Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   LLKF_RS09710 Genome accession   NC_013656
Coordinates   2009730..2010377 (-) Length   215 a.a.
NCBI ID   WP_012898342.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis KF147     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2004730..2015377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLKF_RS09685 (LLKF_1940) atpF 2004799..2005305 (-) 507 WP_010906128.1 F0F1 ATP synthase subunit B -
  LLKF_RS09690 (LLKF_1941) atpB 2005320..2006033 (-) 714 WP_004255255.1 F0F1 ATP synthase subunit A -
  LLKF_RS09695 (LLKF_1942) - 2006078..2006293 (-) 216 WP_004255250.1 F0F1 ATP synthase subunit C -
  LLKF_RS09700 (LLKF_1943) - 2006478..2007254 (-) 777 WP_012898340.1 alpha/beta hydrolase -
  LLKF_RS09705 (LLKF_1944) comEC 2007539..2009749 (-) 2211 WP_012898341.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LLKF_RS09710 (LLKF_1945) comEA 2009730..2010377 (-) 648 WP_012898342.1 ComEA family DNA-binding protein Machinery gene
  LLKF_RS09715 (LLKF_1946) - 2010437..2011798 (-) 1362 WP_012898343.1 ABC transporter permease -
  LLKF_RS09720 (LLKF_1947) - 2011795..2012727 (-) 933 WP_004255238.1 ABC transporter ATP-binding protein -
  LLKF_RS09725 (LLKF_1948) - 2012832..2013230 (-) 399 WP_004255236.1 hypothetical protein -
  LLKF_RS09730 (LLKF_1949) - 2013339..2013902 (-) 564 WP_012898344.1 GNAT family N-acetyltransferase -
  LLKF_RS09735 (LLKF_1950) - 2014075..2015253 (-) 1179 WP_012898345.1 SLC13 family permease -

Sequence

Protein


Download         Length: 215 a.a.        Molecular weight: 23619.79 Da        Isoelectric Point: 4.7531

>NTDB_id=35913 LLKF_RS09710 WP_012898342.1 2009730..2010377(-) (comEA) [Lactococcus lactis subsp. lactis KF147]
MDKILEKVKEYWKMIVLIVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISPDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSTTTEKININQAD
VTELQKLTGVGQKKAQDIIDFRTKNGDFISLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide


Download         Length: 648 bp        

>NTDB_id=35913 LLKF_RS09710 WP_012898342.1 2009730..2010377(-) (comEA) [Lactococcus lactis subsp. lactis KF147]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAATTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGCCAGATGAACGTCTAGTTGATCTAATTAAGGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAACAACCACTGAAAAAATAAATATCAATCAAGCAGAT
GTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATTGATTTTCGGACGAAAAATGGTGA
TTTCATATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

SLBB domain (SLBB)

(70-125)

Helix-hairpin-helix motif (HHH_3)

(151-212)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

74.312

100

0.753
  comEA/celA/cilE Streptococcus mitis SK321

43.981

100

0.442
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

42.661

100

0.433
  comEA/celA/cilE Streptococcus mitis NCTC 12261

40.826

100

0.414
  comEA/celA/cilE Streptococcus pneumoniae Rx1

40.639

100

0.414
  comEA/celA/cilE Streptococcus pneumoniae D39

40.639

100

0.414
  comEA/celA/cilE Streptococcus pneumoniae R6

40.639

100

0.414
  comEA Latilactobacillus sakei subsp. sakei 23K

35.683

100

0.377

Multiple sequence alignment