Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   E7Z57_RS05370 Genome accession   NZ_CP039339
Coordinates   1086082..1087809 (-) Length   575 a.a.
NCBI ID   WP_064048493.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain UW386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1081082..1092809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E7Z57_RS05340 (E7Z57_05340) - 1081705..1082166 (+) 462 WP_064048488.1 NUDIX domain-containing protein -
  E7Z57_RS05345 (E7Z57_05345) yacG 1082159..1082374 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  E7Z57_RS05350 (E7Z57_05350) zapD 1082385..1083143 (-) 759 WP_064048489.1 cell division protein ZapD -
  E7Z57_RS05355 (E7Z57_05355) coaE 1083261..1083875 (-) 615 WP_064048490.1 dephospho-CoA kinase -
  E7Z57_RS05360 (E7Z57_05360) - 1083888..1084769 (-) 882 WP_138928648.1 prepilin peptidase -
  E7Z57_RS05365 (E7Z57_05365) pilC 1084772..1086037 (-) 1266 WP_064048492.1 type II secretion system F family protein Machinery gene
  E7Z57_RS05370 (E7Z57_05370) pilF 1086082..1087809 (-) 1728 WP_064048493.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E7Z57_RS05375 (E7Z57_05375) - 1088057..1089334 (-) 1278 WP_138928649.1 HlyC/CorC family transporter -
  E7Z57_RS05385 (E7Z57_05385) ispB 1089622..1090590 (-) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  E7Z57_RS05390 (E7Z57_05390) rplU 1090869..1091180 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  E7Z57_RS05395 (E7Z57_05395) rpmA 1091218..1091478 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  E7Z57_RS05400 (E7Z57_05400) obgE 1091654..1092754 (+) 1101 WP_064048495.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63726.49 Da        Isoelectric Point: 7.1546

>NTDB_id=358107 E7Z57_RS05370 WP_064048493.1 1086082..1087809(-) (pilF) [Ralstonia pseudosolanacearum strain UW386]
MTLGLSLAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIEAVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETSQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKETVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPSPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=358107 E7Z57_RS05370 WP_064048493.1 1086082..1087809(-) (pilF) [Ralstonia pseudosolanacearum strain UW386]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCACCCAGCTAGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGACACCGTACCGCCCGTGCTGTCGGCCAACAAGCATTTC
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTGGCCACCTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGGCGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCGCCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCTCGCAGATCGAATACGACCCGGGC
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTCAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAACCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGACGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACACTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAACATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATTAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAAGCCGCGCAGACCGGGCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCATTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGAGCCCACTGCCCAAGGAAACGCTG
CTGGACGCGGGTTTCAAGGAAGCCGACCTGGACGGCTCGTGGCAACCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

53.493

94.609

0.506
  pilB Acinetobacter baumannii D1279779

51.237

98.435

0.504
  pilB Acinetobacter baylyi ADP1

50

97.391

0.487
  pilB Legionella pneumophila strain ERS1305867

47.518

98.087

0.466
  pilB Vibrio cholerae strain A1552

45

97.391

0.438
  pilB Vibrio campbellii strain DS40M4

44.891

95.304

0.428
  pilB Vibrio parahaemolyticus RIMD 2210633

44.834

94.261

0.423
  pilB/pilB1 Synechocystis sp. PCC 6803

35.821

100

0.376
  pilF Thermus thermophilus HB27

38.214

97.391

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.504

95.304

0.367

Multiple sequence alignment