Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   ES300_RS09515 Genome accession   NZ_CP038251
Coordinates   1860858..1861874 (-) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain TVO_1901944     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1855858..1866874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES300_RS10695 - 1855936..1856067 (-) 132 WP_000768904.1 hypothetical protein -
  ES300_RS09475 (ES300_09980) - 1856068..1857258 (-) 1191 WP_000167768.1 acetate kinase -
  ES300_RS09480 (ES300_09985) comYH 1857309..1858262 (-) 954 WP_000345086.1 class I SAM-dependent methyltransferase Machinery gene
  ES300_RS09485 (ES300_09990) - 1858323..1858917 (-) 595 Protein_1873 class I SAM-dependent methyltransferase -
  ES300_RS09490 (ES300_09995) comGG/cglG 1859020..1859466 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  ES300_RS09495 (ES300_10000) comGF/cglF 1859444..1859905 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  ES300_RS09500 (ES300_10005) comGE/cglE 1859868..1860170 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ES300_RS09505 (ES300_10010) comGD/cglD 1860133..1860537 (-) 405 WP_000588023.1 competence type IV pilus minor pilin ComGD Machinery gene
  ES300_RS09510 (ES300_10015) comGC/cglC 1860530..1860856 (-) 327 WP_000738621.1 comG operon protein ComGC Machinery gene
  ES300_RS09515 (ES300_10020) comGB/cglB 1860858..1861874 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  ES300_RS09520 (ES300_10025) comGA/cglA/cilD 1861822..1862763 (-) 942 WP_000249568.1 competence type IV pilus ATPase ComGA Machinery gene
  ES300_RS09525 (ES300_10030) - 1862839..1863204 (-) 366 WP_238123406.1 DUF1033 family protein -
  ES300_RS09530 (ES300_10035) - 1863355..1864413 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  ES300_RS09535 (ES300_10040) nagA 1864576..1865727 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=352578 ES300_RS09515 WP_074017570.1 1860858..1861874(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901944]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=352578 ES300_RS09515 WP_074017570.1 1860858..1861874(-) (comGB/cglB) [Streptococcus pneumoniae strain TVO_1901944]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGTACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment