Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E5Q53_RS08690 Genome accession   NZ_CP038817
Coordinates   1800045..1800512 (-) Length   155 a.a.
NCBI ID   WP_005707325.1    Uniprot ID   -
Organism   Haemophilus parahaemolyticus strain NEB129     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1795045..1805512
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5Q53_RS08665 (E5Q53_08660) yacG 1795932..1796117 (-) 186 WP_005707322.1 DNA gyrase inhibitor YacG -
  E5Q53_RS08670 (E5Q53_08665) coaE 1796105..1796731 (-) 627 WP_005707349.1 dephospho-CoA kinase -
  E5Q53_RS08675 (E5Q53_08670) - 1796752..1797396 (-) 645 WP_005707389.1 A24 family peptidase -
  E5Q53_RS08680 (E5Q53_08675) pilC 1797403..1798599 (-) 1197 WP_005707359.1 type II secretion system F family protein Machinery gene
  E5Q53_RS08685 (E5Q53_08680) pilB 1798592..1799977 (-) 1386 WP_005707356.1 GspE/PulE family protein Machinery gene
  E5Q53_RS08690 (E5Q53_08685) pilA 1800045..1800512 (-) 468 WP_005707325.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  E5Q53_RS08695 (E5Q53_08690) radA 1800704..1802083 (+) 1380 WP_005707288.1 DNA repair protein RadA Machinery gene
  E5Q53_RS08700 (E5Q53_08695) - 1802098..1802988 (+) 891 WP_039863409.1 RimK family alpha-L-glutamate ligase -
  E5Q53_RS08705 (E5Q53_08700) rlmD 1802990..1804324 (-) 1335 WP_039863408.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  E5Q53_RS08710 (E5Q53_08705) - 1804338..1804592 (-) 255 WP_005707339.1 Txe/YoeB family addiction module toxin -
  E5Q53_RS08715 (E5Q53_08710) yefM 1804589..1804840 (-) 252 WP_005707332.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 16351.75 Da        Isoelectric Point: 8.7927

>NTDB_id=355783 E5Q53_RS08690 WP_005707325.1 1800045..1800512(-) (pilA) [Haemophilus parahaemolyticus strain NEB129]
MKSLTKKPLFLKPTKAFTLIELMIVIAIIAILATIAIPSYTTYTQKAALSELLRASASYKSDVEVCIYNTGEVTNCSGGS
NGVQANKASSDETKYLKSVSVASGVITVAGKGNIDGFGYTMTPTFSSNTISWKTTCTGSDISLFPANFCSTSSTN

Nucleotide


Download         Length: 468 bp        

>NTDB_id=355783 E5Q53_RS08690 WP_005707325.1 1800045..1800512(-) (pilA) [Haemophilus parahaemolyticus strain NEB129]
ATGAAATCTTTAACAAAAAAGCCTCTTTTTCTTAAGCCTACAAAGGCATTTACCTTGATTGAATTGATGATTGTGATTGC
CATCATTGCCATTCTAGCGACCATTGCGATCCCTTCTTATACCACTTATACGCAAAAAGCAGCTCTCTCTGAGCTACTAA
GGGCTTCTGCTTCGTATAAATCCGATGTAGAGGTTTGTATTTACAACACTGGTGAGGTAACGAATTGTTCAGGCGGTTCA
AATGGTGTACAAGCAAACAAAGCCTCAAGTGACGAAACCAAATACTTGAAATCTGTTTCTGTTGCGAGCGGGGTAATTAC
GGTAGCAGGTAAAGGCAATATTGATGGATTTGGCTATACTATGACGCCAACCTTTAGCTCCAATACGATTAGTTGGAAAA
CGACTTGTACGGGTTCTGATATAAGTTTATTCCCTGCAAATTTCTGCTCTACAAGCTCAACAAACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(14-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

72.368

98.065

0.71
  pilA Haemophilus influenzae 86-028NP

56.667

96.774

0.548
  pilA Haemophilus influenzae Rd KW20

56.522

89.032

0.503
  comP Acinetobacter baylyi ADP1

42.361

92.903

0.394

Multiple sequence alignment