Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FAY22_RS13715 Genome accession   NZ_CP040128
Coordinates   2976127..2977815 (+) Length   562 a.a.
NCBI ID   WP_305778371.1    Uniprot ID   -
Organism   Noviherbaspirillum sp. UKPF54     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2971127..2982815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAY22_RS13685 (FAY22_13685) obgE 2971270..2972379 (-) 1110 WP_146330724.1 GTPase ObgE -
  FAY22_RS13690 (FAY22_13690) rpmA 2972472..2972729 (-) 258 WP_146330725.1 50S ribosomal protein L27 -
  FAY22_RS13695 (FAY22_13695) rplU 2972767..2973078 (-) 312 WP_040040654.1 50S ribosomal protein L21 -
  FAY22_RS13700 (FAY22_13700) ispB 2973376..2974305 (+) 930 WP_210411967.1 octaprenyl diphosphate synthase -
  FAY22_RS13710 (FAY22_13710) - 2974509..2975810 (+) 1302 WP_146330727.1 HlyC/CorC family transporter -
  FAY22_RS13715 (FAY22_13715) pilB 2976127..2977815 (+) 1689 WP_305778371.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FAY22_RS13720 (FAY22_13720) pilC 2977829..2979061 (+) 1233 WP_146330729.1 type II secretion system F family protein Machinery gene
  FAY22_RS13725 (FAY22_13725) pilD 2979061..2979927 (+) 867 WP_146330730.1 A24 family peptidase Machinery gene
  FAY22_RS13730 (FAY22_13730) coaE 2979929..2980576 (+) 648 WP_146330731.1 dephospho-CoA kinase -
  FAY22_RS13735 (FAY22_13735) zapD 2980697..2981452 (+) 756 WP_146330732.1 cell division protein ZapD -
  FAY22_RS13740 (FAY22_13740) yacG 2981464..2981646 (+) 183 WP_146330733.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61500.93 Da        Isoelectric Point: 4.9430

>NTDB_id=362400 FAY22_RS13715 WP_305778371.1 2976127..2977815(+) (pilB) [Noviherbaspirillum sp. UKPF54]
MSGLARALVQAGLLSAQQAETLHKKAHTEKSAFIDALIDSGTIEARSLASFCSETFGYPMLDFSAFNLSFLPEKIIDPKL
MQKARVIALGKRGNKVSVAISDPTNTHALDQIKFQTELTVEPIIVEHSALLKLVEKLGQSTEDSLDELVGDDLDIDLSEE
DMTAPQSEAVTADIDDAPVVKFLQKILIDAINMGASDLHFEPFEKFYRIRFRIDGVLREIAQPPIAIKEKLASRIKVISK
LDISEKRVPQDGRMKLVVSKNRSIDFRVSTLPTLFGEKIVMRILDATQAQMGIDALGYDPDQKEILLEAIQRPYGMVLVT
GPTGSGKTVSLYTCLNILNKPGINISTAEDPAEINLPGVNQVNVNDRAGLTFAVALKAFLRQDPDIIMVGEIRDLETADI
AIKAAQTGHMVFSTLHTNDAPSTLTRLMNMGVAPFNIASSVILITAQRLARRLCTCKQTTVIPDEALIEAGFKEEDLDGS
WLPYKAVGCERCSGSGYKGRVGIYQIMPITEDIERIILAHGTALEIEEQAKRDGVKTLREAGLVKVKQGLTSLEEVLGCT
NE

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=362400 FAY22_RS13715 WP_305778371.1 2976127..2977815(+) (pilB) [Noviherbaspirillum sp. UKPF54]
ATGTCCGGCCTTGCACGCGCGTTGGTGCAGGCTGGGCTGCTGTCCGCGCAGCAGGCGGAAACGCTGCACAAGAAGGCCCA
CACAGAAAAGAGTGCCTTTATCGATGCGTTGATCGACAGCGGTACTATAGAGGCACGTTCGCTGGCCAGCTTTTGCTCCG
AGACATTCGGTTATCCGATGCTCGATTTTTCGGCATTCAACTTGAGTTTCCTGCCCGAAAAGATCATCGATCCGAAGCTG
ATGCAAAAGGCGCGCGTCATTGCCTTGGGAAAGCGTGGCAACAAGGTTTCGGTCGCCATTTCCGATCCGACCAACACGCA
CGCGCTGGACCAGATCAAGTTTCAGACGGAACTGACCGTCGAGCCGATCATTGTCGAACACAGCGCCCTGCTGAAACTGG
TCGAGAAGCTCGGACAAAGCACTGAAGACAGCCTGGACGAACTGGTCGGCGACGATCTGGACATCGACCTCTCCGAAGAA
GACATGACCGCTCCGCAAAGCGAAGCGGTCACTGCCGATATCGATGACGCCCCGGTCGTCAAATTCCTGCAAAAGATCCT
GATCGACGCCATCAATATGGGGGCGTCGGACTTGCACTTCGAGCCGTTCGAGAAGTTCTACCGCATTCGCTTCCGTATCG
ACGGCGTGCTGCGCGAAATCGCCCAGCCCCCGATCGCCATCAAGGAAAAGCTGGCGTCGCGCATCAAGGTGATTTCCAAG
CTCGACATTTCGGAAAAACGGGTTCCGCAGGACGGCCGCATGAAGCTGGTCGTGTCGAAAAACCGGTCGATCGATTTTCG
CGTGAGCACCCTGCCGACGCTGTTTGGCGAAAAGATCGTGATGCGTATCCTAGATGCGACGCAGGCGCAGATGGGTATCG
ATGCGCTGGGCTACGATCCGGACCAGAAGGAAATCCTGCTGGAAGCGATTCAGCGGCCGTACGGCATGGTGCTGGTGACA
GGGCCGACGGGCTCGGGCAAGACGGTGTCGCTGTACACCTGCCTGAATATCCTTAACAAACCCGGCATCAACATCTCCAC
CGCAGAAGACCCAGCCGAAATCAACCTTCCCGGCGTAAACCAAGTCAACGTGAACGACCGTGCCGGGCTGACGTTCGCCG
TCGCGCTGAAGGCATTCCTGCGCCAGGATCCGGACATCATCATGGTGGGCGAAATCCGCGACCTGGAGACGGCGGACATC
GCCATCAAGGCCGCGCAGACAGGCCACATGGTGTTTTCGACACTGCACACCAACGACGCACCATCGACGCTGACACGCCT
GATGAACATGGGTGTGGCGCCGTTCAACATCGCGTCGTCGGTCATCCTGATTACCGCCCAACGGCTGGCGCGCCGTCTTT
GCACCTGCAAGCAAACCACCGTCATCCCCGACGAGGCACTGATCGAAGCCGGATTCAAGGAAGAAGACCTGGACGGCAGC
TGGCTGCCGTACAAGGCTGTCGGCTGCGAGCGCTGCAGCGGCAGCGGCTACAAAGGGCGTGTCGGCATTTATCAGATCAT
GCCCATCACGGAAGACATCGAGCGCATCATCCTGGCGCACGGCACGGCACTCGAAATCGAAGAGCAGGCCAAGCGCGACG
GGGTCAAGACGCTGCGCGAGGCCGGCCTGGTCAAGGTCAAGCAGGGATTGACGAGCCTGGAAGAGGTGCTTGGCTGCACC
AATGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(8-142)

Type II/IV secretion system protein (T2SSE)

(185-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.752

100

0.56
  pilB Acinetobacter baumannii D1279779

55.044

100

0.553
  pilF Neisseria gonorrhoeae MS11

54.707

100

0.548
  pilB Legionella pneumophila strain ERS1305867

51.064

100

0.512
  pilB Vibrio cholerae strain A1552

46.441

100

0.464
  pilB Vibrio parahaemolyticus RIMD 2210633

45.745

100

0.459
  pilB Vibrio campbellii strain DS40M4

44.583

100

0.447
  pilF Thermus thermophilus HB27

40.14

100

0.409
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.543

100

0.4

Multiple sequence alignment