Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E8E00_RS11385 Genome accession   NZ_CP039516
Coordinates   2464223..2465905 (+) Length   560 a.a.
NCBI ID   WP_096630814.1    Uniprot ID   A0A2A4GLI0
Organism   Salinivibrio sp. YCSC6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2459223..2470905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8E00_RS11360 (E8E00_11360) ampD 2459580..2460134 (-) 555 WP_096630826.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E8E00_RS11365 (E8E00_11365) - 2460162..2461466 (-) 1305 WP_069586615.1 diguanylate cyclase domain-containing protein -
  E8E00_RS11370 (E8E00_11370) - 2461463..2462455 (-) 993 WP_136588386.1 ABC transporter substrate-binding protein -
  E8E00_RS11375 (E8E00_11375) nadC 2462618..2463526 (+) 909 WP_096630817.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E8E00_RS17345 (E8E00_11380) - 2463775..2464215 (+) 441 WP_069586613.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  E8E00_RS11385 (E8E00_11385) pilB 2464223..2465905 (+) 1683 WP_096630814.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E8E00_RS11390 (E8E00_11390) pilC 2465915..2467129 (+) 1215 WP_096630811.1 type II secretion system F family protein Machinery gene
  E8E00_RS11395 (E8E00_11395) pilD 2467194..2468063 (+) 870 WP_096630808.1 A24 family peptidase Machinery gene
  E8E00_RS11400 (E8E00_11400) coaE 2468063..2468677 (+) 615 WP_096630805.1 dephospho-CoA kinase -
  E8E00_RS11405 (E8E00_11405) zapD 2468701..2469441 (+) 741 WP_096630802.1 cell division protein ZapD -
  E8E00_RS11410 (E8E00_11410) yacG 2469446..2469652 (+) 207 WP_069586608.1 DNA gyrase inhibitor YacG -
  E8E00_RS11415 (E8E00_11415) mutT 2469683..2470078 (-) 396 WP_096630799.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61857.11 Da        Isoelectric Point: 5.7368

>NTDB_id=359444 E8E00_RS11385 WP_096630814.1 2464223..2465905(+) (pilB) [Salinivibrio sp. YCSC6]
MIPTAQLYQAGLLTDAQRDMLGIGEHTSLSLAHILDAGVDPTALANTLATLYHLPFAESHQYPFKDTCYALDRRDLIQRH
QVLPLAQQGQELLLGVSDPDNAEAVADFRFSTGLNVRLVVLSQPDLDAALRHLYGADIDGTGDGITISDQDLAALTVTDS
PQEDSAELQSDQAPISRYIQQILVDAVRKKASDVHFEPFEHHYQIRFRQDGLLQTYAHPPAGVSRRLSTRLKILAQLNIA
ERRLPQDGRIKLTLTADHTVDMRVSTLPTIWGEKVVVRLLDNGQQQLTLHDLGLTEWQRQHYQDALDRPQGMILFTGPTG
SGKTVSLYSGLKYLNQPERNIATVEDPVEIQLEGVNQVNVNTAIGLDFATALRAFLRQDPDVVMVGEIRDLDTAEIGIKA
AQTGHLVLSTLHTNSAAESITRLSHMGIAPYNLASSLTLVVAQRLVRRLCPMCKQPHPADDPHLQHLANLYPTLTPNTLY
QAHPHGCYQCYQGYQGRTGVYEVLPVTETIASAIYRNASAIELTQLATEEGMWRLAESGIAKLNQGITSIQELTRVIQLA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=359444 E8E00_RS11385 WP_096630814.1 2464223..2465905(+) (pilB) [Salinivibrio sp. YCSC6]
ATGATCCCAACCGCTCAGTTGTATCAAGCCGGTTTATTGACTGATGCCCAGCGCGATATGCTGGGCATTGGCGAACATAC
GTCGCTCAGTCTTGCGCATATACTCGATGCTGGGGTTGACCCCACCGCGCTCGCCAACACCCTCGCCACGCTGTACCATC
TTCCGTTCGCAGAGAGCCATCAATATCCGTTTAAAGACACCTGCTATGCCCTCGACCGACGCGATTTAATTCAGCGTCAC
CAAGTGTTACCGCTTGCTCAGCAAGGCCAAGAGCTATTACTGGGGGTCAGTGACCCAGATAACGCAGAAGCTGTCGCCGA
CTTTCGCTTTTCTACTGGGCTCAATGTCCGGTTAGTGGTGCTCAGTCAGCCAGATTTGGATGCTGCGTTGCGCCACTTAT
ATGGGGCAGATATCGATGGCACTGGCGATGGTATCACCATCAGTGATCAGGACTTGGCGGCCCTGACCGTCACCGATAGC
CCACAAGAGGACAGTGCCGAGCTACAAAGCGATCAGGCGCCCATCAGTCGTTACATTCAACAGATCTTGGTGGATGCGGT
GCGCAAAAAAGCCTCCGACGTGCATTTCGAGCCCTTTGAGCATCACTACCAAATTCGTTTTCGCCAAGACGGTTTATTAC
AAACCTATGCTCATCCTCCAGCAGGGGTCTCGCGGCGACTGTCGACCCGGCTCAAAATCTTAGCCCAACTCAATATTGCC
GAACGACGTTTGCCACAGGACGGGCGCATCAAGCTGACCCTCACTGCCGACCATACCGTCGATATGCGCGTCTCTACCCT
GCCAACCATTTGGGGCGAGAAAGTAGTAGTTCGTCTATTGGATAATGGCCAGCAGCAATTAACCTTGCATGATTTAGGCT
TAACCGAGTGGCAACGCCAGCACTATCAAGACGCATTGGATAGACCGCAAGGCATGATTTTGTTTACCGGCCCAACCGGC
AGCGGCAAAACCGTCTCCTTGTACAGTGGGTTAAAGTATCTCAATCAGCCCGAGCGTAATATTGCTACCGTCGAAGATCC
GGTTGAGATCCAGCTAGAAGGCGTCAACCAAGTCAACGTTAATACGGCCATTGGTTTAGATTTTGCCACCGCACTCCGCG
CATTTTTGCGCCAAGATCCCGATGTAGTCATGGTGGGGGAAATTCGCGATCTCGACACCGCTGAAATCGGGATCAAGGCC
GCGCAAACCGGTCATCTGGTGTTATCAACCCTGCATACCAATTCCGCCGCCGAAAGCATCACCCGTTTGTCCCATATGGG
CATTGCCCCCTACAACCTTGCCAGCTCTCTAACTTTGGTGGTGGCCCAACGTTTAGTCCGCCGTTTATGCCCGATGTGTA
AACAGCCGCACCCAGCTGATGATCCACACCTTCAGCACTTAGCTAACCTCTATCCGACGTTAACCCCAAACACCTTGTAC
CAAGCTCATCCTCACGGGTGCTATCAGTGTTATCAAGGCTATCAGGGTCGCACAGGGGTGTATGAGGTGCTTCCTGTCAC
CGAGACTATTGCCAGCGCCATTTACCGCAATGCCAGTGCCATCGAACTGACCCAACTGGCGACGGAGGAAGGGATGTGGC
GATTAGCCGAGTCCGGGATTGCCAAACTCAATCAAGGGATCACCAGCATTCAAGAGCTTACTCGCGTTATTCAGCTTGCC
TAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-136)

Type II/IV secretion system protein (T2SSE)

(181-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A4GLI0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

53.929

100

0.539
  pilB Vibrio campbellii strain DS40M4

53.417

99.286

0.53
  pilB Vibrio cholerae strain A1552

53.861

92.5

0.498
  pilB Legionella pneumophila strain ERS1305867

46.923

92.857

0.436
  pilB Acinetobacter baumannii D1279779

45.817

93.929

0.43
  pilB Acinetobacter baylyi ADP1

45.769

92.857

0.425
  pilF Neisseria gonorrhoeae MS11

42.095

93.75

0.395
  pilF Thermus thermophilus HB27

38.857

93.75

0.364

Multiple sequence alignment