Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FE254_RS02790 Genome accession   NZ_CP040349
Coordinates   623139..624203 (+) Length   354 a.a.
NCBI ID   WP_273252300.1    Uniprot ID   -
Organism   Pseudomonas guguanensis strain HMFL31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 618139..629203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FE254_RS02780 (FE254_02815) - 619115..620383 (+) 1269 WP_273252303.1 malic enzyme-like NAD(P)-binding protein -
  FE254_RS02785 (FE254_02825) - 620546..622957 (-) 2412 WP_273252302.1 penicillin-binding protein 1A -
  FE254_RS02790 (FE254_02830) pilM 623139..624203 (+) 1065 WP_273252300.1 pilus assembly protein PilM Machinery gene
  FE254_RS02795 (FE254_02835) pilN 624203..624775 (+) 573 WP_273252298.1 type 4a pilus biogenesis protein PilN -
  FE254_RS02800 (FE254_02840) pilO 624772..625392 (+) 621 WP_273252296.1 type 4a pilus biogenesis protein PilO -
  FE254_RS02805 (FE254_02845) pilP 625389..625916 (+) 528 WP_273252294.1 type 4a pilus biogenesis lipoprotein PilP -
  FE254_RS02810 (FE254_02850) pilQ 625972..628089 (+) 2118 WP_273252292.1 type IV pilus secretin PilQ Machinery gene
  FE254_RS02815 (FE254_02855) aroK 628095..628613 (+) 519 WP_090430282.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37843.22 Da        Isoelectric Point: 4.4647

>NTDB_id=363542 FE254_RS02790 WP_273252300.1 623139..624203(+) (pilM) [Pseudomonas guguanensis strain HMFL31]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEEDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYALLEAQLGGGHDDLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQYNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=363542 FE254_RS02790 WP_273252300.1 623139..624203(+) (pilM) [Pseudomonas guguanensis strain HMFL31]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCATACGCAGTCGAGCCGCTTCCGCCCAATGCAGTGGTCGAGAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCGCTCTCTCGCGTGTTGGCCAAGGCCAAGAGTGGCGTCAAGACGGCAGCCGTT
GCCGTTGCCGGGTCGGCTGTCATCACCAAGACCATCGAAATGGAAGCTGGGCTTTCCGAGGAAGATCTGGAAAACCAGCT
GAAGATCGAGGCTGACCAGTACATTCCCTATCCGCTGGAAGAAGTCGCCATCGATTTCGAGGTGCAGGGGCCGGCGCCGC
GCAACCCGGAGCGGGTCGAAGTGCTGCTGGCTGCATGCCGCAAGGAAAACGTCGAGGTTCGCGAGGCCGCGCTGGCGCTC
GCCGGTCTGACTGCCAAGGTGGTCGATGTCGAGGCCTATGCCCTGGAGCGTGCCTACGCTCTGCTGGAAGCGCAGCTGGG
TGGCGGGCACGACGATCTGACGGTTGCCGTGGTGGACATCGGTGCCACCATGACCACCCTCAGCGTTCTGCACAACGGCC
GCACCATCTATACCCGCGAGCAGCTCTTCGGCGGCAAGCAGCTGACCGAGGAGATCCAGAGGCGCTACGGGCTGTCGGTC
GAGGAAGCCGGTCTTGCCAAGAAGCAGGGTGGTCTTCCAGATGACTACGACAGCGAAGTGTTGCAACCGTTCAAGGAAGC
TGTCGTTCAGCAGGTGTCCCGTTCGCTGCAGTTCTTCTTCGCTGCCGGCCAATACAACGATGTCGACTATATCCTCCTGG
CTGGCGGTACCGCGTCCATCCCCGATCTTGATCGGCTGATCCAGCAGAAGATCGGCACCCAGACGCTCGTAGCCAATCCG
TTCGCCGATATGGCGCTCAGCAGCAAGGTCAACGCCGGCGCGTTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

45.198

100

0.452

Multiple sequence alignment