Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   FAY22_RS13725 Genome accession   NZ_CP040128
Coordinates   2979061..2979927 (+) Length   288 a.a.
NCBI ID   WP_146330730.1    Uniprot ID   -
Organism   Noviherbaspirillum sp. UKPF54     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2974061..2984927
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAY22_RS13710 (FAY22_13710) - 2974509..2975810 (+) 1302 WP_146330727.1 HlyC/CorC family transporter -
  FAY22_RS13715 (FAY22_13715) pilB 2976127..2977815 (+) 1689 WP_305778371.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FAY22_RS13720 (FAY22_13720) pilC 2977829..2979061 (+) 1233 WP_146330729.1 type II secretion system F family protein Machinery gene
  FAY22_RS13725 (FAY22_13725) pilD 2979061..2979927 (+) 867 WP_146330730.1 A24 family peptidase Machinery gene
  FAY22_RS13730 (FAY22_13730) coaE 2979929..2980576 (+) 648 WP_146330731.1 dephospho-CoA kinase -
  FAY22_RS13735 (FAY22_13735) zapD 2980697..2981452 (+) 756 WP_146330732.1 cell division protein ZapD -
  FAY22_RS13740 (FAY22_13740) yacG 2981464..2981646 (+) 183 WP_146330733.1 DNA gyrase inhibitor YacG -
  FAY22_RS13745 (FAY22_13745) - 2981650..2983203 (-) 1554 WP_146330734.1 sigma-54-dependent transcriptional regulator -
  FAY22_RS13750 (FAY22_13750) - 2983353..2984648 (-) 1296 WP_146330735.1 D-amino acid dehydrogenase -

Sequence

Protein


Download         Length: 288 a.a.        Molecular weight: 31125.08 Da        Isoelectric Point: 7.2371

>NTDB_id=362402 FAY22_RS13725 WP_146330730.1 2979061..2979927(+) (pilD) [Noviherbaspirillum sp. UKPF54]
MLDLLLFAPPGAVLPTVVAAIFGLLIGSFLNVVIHRLPKMMQRESDNYVAQESGQPLPHTDKYNIMVPRSACPQCGHQIT
ALENIPILSYLFLRGKCAECKAPISARYPIVELLTGALSGFLVWRFGSGLAGLSTLAFAYLLIAMTFIDLDTQLLPDDLT
LPLLWCGLLVNLNSAFVPLNDAVIGAVAGYLSLWSIYWLFKLTTGKEGMGYGDFKLLAALGAWLGWKMLPVIILLSSLVG
ALVGLSLILFARHGRDTPIPFGPYLAAAGLIALLYGRSIVEAYLGFAI

Nucleotide


Download         Length: 867 bp        

>NTDB_id=362402 FAY22_RS13725 WP_146330730.1 2979061..2979927(+) (pilD) [Noviherbaspirillum sp. UKPF54]
ATGCTCGATCTGCTCCTGTTTGCTCCTCCCGGAGCCGTGCTGCCGACGGTGGTCGCGGCGATTTTCGGCCTGCTCATCGG
CAGCTTCCTCAATGTCGTGATCCATCGGCTGCCCAAGATGATGCAGCGCGAGTCCGATAATTATGTTGCGCAAGAAAGCG
GCCAGCCCTTGCCACATACCGACAAGTACAACATCATGGTGCCGCGCTCGGCCTGCCCGCAGTGCGGCCACCAGATCACG
GCGCTCGAAAATATCCCGATACTCAGCTATCTTTTCCTGCGCGGCAAATGTGCTGAATGCAAGGCCCCGATTTCGGCCCG
CTATCCGATCGTCGAACTTCTGACCGGAGCATTGTCCGGGTTCCTGGTCTGGCGCTTCGGCAGCGGCCTGGCGGGCTTGT
CCACGCTGGCGTTTGCGTATCTACTGATCGCAATGACCTTCATCGATCTCGATACGCAACTGCTGCCCGACGACCTGACC
CTGCCGCTGCTGTGGTGCGGCCTCCTGGTCAACCTGAACAGCGCCTTCGTCCCGCTGAATGACGCCGTCATCGGTGCCGT
CGCTGGCTACCTCAGCTTGTGGAGCATTTACTGGCTTTTCAAGCTAACGACAGGCAAGGAAGGCATGGGATATGGCGACT
TCAAATTGCTGGCCGCGCTGGGCGCCTGGCTCGGCTGGAAGATGCTGCCGGTAATCATCCTACTGTCTTCTCTGGTCGGA
GCACTGGTGGGCCTGTCGCTGATTCTTTTTGCTCGCCATGGGCGAGACACGCCAATCCCGTTCGGCCCCTATCTGGCGGC
CGCAGGCCTGATCGCCTTATTGTATGGCCGTTCAATCGTGGAAGCGTATCTCGGTTTTGCGATCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-126)

Type IV leader peptidase family (Peptidase_A24)

(137-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

57.934

94.097

0.545
  pilD Vibrio campbellii strain DS40M4

55.147

94.444

0.521
  pilD Neisseria gonorrhoeae MS11

49.813

92.708

0.462
  pilD Acinetobacter baumannii D1279779

49.077

94.097

0.462
  pilD Acinetobacter nosocomialis M2

49.077

94.097

0.462

Multiple sequence alignment