Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FDM99_RS04250 Genome accession   NZ_CP040080
Coordinates   865323..867035 (-) Length   570 a.a.
NCBI ID   WP_032026985.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain SP304     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 860323..872035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FDM99_RS04220 (FDM99_04220) - 860485..860811 (+) 327 WP_000099685.1 pyrimidine/purine nucleoside phosphorylase -
  FDM99_RS04225 (FDM99_04225) rlmB 860913..861662 (+) 750 WP_137344814.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  FDM99_RS04230 (FDM99_04230) - 861698..862612 (+) 915 WP_000075011.1 DMT family transporter -
  FDM99_RS04235 (FDM99_04235) coaE 862609..863205 (-) 597 WP_032026983.1 dephospho-CoA kinase -
  FDM99_RS18705 (FDM99_04240) pilD 863207..864067 (-) 861 WP_001152273.1 prepilin peptidase Machinery gene
  FDM99_RS04245 (FDM99_04245) pilC 864067..865293 (-) 1227 WP_000279216.1 type II secretion system F family protein Machinery gene
  FDM99_RS04250 (FDM99_04250) pilB 865323..867035 (-) 1713 WP_032026985.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FDM99_RS04255 (FDM99_04255) tpiA 867326..868120 (+) 795 WP_032026986.1 triose-phosphate isomerase -
  FDM99_RS04260 (FDM99_04260) secG 868133..868462 (+) 330 WP_000555570.1 preprotein translocase subunit SecG -
  FDM99_RS04280 (FDM99_04280) rimP 869104..869628 (+) 525 WP_000777730.1 ribosome maturation factor RimP -
  FDM99_RS04285 (FDM99_04285) nusA 869666..871151 (+) 1486 Protein_806 transcription termination factor NusA -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63453.86 Da        Isoelectric Point: 5.1596

>NTDB_id=361918 FDM99_RS04250 WP_032026985.1 865323..867035(-) (pilB) [Acinetobacter baumannii strain SP304]
MSALHTSPKFTGFFRRLVEEKHVSAATMQTALDAAKRAKQDTVAYLIEEVHLSPLLLAETISAEFAEPYFDLDVYDTSQI
PKDLVDQKLILKHRILPLIQRGQILYVATSNPSNIEAIDAIRFNSKLLVEPVIVEHHKLEKVLGQHFAEESSFDFNDEEF
DLDVNLDDPTAQEDEEEAPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFMEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFAAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKIPADIPKQSLLEMGF
TEQDLAHPDFRVFQPVGCPECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=361918 FDM99_RS04250 WP_032026985.1 865323..867035(-) (pilB) [Acinetobacter baumannii strain SP304]
ATGTCAGCATTACATACATCTCCAAAATTTACGGGGTTTTTTCGACGTTTAGTCGAAGAAAAGCATGTGTCGGCAGCGAC
CATGCAAACTGCATTAGATGCAGCGAAAAGAGCTAAGCAGGACACAGTCGCATATTTAATTGAAGAGGTTCATCTCTCCC
CTTTGTTATTGGCTGAAACAATTTCTGCTGAGTTTGCTGAACCATATTTCGATCTGGATGTTTATGACACCTCTCAGATT
CCTAAAGATCTGGTCGATCAGAAACTGATTTTAAAACATCGAATTTTGCCGCTCATTCAAAGAGGGCAAATTTTATATGT
TGCGACCAGTAATCCAAGCAATATCGAAGCGATTGATGCAATTCGTTTTAATAGTAAATTGCTGGTTGAGCCTGTAATTG
TGGAGCACCATAAACTCGAAAAAGTTTTAGGCCAGCATTTTGCAGAAGAAAGCAGCTTTGACTTCAATGATGAAGAGTTT
GATCTCGATGTTAATCTTGATGATCCTACCGCTCAAGAAGATGAAGAAGAAGCTCCTCAAGGCGATGAAGCCCCGATTGT
TAAATATATTAATAAGTTATTAATTGATGCCATTCGTATGGGTGCTTCAGACTTACATTTTGAACCGTACGAAAAATCCT
ATCGGGTGCGCTATCGTGTCGATGGTGTGTTACGTCAAATTGCCAATCCCCCTCTACAGCTTGCAAACCGCTTGGCATCA
CGCTTAAAAGTAATGTCTCAAATGGACATTTCTGAAAAACGCGTCCCACAAGATGGTCGTATCAAGCTCAAGCTGTCAAA
AACCAAAGCGATTGATTTTCGTGTGAACTCCCTCCCGACTTTGTTTGGTGAAAAGTTAGTTCTACGTATTCTTGACCCAT
CAAGCGCAATGCTAGGAATTGATGCACTAGGATATGAAGAAGATCAAAAAGCTTTGTTTATGGAGGCATTAGATAAACCT
CAAGGCATGCTTTTAATTACCGGACCAACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAATATTTTAAATAC
TGAAAGCTCCAATATTTCTACTGCCGAAGACCCGGTAGAAATTAATCTTGAAGGGATTAATCAAGTTAACGTAAACCCCA
AGGTAGGGCTTACTTTTGCGGCCGCACTTAAATCATTTTTACGACAAGACCCTGACATTATTATGGTGGGTGAGATTCGT
GACTTGGAAACAGCAGAAATTGCGATTAAGGCGGCTCAAACCGGCCACATGGTGATGTCAACACTACATACTAATAGTGC
TCCGGAAACATTGACCCGTCTACGCAATATGGGGGTTCCTTCTTTTAATATTGCAACATCAGTAAACCTAGTTATTGCAC
AGCGTTTAGCACGTCGTCTTTGTTCTCAATGCAAAATACCTGCTGATATTCCTAAACAAAGCTTATTAGAAATGGGCTTT
ACCGAACAAGATTTAGCACACCCAGATTTTCGGGTTTTTCAGCCCGTAGGTTGCCCTGAATGTCGTGAAGGCTATAAAGG
CCGTGTCGGTATTTATGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAACGCTTTAGAAA
TCGCAGCTGCTTCTGAAAAATTGGGGTTTAATAATCTACGTCGTTCAGGTTTAAAAAAGGTGATGCAAGGGGTGACCTCT
TTACAAGAAGTTAACCGTGTCACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

99.298

100

0.993
  pilB Acinetobacter baylyi ADP1

79.825

100

0.798
  pilB Legionella pneumophila strain ERS1305867

50.617

99.474

0.504
  pilF Neisseria gonorrhoeae MS11

50.356

98.596

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

52.408

91.053

0.477
  pilB Vibrio campbellii strain DS40M4

50.48

91.404

0.461
  pilB Vibrio cholerae strain A1552

50.289

91.053

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

35.179

100

0.379
  pilF Thermus thermophilus HB27

37.011

98.596

0.365

Multiple sequence alignment