Detailed information NTG0356837

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: E5093_RS07765
• Genome accession: NZ_CP039031
• Coordinates: 1651745..1652938 (-)
• Length: 397 a.a.
• NCBI ID: WP_045794791.1
• Uniprot ID: A0A6C0Y670
• Organism: Acinetobacter indicus strain yy_1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1646745..1657938

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5093_RS07745 (E5093_07805)	-	1648225..1648674 (+)	450	WP_016658087.1	MaoC family dehydratase	-
E5093_RS07750 (E5093_07810)	-	1648778..1649806 (+)	1029	WP_005178175.1	helix-turn-helix transcriptional regulator	-
E5093_RS07755 (E5093_07815)	gcvH	1649875..1650249 (+)	375	WP_005178176.1	glycine cleavage system protein GcvH	-
E5093_RS07760 (E5093_07820)	-	1650505..1651686 (+)	1182	WP_160241281.1	MFS transporter	-
E5093_RS07765 (E5093_07825)	pilU	1651745..1652938 (-)	1194	WP_045794791.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
E5093_RS07770 (E5093_07830)	-	1653192..1654247 (-)	1056	WP_104488899.1	3-deoxy-7-phosphoheptulonate synthase	-
E5093_RS07775 (E5093_07835)	hppD	1655123..1656181 (+)	1059	WP_168383124.1	4-hydroxyphenylpyruvate dioxygenase	-
E5093_RS07780 (E5093_07840)	-	1656333..1657715 (+)	1383	WP_177155670.1	amino acid permease	-

Sequence

Protein

• Length: 397 a.a.
• Molecular weight: 44672.05 Da
• Isoelectric Point: 6.2356

>NTDB_id=356837 E5093_RS07765 WP_045794791.1 1651745..1652938(-) (pilU) [Acinetobacter indicus strain yy_1]
MYSAELLEDARKFMFHMLSKVVEFGGSDLFITADFPPSIKHQGMMKALGQQALTAEKTKLFAYSLMNEKQRSEFETDLEC
NFAISVPEVSRFRVNVFQQQLHVGMVIRTISAEIPNFSQLKLPESLKHIIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDSHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTEDGKGRRAAVEIMLNTPLISELILKGQFHELKSIMGKS
RELGMQTFDQALFDLYNEGAISYEEAIRNADSMNELRLQIKLKSNRPSEASAAMSFSMLEEPKPEDEDTESAPQQSA

Nucleotide

• Length: 1194 bp

>NTDB_id=356837 E5093_RS07765 WP_045794791.1 1651745..1652938(-) (pilU) [Acinetobacter indicus strain yy_1]
ATGTACAGTGCAGAATTACTCGAAGATGCGCGTAAGTTTATGTTTCATATGCTCAGCAAAGTGGTTGAATTTGGGGGATC
AGATTTATTTATTACCGCGGATTTTCCACCGAGCATTAAACATCAGGGCATGATGAAAGCGCTGGGACAACAGGCGCTGA
CTGCAGAAAAAACCAAGCTGTTTGCCTACAGCCTCATGAATGAAAAACAGCGTAGCGAGTTTGAAACTGATCTGGAATGC
AACTTTGCCATTAGTGTGCCCGAGGTATCGCGTTTTCGGGTCAATGTGTTTCAACAGCAGCTGCATGTCGGGATGGTGAT
TCGAACCATTAGCGCGGAAATTCCAAACTTTAGCCAGCTGAAACTGCCGGAATCACTGAAGCATATTATTATGGAAAAGC
GCGGTCTGGTGCTGGTGGTGGGGGGAACCGGTTCAGGTAAATCGACCTCTTTGGCGGCGATGATTGATCATCGTAATGAA
AATTCAGCCGGGCATATTATTACCGTGGAAGACCCGGTGGAATATGTGCACAAGCATAAAAAGTCGATGATTACCCATCG
TGAAGTCGGGGTCGACAGCCATTCCTGGCACAATGCGCTGAAAAATACCTTACGTCAGGCACCGGATGTGATTTTAATTG
GCGAGATCCGCGACACCGAAACCATGGAACATGCGATTGCCTTTGCTGAAACCGGGCATTTATGTTTGGGCACCTTGCAT
GCCAATAACGCCAACCAGACGCTGGACCGGATTATCAACTTTTTCCCGGAAGAGCGCCGTAACCAGTTGCTGATGGATCT
GTCGTCCAATATGAAGGCCATTATTTCCCAGCGTCTGGTGCGTACCGAAGATGGTAAAGGCCGCCGTGCGGCAGTCGAGA
TTATGCTGAATACGCCGCTGATTTCTGAACTGATTTTAAAAGGCCAGTTCCATGAGTTAAAGTCGATTATGGGCAAATCG
CGTGAACTGGGCATGCAGACGTTTGATCAGGCTCTATTTGATTTGTATAACGAAGGCGCGATTTCCTATGAAGAAGCAAT
TCGTAATGCCGATTCGATGAATGAATTGCGCTTACAGATTAAATTGAAAAGTAACCGGCCAAGCGAAGCTTCGGCAGCTA
TGAGCTTTAGCATGCTGGAAGAGCCAAAACCGGAAGATGAAGATACGGAATCTGCTCCTCAACAGAGTGCTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (117-282)

3D structure

• AlphaFold DB: A0A6C0Y670

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.568	93.199	0.537
pilU	Acinetobacter baylyi ADP1	54.42	91.184	0.496
pilU	Vibrio cholerae strain A1552	52.663	85.139	0.448
pilT	Acinetobacter baumannii strain A118	43.939	83.123	0.365
pilT	Acinetobacter nosocomialis M2	43.939	83.123	0.365
pilT	Acinetobacter baumannii D1279779	43.939	83.123	0.365
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.814	84.131	0.36