Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   D11S_RS09940 Genome accession   NC_013416
Coordinates   1990644..1992053 (+) Length   469 a.a.
NCBI ID   WP_012821127.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans D11S-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1985644..1997053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D11S_RS09920 (D11S_2066) pnp 1986122..1988251 (-) 2130 WP_032997542.1 polyribonucleotide nucleotidyltransferase -
  D11S_RS09925 (D11S_2067) nanQ 1988545..1989015 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  D11S_RS09930 (D11S_2069) ampD 1989489..1990043 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  D11S_RS09935 (D11S_2070) pilA 1990165..1990617 (+) 453 WP_005586498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D11S_RS09940 (D11S_2071) pilB 1990644..1992053 (+) 1410 WP_012821127.1 GspE/PulE family protein Machinery gene
  D11S_RS09945 (D11S_2072) pilC 1992046..1993269 (+) 1224 WP_012821128.1 type II secretion system F family protein Machinery gene
  D11S_RS09950 (D11S_2073) - 1993266..1993955 (+) 690 WP_005547547.1 prepilin peptidase -
  D11S_RS09955 (D11S_2074) coaE 1994005..1994628 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  D11S_RS09960 (D11S_2075) yacG 1994618..1994830 (+) 213 WP_005547550.1 DNA gyrase inhibitor YacG -
  D11S_RS09965 (D11S_2076) - 1994830..1995102 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -
  D11S_RS09970 (D11S_2077) - 1995499..1996872 (+) 1374 WP_012821129.1 alanine/glycine:cation symporter family protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52530.82 Da        Isoelectric Point: 6.1345

>NTDB_id=35489 D11S_RS09940 WP_012821127.1 1990644..1992053(+) (pilB) [Aggregatibacter actinomycetemcomitans D11S-1]
MQNPMAQGYLIHSQNHGDFTIAPELWLKNQQQQTVLLRYLALPLAENGQTLWLGLDSFTNLSACEAFSFLTGKTIEPVLI
ESQTLKTALQDLSPRVEKVEENQPLFYSALSQEQPTQPYDEPIIRLLNDIFESATAQKASDIHLEPQADCLQTRFRIDGV
LQVQNTVPLSIASRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDTLDFRLSTLATQFGEKAVLRLQQNRPVQLAFGEL
GMTESQQQRFRRALSQPQGLILVTGPTGSGKSISLYTALQYLNTSEKHIMTAEDPIEIQLPGIIQSQVNHPIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQCHEIDGSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYKGRVGVYQFLQPNLQHPQGYETDFADLGQSALEKLKDGTTDLTEIQRVLGQTHD

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=35489 D11S_RS09940 WP_012821127.1 1990644..1992053(+) (pilB) [Aggregatibacter actinomycetemcomitans D11S-1]
ATGCAAAATCCGATGGCGCAAGGTTATTTGATTCACAGCCAAAATCACGGGGACTTTACCATTGCGCCCGAGCTATGGCT
TAAAAATCAGCAACAACAAACCGTCCTGTTGCGTTATCTCGCCCTGCCCTTAGCAGAAAACGGGCAGACCTTGTGGCTAG
GGTTAGACTCGTTCACTAACCTGAGCGCCTGTGAAGCCTTTTCTTTTCTCACCGGCAAAACCATTGAGCCGGTATTAATT
GAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTA
TTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCG
CGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTG
CTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATAT
TAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCA
CCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCGGTGAACTG
GGCATGACGGAATCACAGCAACAGCGTTTTCGTCGTGCCTTGAGCCAACCACAAGGCTTGATTTTAGTCACCGGTCCGAC
AGGCAGCGGCAAAAGTATTTCTTTATATACGGCGTTGCAGTACTTAAATACGTCTGAAAAACACATTATGACGGCGGAAG
ATCCCATTGAAATTCAGCTCCCAGGCATTATTCAAAGCCAAGTGAATCACCCTATCGGCTTGGATTTCAGCCGATTGCTT
CGCACCTTTTTACGTCAAGATCCGGACATTATTATGTTAGGTGAAATTCGTGATGAAGAAAGTGCCGCCATGGCATTACG
CGCCGCGCAAACGGGGCATTTGGTGCTTTCGACGCTACATACCAACGACGCCCCTTCCGCCATTTCCCGCCTGCAACAAC
TCGGCATTCAATGCCATGAAATCGACGGCAGTTTGTTGCTGGTGATCGCCCAACGTTTGGTGCGCAAACGCTGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACC
GAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCA
CAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

69.214

100

0.695
  pilB Haemophilus influenzae 86-028NP

70.652

98.081

0.693
  pilB Glaesserella parasuis strain SC1401

57.328

98.934

0.567
  pilB Vibrio cholerae strain A1552

40.53

100

0.424
  pilB Acinetobacter baylyi ADP1

39.078

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

40.041

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.752

100

0.409
  pilB Acinetobacter baumannii D1279779

38.855

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.331

100

0.401

Multiple sequence alignment