Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   E4T63_RS02095 Genome accession   NZ_CP038438
Coordinates   462212..464290 (+) Length   692 a.a.
NCBI ID   WP_135294791.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain LBUM677     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457212..469290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS02075 (E4T63_02075) pilM 459424..460488 (+) 1065 WP_096794691.1 pilus assembly protein PilM Machinery gene
  E4T63_RS02080 (E4T63_02080) - 460488..461054 (+) 567 WP_134785244.1 PilN domain-containing protein -
  E4T63_RS02085 (E4T63_02085) pilO 461051..461674 (+) 624 WP_098966813.1 type 4a pilus biogenesis protein PilO -
  E4T63_RS02090 (E4T63_02090) - 461671..462198 (+) 528 WP_098966815.1 pilus assembly protein PilP -
  E4T63_RS02095 (E4T63_02095) pilQ 462212..464290 (+) 2079 WP_135294791.1 type IV pilus secretin PilQ Machinery gene
  E4T63_RS02100 (E4T63_02100) aroK 464295..464813 (+) 519 WP_027611316.1 shikimate kinase AroK -
  E4T63_RS02105 (E4T63_02105) aroB 464965..466065 (+) 1101 WP_027611317.1 3-dehydroquinate synthase -
  E4T63_RS02110 (E4T63_02110) - 466076..467665 (+) 1590 WP_123585560.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75087.39 Da        Isoelectric Point: 5.5724

>NTDB_id=354276 E4T63_RS02095 WP_135294791.1 462212..464290(+) (pilQ) [Pseudomonas fluorescens strain LBUM677]
MNRIFSTLGFSLWIALLSPMVQAASLKTLDVAALPGDRIELKLSFDSPPPQPKGYTTESPARIALDLPGVASQLTSKTRD
LGSGNARTATVVEANDRTRLIISLTQLTSYTSRVEGNNLFVVVGQGAKPAAPRPAAVAPRPVAAPVPAKAYTPVAKTIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDRLRVRLDVKDFATPVQFVNAAATGDRAVITVEPSGT
FDYSTYQTDNKLTVSIRPMTVDDLQKRNADRNSYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
IDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=354276 E4T63_RS02095 WP_135294791.1 462212..464290(+) (pilQ) [Pseudomonas fluorescens strain LBUM677]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCACCGATGGTTCAGGCGGCCAGCCTCAA
GACGCTGGATGTCGCGGCGTTACCGGGTGATCGCATCGAGTTGAAGCTGTCGTTCGACAGCCCGCCACCGCAACCCAAGG
GCTACACCACCGAATCGCCGGCGCGAATCGCCCTGGATCTGCCGGGTGTCGCCAGCCAACTGACGAGCAAGACCCGCGAT
CTGGGCAGCGGTAACGCGCGAACCGCCACAGTGGTGGAAGCCAACGACCGCACGCGCCTGATCATCAGCCTGACGCAACT
GACGTCCTACACCTCGCGGGTCGAGGGCAACAATCTGTTCGTGGTGGTCGGGCAGGGCGCCAAGCCTGCTGCGCCACGGC
CAGCCGCTGTTGCGCCGCGACCGGTCGCGGCTCCAGTGCCGGCGAAGGCTTATACCCCCGTCGCCAAAACCATTCGTGGT
GTCGACTTCCAGCGCGGCACGGCGGGTGAGGGCAACGTGGTCATTGACCTTTCGGATCCGACCATCGCCCCGGACATCCA
GGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCCGACAGGCTGCGGGTACGCCTCGACGTCAAGG
ATTTCGCCACCCCGGTGCAGTTCGTCAACGCTGCCGCCACCGGTGATCGTGCGGTCATCACCGTCGAACCCAGCGGCACG
TTCGACTATTCCACCTATCAGACCGACAACAAACTGACCGTCAGCATTCGCCCGATGACGGTCGATGATCTGCAAAAACG
TAACGCCGATCGCAACAGCTACAGCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATCGACGTGCGCTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGATACCGTGCAGGGCGGGATCACCTTGCGTCTGCAAAACGTG
CCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAACGTGTTGCTGGTGGC
GCCGGCCGACGAAATCGCCGCCCGCGAACGCCAGGAACTGGAGTCACAGAAACAGATCGCCGAACTTGCGCCGCTGCGCC
GCGAGCTGCTGCAAGTGAATTACGCCAAGGCCGCGGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCGAAA
ATCGACGAGCGTGGCTCGATCACTGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
ACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATTGTCGAAGCCAACGTCG
ATTACGACAAGAGTCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACCTCGGGGGTCAGCAAT
GGCTCGTCGACCACCATCGGCACGCCGGGCAGTACCAGTACCAACTCGCCGTTCGTCGACATGGGCACGGTGGGTAATAC
CTCGGGGATCGGCATCGCCTTCATTACCGACAATGTGCTGCTCGATCTTGAGCTGACCGCCATGGAGAAGACCGGCAACG
GTGAGATCGTCTCGCAGCCCAAAGTGGTCACCTCCGACAAAGAGACGGCGAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAGGCGAGCTCCAGCGGTGCGACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCCGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.147

100

0.756
  comQ Acinetobacter baylyi ADP1

40.693

100

0.408
  pilQ Acinetobacter baumannii D1279779

37.534

100

0.405
  pilQ Acinetobacter baumannii strain A118

37.534

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

37.741

97.254

0.367
  pilQ Legionella pneumophila strain Lp02

37.741

97.254

0.367

Multiple sequence alignment