Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E6P07_RS02730 Genome accession   NZ_CP039268
Coordinates   582556..584271 (-) Length   571 a.a.
NCBI ID   WP_153974192.1    Uniprot ID   -
Organism   Thermochromatium tepidum ATCC 43061     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 577556..589271
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E6P07_RS02710 (E6P07_02710) - 579285..579629 (-) 345 WP_153974188.1 hypothetical protein -
  E6P07_RS02715 (E6P07_02715) coaE 579746..580372 (-) 627 WP_153974189.1 dephospho-CoA kinase -
  E6P07_RS02720 (E6P07_02720) pilD 580369..581259 (-) 891 WP_153974190.1 A24 family peptidase Machinery gene
  E6P07_RS02725 (E6P07_02725) pilC 581285..582535 (-) 1251 WP_153974191.1 type II secretion system F family protein Machinery gene
  E6P07_RS02730 (E6P07_02730) pilB 582556..584271 (-) 1716 WP_153974192.1 type IV-A pilus assembly ATPase PilB Machinery gene
  E6P07_RS02735 (E6P07_02735) - 584297..585130 (-) 834 WP_211363158.1 NYN domain-containing protein -
  E6P07_RS02740 (E6P07_02740) acs 585359..587305 (-) 1947 WP_153974194.1 acetate--CoA ligase -
  E6P07_RS02745 (E6P07_02745) - 587451..588128 (-) 678 WP_153974195.1 exonuclease domain-containing protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63353.71 Da        Isoelectric Point: 5.4829

>NTDB_id=357730 E6P07_RS02730 WP_153974192.1 582556..584271(-) (pilB) [Thermochromatium tepidum ATCC 43061]
MAKEAVTLKPSGLAWRLVKDQFISEFQMQTAYQEARSKGRTFVQHLVEEKILDSRRIAVVAAQEFGVPLLDLDAFDLSAL
PLNLVDERLINKHRALPLMRRGNRLFLAVSDPTNEQALEEIRFNTGLTTEPILVEDAKLGAAIHQAIQAQDTSMVGDFAD
AELEKLEIEGGEEDDTENPDAAVDEAPIVRYVNKILVDAITSGASDIHFEPFEKFFRIRFRQDGLLREVANPPLNIANRL
TSRLKVMSRMNIAERRVPQDGRIKLKLSRNRDIDFRVNTLPTLYGEKIVLRILDSSAAQVGVDQLGFEPEQRKAFLKAIQ
KPYGMILVTGPTGSGKTVSLYTALNLLNRPEVNISTVEDPVEIQVPGINQVNLNPKSGLTFAAALRAFLRQDPDIIMVGE
IRDLETAEIAVKAAQTGHLVLSTLHTNDAPQTLTRLANMGVPPFNIASSVLLIMAQRLVRRLCPHCRQLLDLPRETLLEE
GFTDQEIDAGLSIYGPVGCDKCNKGYRGRVGIFQVMPVSEETSRLILEGGNAMQLAEQARREGISDLRTSGLRKVKAGIT
SLEEVNRVTTD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=357730 E6P07_RS02730 WP_153974192.1 582556..584271(-) (pilB) [Thermochromatium tepidum ATCC 43061]
ATGGCGAAGGAAGCGGTCACACTCAAACCCTCCGGCTTGGCCTGGCGACTCGTCAAGGATCAATTCATCTCCGAATTCCA
AATGCAGACGGCTTATCAAGAGGCCCGGAGCAAGGGACGGACCTTCGTGCAGCATCTGGTCGAGGAGAAGATCCTCGACA
GTCGCCGTATCGCCGTCGTGGCCGCGCAGGAGTTCGGTGTCCCGCTGCTCGATCTGGATGCCTTCGATCTCTCCGCGCTG
CCGCTCAACCTGGTCGACGAGCGGCTGATCAACAAGCATCGGGCACTGCCTCTGATGCGCCGCGGCAACCGGCTGTTTTT
GGCCGTCTCGGACCCCACCAACGAGCAGGCGCTCGAGGAGATCCGCTTCAACACGGGCTTGACCACAGAACCCATCCTGG
TTGAGGACGCCAAGCTCGGTGCGGCCATCCATCAGGCGATCCAGGCCCAGGATACGAGCATGGTCGGCGACTTCGCCGAC
GCCGAACTGGAAAAGCTCGAGATCGAGGGCGGCGAGGAGGATGACACCGAAAACCCAGACGCAGCCGTCGATGAGGCGCC
GATCGTGCGCTACGTCAATAAGATCCTGGTCGATGCGATCACGTCCGGGGCCTCGGACATCCATTTCGAGCCGTTCGAGA
AGTTTTTCCGCATCCGCTTTCGTCAGGACGGACTGCTGCGCGAGGTCGCCAATCCGCCGCTCAACATCGCCAATCGCCTC
ACCTCACGCCTCAAAGTCATGTCGCGGATGAACATCGCCGAGCGGCGCGTGCCCCAGGATGGACGTATCAAACTCAAGCT
CAGCCGCAATCGCGACATCGATTTTCGCGTCAACACCTTGCCCACGCTGTATGGCGAAAAGATCGTGTTGCGGATCCTCG
ACTCCAGTGCGGCCCAGGTCGGGGTCGATCAACTCGGCTTCGAGCCCGAGCAGCGCAAGGCCTTTCTCAAGGCGATCCAA
AAACCCTATGGCATGATCTTGGTCACGGGCCCGACCGGCTCGGGCAAGACCGTCTCGCTCTACACCGCCCTCAATCTGCT
CAACCGCCCCGAGGTCAACATCTCCACGGTCGAGGACCCGGTCGAGATCCAGGTACCCGGGATCAATCAGGTCAATCTCA
ACCCCAAGAGCGGCCTGACCTTTGCCGCCGCGTTACGCGCCTTTCTACGCCAGGATCCGGACATCATCATGGTCGGCGAG
ATCCGCGACCTGGAGACCGCCGAGATCGCCGTCAAGGCCGCCCAGACCGGACACCTGGTGCTCTCGACCCTGCATACCAA
CGACGCGCCCCAGACCCTGACCCGTCTGGCCAACATGGGTGTACCGCCCTTCAACATTGCCTCGTCCGTACTGCTCATCA
TGGCGCAGCGTCTGGTGCGGCGGCTGTGTCCGCACTGCCGCCAACTTCTGGATCTGCCGCGGGAGACCCTGCTTGAAGAG
GGCTTCACTGACCAGGAGATCGACGCCGGATTGAGCATCTATGGTCCGGTCGGGTGCGACAAGTGCAACAAGGGTTATCG
TGGACGTGTCGGTATCTTCCAGGTGATGCCGGTCTCGGAGGAAACCTCGCGTCTGATCCTGGAGGGCGGAAATGCCATGC
AGCTGGCCGAGCAGGCCCGACGCGAAGGCATCTCGGATCTGCGCACCTCCGGACTGCGCAAGGTCAAGGCCGGCATCACC
AGCCTAGAAGAAGTCAACCGGGTCACCACCGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.143

99.299

0.567
  pilB Legionella pneumophila strain ERS1305867

55.496

98.774

0.548
  pilB Acinetobacter baylyi ADP1

55.142

98.774

0.545
  pilF Neisseria gonorrhoeae MS11

49.206

99.299

0.489
  pilB Vibrio cholerae strain A1552

49.283

97.723

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

47.687

98.424

0.469
  pilB Vibrio campbellii strain DS40M4

48.905

95.972

0.469
  pilF Thermus thermophilus HB27

41.316

95.797

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.35

97.023

0.382

Multiple sequence alignment