Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   ES300_RS09495 Genome accession   NZ_CP038251
Coordinates   1859444..1859905 (-) Length   153 a.a.
NCBI ID   WP_000250523.1    Uniprot ID   A0A0B7M7T3
Organism   Streptococcus pneumoniae strain TVO_1901944     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1854444..1864905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES300_RS09465 (ES300_09970) - 1854749..1855573 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  ES300_RS09470 (ES300_09975) rnpA 1855548..1855919 (-) 372 WP_000739246.1 ribonuclease P protein component -
  ES300_RS10695 - 1855936..1856067 (-) 132 WP_000768904.1 hypothetical protein -
  ES300_RS09475 (ES300_09980) - 1856068..1857258 (-) 1191 WP_000167768.1 acetate kinase -
  ES300_RS09480 (ES300_09985) comYH 1857309..1858262 (-) 954 WP_000345086.1 class I SAM-dependent methyltransferase Machinery gene
  ES300_RS09485 (ES300_09990) - 1858323..1858917 (-) 595 Protein_1873 class I SAM-dependent methyltransferase -
  ES300_RS09490 (ES300_09995) comGG/cglG 1859020..1859466 (-) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  ES300_RS09495 (ES300_10000) comGF/cglF 1859444..1859905 (-) 462 WP_000250523.1 competence type IV pilus minor pilin ComGF Machinery gene
  ES300_RS09500 (ES300_10005) comGE/cglE 1859868..1860170 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  ES300_RS09505 (ES300_10010) comGD/cglD 1860133..1860537 (-) 405 WP_000588023.1 competence type IV pilus minor pilin ComGD Machinery gene
  ES300_RS09510 (ES300_10015) comGC/cglC 1860530..1860856 (-) 327 WP_000738621.1 comG operon protein ComGC Machinery gene
  ES300_RS09515 (ES300_10020) comGB/cglB 1860858..1861874 (-) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  ES300_RS09520 (ES300_10025) comGA/cglA/cilD 1861822..1862763 (-) 942 WP_000249568.1 competence type IV pilus ATPase ComGA Machinery gene
  ES300_RS09525 (ES300_10030) - 1862839..1863204 (-) 366 WP_238123406.1 DUF1033 family protein -
  ES300_RS09530 (ES300_10035) - 1863355..1864413 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17821.32 Da        Isoelectric Point: 6.4832

>NTDB_id=352574 ES300_RS09495 WP_000250523.1 1859444..1859905(-) (comGF/cglF) [Streptococcus pneumoniae strain TVO_1901944]
MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=352574 ES300_RS09495 WP_000250523.1 1859444..1859905(-) (comGF/cglF) [Streptococcus pneumoniae strain TVO_1901944]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGAT
TGTCATCAGTGGGGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT
CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M7T3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae D39

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae R6

94.771

100

0.948
  comGF/cglF Streptococcus pneumoniae TIGR4

94.771

100

0.948
  comGF/cglF Streptococcus mitis SK321

93.464

100

0.935
  comGF/cglF Streptococcus mitis NCTC 12261

92.157

100

0.922

Multiple sequence alignment