Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5E96_RS04835 Genome accession   NZ_CP038515
Coordinates   1016297..1018003 (+) Length   568 a.a.
NCBI ID   WP_017409440.1    Uniprot ID   -
Organism   Aeromonas sp. 1805     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1011297..1023003
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5E96_RS04810 (E5E96_04830) pdhR 1012458..1013222 (-) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  E5E96_RS04815 (E5E96_04835) ampD 1013498..1014070 (-) 573 WP_043160629.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E5E96_RS04820 (E5E96_04840) - 1014218..1014688 (+) 471 WP_024944772.1 TIGR02281 family clan AA aspartic protease -
  E5E96_RS04825 (E5E96_04845) nadC 1014694..1015551 (+) 858 WP_166464211.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E5E96_RS04830 (E5E96_04855) tapA 1015874..1016290 (+) 417 WP_042066959.1 type IVa pilus major pilin TapA -
  E5E96_RS04835 (E5E96_04860) pilB 1016297..1018003 (+) 1707 WP_017409440.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  E5E96_RS04840 (E5E96_04865) pilC 1018119..1019360 (+) 1242 WP_043126919.1 type II secretion system F family protein Machinery gene
  E5E96_RS04845 (E5E96_04870) pilD 1019383..1020255 (+) 873 WP_171281660.1 A24 family peptidase Machinery gene
  E5E96_RS04850 (E5E96_04875) coaE 1020275..1020889 (+) 615 WP_024945430.1 dephospho-CoA kinase -
  E5E96_RS04855 (E5E96_04880) zapD 1020927..1021649 (+) 723 WP_024945431.1 cell division protein ZapD -
  E5E96_RS04860 (E5E96_04885) yacG 1021659..1021853 (+) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  E5E96_RS04865 (E5E96_04890) mutT 1021914..1022327 (-) 414 WP_016352173.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62538.93 Da        Isoelectric Point: 5.4342

>NTDB_id=354950 E5E96_RS04835 WP_017409440.1 1016297..1018003(+) (pilB) [Aeromonas sp. 1805]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=354950 E5E96_RS04835 WP_017409440.1 1016297..1018003(+) (pilB) [Aeromonas sp. 1805]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCCCTGCTCAGTGAGTCCGACTCACAACGCTA
TCTGAGCCAAGCCAAGGCACAGCGCAAACCTTTTGTGACTTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CTGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTACCCATCTACACCCAAGGGCACACCCTCTATATCGCCATGTCAGA
CCCGACCAATGTGTCTGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGCTGGTCGAGGAGAACA
AACTCACCACAGCCATCGGCAAGCTGATGGAGAACGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATT
TCCGAGCTGGAAGTGTCGGATGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCCGACGACGACGCGCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGAATAGACGGTATTCTGCATGAGATAGCGACCCCACCGGTCAACCTGGCCAACCGCTTCTCCGCCCGC
CTCAAGGTTATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTTTGCTCGACTCCTCCG
CGGCACGCCTCAATATCGAGCAGCTCGGTTTTGACGATCGCCAGAAGGCGCAATACCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGACTGACCTTCGCGAGCGCCCTGCGCTCTTTCTTGCGCCAAGACCCGGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTGCACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGTAAATTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGTTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAGGAGTGTTCGGGTGGCTACAAGGGCCGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGCCTAGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Legionella pneumophila strain ERS1305867

54.064

99.648

0.539
  pilB Vibrio cholerae strain A1552

53.982

99.472

0.537
  pilB Vibrio campbellii strain DS40M4

51.943

99.648

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.454

99.296

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373

Multiple sequence alignment