Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA/cilB/dalA   Type   Machinery gene
Locus tag   ES298_RS05930 Genome accession   NZ_CP038253
Coordinates   1196903..1197751 (-) Length   282 a.a.
NCBI ID   WP_000705306.1    Uniprot ID   A0A098Z7D2
Organism   Streptococcus pneumoniae strain TVO_1901929     
Function   ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology)   
DNA processing Competence regulation

Genomic Context

Location: 1191903..1202751
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES298_RS05915 (ES298_06230) - 1192255..1192614 (-) 360 WP_001237219.1 YbaN family protein -
  ES298_RS05920 (ES298_06235) topA 1192720..1194807 (-) 2088 WP_000179650.1 type I DNA topoisomerase -
  ES298_RS05925 (ES298_06240) - 1194975..1196018 (-) 1044 WP_001812957.1 TIGR00341 family protein -
  ES298_RS11200 - 1196597..1196719 (+) 123 WP_001818236.1 hypothetical protein -
  ES298_RS05930 (ES298_06245) dprA/cilB/dalA 1196903..1197751 (-) 849 WP_000705306.1 DNA-processing protein DprA Machinery gene
  ES298_RS05935 (ES298_06250) - 1197847..1198536 (-) 690 WP_000643963.1 sugar phosphate nucleotidyltransferase -
  ES298_RS05940 (ES298_06255) - 1198548..1199426 (-) 879 WP_000837618.1 DMT family transporter -
  ES298_RS05945 (ES298_06260) licA 1199416..1200285 (-) 870 WP_000411209.1 choline kinase LicA -
  ES298_RS05950 (ES298_06265) - 1200302..1201324 (-) 1023 WP_000609893.1 ribitol-5-phosphate dehydrogenase -
  ES298_RS05955 (ES298_06270) - 1201329..1202036 (-) 708 WP_000638500.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 282 a.a.        Molecular weight: 31062.72 Da        Isoelectric Point: 5.4047

>NTDB_id=352700 ES298_RS05930 WP_000705306.1 1196903..1197751(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901929]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDVLAEFEF

Nucleotide


Download         Length: 849 bp        

>NTDB_id=352700 ES298_RS05930 WP_000705306.1 1196903..1197751(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901929]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATATCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGGTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(75-271)

DNA processing protein A sterile alpha motif domain (SAM_DprA)

(3-64)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A098Z7D2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA/cilB/dalA Streptococcus pneumoniae Rx1

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae D39

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae R6

100

100

1
  dprA/cilB/dalA Streptococcus pneumoniae TIGR4

100

100

1
  dprA/cilB/dalA Streptococcus mitis NCTC 12261

98.582

100

0.986
  dprA/cilB/dalA Streptococcus mitis SK321

97.872

100

0.979
  dprA Streptococcus mutans UA159

63.214

99.291

0.628
  dprA Lactococcus lactis subsp. cremoris KW2

55.714

99.291

0.553
  dprA Staphylococcus aureus N315

37.809

100

0.379
  dprA Haemophilus influenzae Rd KW20

38.202

94.681

0.362

Multiple sequence alignment