625972..628089(+) (pilQ) [Pseudomonas guguanensis strain HMFL31]
ATGAACAGCTCTCTTTCGCGTCTGAGCGTCGCCTTGTTGGCTACGCTGCTGTCGCCCGCAATATTGGCGGCCAATCTGCA
GGATTTGAGTGTTGCCAGTCTGCCCGGGGATCGGGTGGAGCTGAAGCTTTCCTTCGATGAGCCGGTTACTGCTCCTCGTG
GCTATACCATCGAACAACCGGCGCGCATTGCGCTTGATTTGCCAGGGGTTTCCAACAAGCTTGGCTCGAAGAATCGTGAG
CTTGGTGTTGGCAATGCACGCAGCGTTACCATCGTCGAGGCCAAGGATCGCACCCGTCTGATCGTTAACCTCACCAGCCT
TGCCCCCTACAGCACTCGAGTCGAGGGCAATGACCTGTATGTATTGGTTGGCGACTCCTCTGCCGTTGCGGCGCGTCCGG
CGGCCTCTGTACCTGCTCCGGTAGCGTCGGTTCCGCCCAAGAAGACCTATGGTCCGCAACCGAAGGCCATCAGCAACATC
GACTTTCAGCGTGGCGAGCAGGGAGAGGGTAATGTCGTCATCACCTTGTCCGATGCTTCGGTAAGTCCAGATATTCAAGA
GCAAGGTGGCAAGATCCGTCTTGATTTCGCCAAGACGGATTTGCCCGAATCACTGCGCGTGCGTCTGGACGTGAAAGACT
TTGCGACGCCGGTGCAGTTCGTCAGTGCTACCGGCAGCGCCGATAAAACAAGCATCCTTGTCGAGCCGGTAGGTCTCTAT
GATTACCTGGCCTACCAGACTGAAAATACGCTGACACTGAGTGTCAAGCCGCTTTCTCAGGACGACGCCGAGCGTCGGAA
GGCTGAGCGTTTTGCCTACTCGGGCGAAAAGCTGTCGCTTAACTTCCAGGATATCGATGTACGATCGGTCCTGCAGCTGA
TTGCTGACTTTACCGATCTGAACCTGGTGGCGAGTGATACCGTTGCCGGCAATATCACCCTGCGTCTGCAGAACGTGCCT
TGGGACCAGGCCCTGGATTTGGTACTTAAGACCAAGGGTCTCGATAAGCGCCAAGTGGGTAACGTCCTGCTGGTCGCGCC
TGCTGACGAAATTGCCGCGCGTGAGCGGCAGGAGCTGGAGTCGCAGAAGCAGATTGCTGAGTTGGCGCCGCTGCGTCGGG
AACTGATCCAGGTGAATTACGCCAAGGCGGCAGACATGGCGAAACTGTTCCAGTCTGTCACCAGTGCTGACGGGGCCTCT
GATACTCGTGGCTCTGTAACCGTTGATGAGCGCACCAACAGCATCATTGCCTACCAGACGCAGGATCGGTTGGATGAGTT
GCGCCGTATCATTGCCCAGCTTGATATTCCTGTGCGTCAGGTGATGATCGAGGCGCGTATCGTCGAGGCCAATGTGGACT
ACGATAAAGCGCTTGGTGTTCGCTGGGGTGGCGCGAGTCGTCGCGGAAACTGGAGTGTCTACGGCAAGGATGGCGCAACC
TCCTTTGATAGCGATAGCGGGCAGGTGTTCCTGCCTGGCACTGATACCGTGGGCAACGTTACGCTGCAGGATGGTGTTGC
TCCGGTGCCTTTCGTTGATATGGGGGTGCTTGGCAGTACTTCGGGTATAGGTATCGGCTTCCTCACTGACAATGTCACGC
TGGATCTGCAGCTTTCCGCTATGGAGAAGACGGGTAATGGCGAGATCGTTTCTCAGCCGAAGGTTGTGACCTCGGACAAG
GAAACTGCCAAGATCCTGAAAGGACAGGAGGTTCCCTATCAGGAGGCAAGTTCCAGTGGGGCTACCAGTACTTCATTCAA
GGAGGCTGCCCTGTCTCTTGAGGTAACTCCGCAGATTACCCCGGACAATCGCATCATCATGGAGGTCAAGGTAACCAAGG
ACGCTCCAGACTTTGCGCGCGCTCTGAATGGTGTGCCGCCGATCAACAAGAACGAAGTCAACGCCAAGGTATTGGTCAAT
GATGGCGAGACGATCGTGATTGGTGGCGTATTCTCCAACACCCAGACCAAGGGTGTGGAGAAGGTCCCCTTCCTTGGCGA
TTTGCCATTCCTCGGTCGGGTGTTCCGCCGCGATGTCGTGGCAGACAACAAAGCCGAGTTGCTGGTTTTCATCACTCCGC
GCATCATGAACAACCAAGCCATTGCCGTGAATCGATGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

AMIN domain (AMIN)

(38-120)

Bacterial type II and III secretion system protein (Secretin)

(540-696)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

76.751

100

0.777
  pilQ Acinetobacter baumannii D1279779

39.891

100

0.417
  pilQ Acinetobacter baumannii strain A118

39.891

100

0.417
  comQ Acinetobacter baylyi ADP1

41.369

99.433

0.411
  pilQ Legionella pneumophila strain ERS1305867

37.518

96.028

0.36
  pilQ Legionella pneumophila strain Lp02

37.518

96.028

0.36

Multiple sequence alignment