Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FAH67_RS07860 Genome accession   NZ_CP039886
Coordinates   1530251..1531930 (+) Length   559 a.a.
NCBI ID   WP_115287640.1    Uniprot ID   -
Organism   Neisseria flavescens strain ATCC 13120     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1525251..1536930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAH67_RS07850 (FAH67_07850) - 1528024..1528782 (+) 759 WP_003680277.1 nucleotidyltransferase domain-containing protein -
  FAH67_RS07855 (FAH67_07855) - 1528797..1529858 (+) 1062 WP_003680278.1 nucleotidyltransferase domain-containing protein -
  FAH67_RS07860 (FAH67_07860) pilF 1530251..1531930 (+) 1680 WP_115287640.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FAH67_RS07865 (FAH67_07865) pilG 1532010..1533251 (+) 1242 WP_115287641.1 type II secretion system F family protein Machinery gene
  FAH67_RS07870 (FAH67_07870) pilD 1533253..1534140 (+) 888 WP_003682471.1 A24 family peptidase Machinery gene
  FAH67_RS07875 (FAH67_07875) coaE 1534140..1534760 (+) 621 WP_003682473.1 dephospho-CoA kinase -
  FAH67_RS07880 (FAH67_07880) yacG 1534757..1534939 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  FAH67_RS07885 (FAH67_07885) glyQ 1535021..1535920 (+) 900 WP_003682476.1 glycine--tRNA ligase subunit alpha -
  FAH67_RS07890 (FAH67_07890) lspA 1536071..1536577 (+) 507 WP_112890684.1 signal peptidase II -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62330.64 Da        Isoelectric Point: 5.9994

>NTDB_id=360906 FAH67_RS07860 WP_115287640.1 1530251..1531930(+) (pilF) [Neisseria flavescens strain ATCC 13120]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLHYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQNFQKIALASDLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEAMQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLTSRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVTLYTSLNILNTEDVNISTAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIIAQRLLRRLCPKCKTPMERPPEAALLKAGFTKEDLAKDWTM
YRAVGCDSCRGKGYKGRAGVYEVMPVSDEMQKVIMNNGTEIDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=360906 FAH67_RS07860 WP_115287640.1 1530251..1531930(+) (pilF) [Neisseria flavescens strain ATCC 13120]
ATGAGTGTCGGATTATTGCGCGTTTTGGTTCAAAGCCAAACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGGGAAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCCAAAGCTCTGGGAGAGTTGGTTGCTC
GCGTATTCAGCTACCCCCTTTTGGATTTGCACTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAACTTTCAAAAAATCGCTTTGGCTTCAGATTTAAGTGTCGACTTGGTAGTAGTTCCAGACGACCAGCTCAGCTCATTGC
TGGAATGGCTGGGCCAACGTTCGACCACTATTCTGAAAGAAATCAACGACGAACATGAAGCAATGCAGCAATCGCAATCG
CTTTATATCGACAATGAAGAGGCTGAAGACGGCCCTATCCCCCGTTTCATCCACAAAACCTTATCCGATGCCCTGAACGC
GGGCGCATCAGACATCCACTTTGAATTCTACGAGCAAATGGCGCGCGTTCGTTTCCGTGTAGACGGGCAATTGCGTGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTGACATCGCGTATCAAAGTGATGGCGCGTTTGGATATTTCTGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACGTTGCC
AACATTGTTTGGCGAAAAAGTGGTAATGCGTATCTTGAATTCGGATGCGGCTTCGCTGAATATCGATCAACTCGGTTTTG
AGCCGTTTCAAAAAGAGATGTTGCTTGAAGCCATTCACCGTCCGTACGGTATGGTGTTGGTTACCGGTCCGACAGGATCG
GGTAAAACGGTAACGCTTTATACCAGTCTGAATATCCTGAATACAGAAGACGTTAATATTTCTACCGCCGAAGACCCGGC
CGAGATTAATTTGCCGGGTATCAATCAGGTTAACGTCAACGATAAACAGGGCCTGACTTTTGCCGCCGCGCTAAAATCGT
TCCTGCGCCAAGACCCCGACATCATCATGGTGGGCGAGATTCGTGATTTGGAAACAGCCGATATTGCGATTAAAGCGGCA
CAGACAGGACATATGGTGTTTTCGACGCTGCATACTAATAATGCTCCGGCGACTTTGTCGCGTATGCTGAACATGGGTGT
CGCACCGTTCAATATCGCCAGTTCCGTCAGCCTGATAATCGCCCAACGTCTGTTGCGCCGTCTGTGTCCGAAGTGCAAAA
CGCCGATGGAGCGGCCTCCCGAAGCGGCCTTGCTTAAAGCGGGGTTTACCAAAGAAGACTTGGCTAAGGATTGGACGATG
TACCGTGCCGTTGGCTGCGACAGTTGCAGGGGCAAGGGGTACAAAGGCCGTGCCGGGGTATATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACGGAAATTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGCGCCGGCCTCATGAAAGTCATGCAAGGACTGACCTCGTTGGAAGAAGTGACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.996

100

0.78
  pilB Acinetobacter baylyi ADP1

50.352

100

0.512
  pilB Acinetobacter baumannii D1279779

50.619

100

0.512
  pilB Legionella pneumophila strain ERS1305867

47.527

100

0.481
  pilB Vibrio cholerae strain A1552

46.099

100

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Vibrio campbellii strain DS40M4

43.915

100

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

98.39

0.379
  pilF Thermus thermophilus HB27

37.989

96.064

0.365

Multiple sequence alignment