Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   E4T63_RS02075 Genome accession   NZ_CP038438
Coordinates   459424..460488 (+) Length   354 a.a.
NCBI ID   WP_096794691.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain LBUM677     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 454424..465488
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E4T63_RS02065 (E4T63_02065) - 455171..456439 (+) 1269 WP_003220889.1 malic enzyme-like NAD(P)-binding protein -
  E4T63_RS02070 (E4T63_02070) - 456760..459207 (-) 2448 WP_096794690.1 penicillin-binding protein 1A -
  E4T63_RS02075 (E4T63_02075) pilM 459424..460488 (+) 1065 WP_096794691.1 pilus assembly protein PilM Machinery gene
  E4T63_RS02080 (E4T63_02080) - 460488..461054 (+) 567 WP_134785244.1 PilN domain-containing protein -
  E4T63_RS02085 (E4T63_02085) pilO 461051..461674 (+) 624 WP_098966813.1 type 4a pilus biogenesis protein PilO -
  E4T63_RS02090 (E4T63_02090) - 461671..462198 (+) 528 WP_098966815.1 pilus assembly protein PilP -
  E4T63_RS02095 (E4T63_02095) pilQ 462212..464290 (+) 2079 WP_135294791.1 type IV pilus secretin PilQ Machinery gene
  E4T63_RS02100 (E4T63_02100) aroK 464295..464813 (+) 519 WP_027611316.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38104.68 Da        Isoelectric Point: 4.9026

>NTDB_id=354275 E4T63_RS02075 WP_096794691.1 459424..460488(+) (pilM) [Pseudomonas fluorescens strain LBUM677]
MLGLFKKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPPNAVVEKNIAELEGVGHALSRVLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=354275 E4T63_RS02075 WP_096794691.1 459424..460488(+) (pilM) [Pseudomonas fluorescens strain LBUM677]
GTGCTGGGACTCTTCAAGAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACTTCGGTGAAGCTGCTGGAGTT
GAGCCGTCAGGGCGATCGCTACCGGGTCGAGGCCTACGCGGTAGAGCCGCTGCCGCCCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAAGGCGTGGGCCATGCCTTGTCCCGGGTGCTGGTCAAGGCACGCACCGGGCTCAAGAGCGTGGCGGTG
GCGGTGGCCGGTTCTGCGGTGATCACCAAGATCATCGAGATGGACGCCGGACTGACCGATGATGAGCTGGAAAACCAGCT
GAAGATCGAAGCCGACCAATACATTCCCTATCCACTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTGTCGCCGC
GCAACCCGGAGCGCGTCAGCGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTGGCGCTG
GCGGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCCTATGCGCTGGAGCGCTCGTTCGGCCTGCTCGCTACTCAACTGGC
AGCCTCGCAGGAACGCCTGACCGTGGCGGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GCATCATCTATACCCGTGAGCAGCTGTTCGGTGGCAGGCAGCTGACTGAGGAGATCCAGCGCCGCTACGGCCTGACCGTC
GAGCAGGCCGGGCTGGCGAAAAAGCAGGGCGGTCTGCCGGACGACTATGTCAGCGAGGTATTGCAACCGTTCCGTGATGC
TCTGGTGCAGCAGGTCTCGCGCTCGTTGCAGTTCTTCTTTGCCTCCGGGCAGTACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACCGCCTCGGTGCCGGGGCTGGATCGGCTGATCGAACAGCGTCTGGGCACCCCGACCCAGGTCGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGAGCCCTGGCCAGTGACGCGCCGGCCCTGATGATTGCTTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.367

100

0.554
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486

Multiple sequence alignment