Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   E3Z29_RS04660 Genome accession   NZ_CP038207
Coordinates   1003501..1005579 (+) Length   692 a.a.
NCBI ID   WP_134785246.1    Uniprot ID   -
Organism   Pseudomonas sp. S150     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 998501..1010579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E3Z29_RS04640 (E3Z29_04640) pilM 1000713..1001777 (+) 1065 WP_096794691.1 pilus assembly protein PilM Machinery gene
  E3Z29_RS04645 (E3Z29_04645) - 1001777..1002343 (+) 567 WP_134785244.1 PilN domain-containing protein -
  E3Z29_RS04650 (E3Z29_04650) pilO 1002340..1002963 (+) 624 WP_098966813.1 type 4a pilus biogenesis protein PilO -
  E3Z29_RS04655 (E3Z29_04655) - 1002960..1003487 (+) 528 WP_134785245.1 pilus assembly protein PilP -
  E3Z29_RS04660 (E3Z29_04660) pilQ 1003501..1005579 (+) 2079 WP_134785246.1 type IV pilus secretin PilQ Machinery gene
  E3Z29_RS04665 (E3Z29_04665) aroK 1005584..1006102 (+) 519 WP_134785247.1 shikimate kinase AroK -
  E3Z29_RS04670 (E3Z29_04670) aroB 1006254..1007354 (+) 1101 WP_027611317.1 3-dehydroquinate synthase -
  E3Z29_RS04675 (E3Z29_04675) - 1007365..1008954 (+) 1590 WP_098966820.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 75087.35 Da        Isoelectric Point: 5.5724

>NTDB_id=352295 E3Z29_RS04660 WP_134785246.1 1003501..1005579(+) (pilQ) [Pseudomonas sp. S150]
MNRIFSTLGFSLWIALLSPMVQAASLKTLDVAALPGDRIELKLSFDSPPPQPRGYTTESPARIALDLPGVASQLTSKTRD
LGSGNARTATVVEANDRTRLIISLTQLTSYTSRVEGNNLFVVVGQGAKPAAPRPAAVAPRPVAAPAPAKAYTPVAKTIRG
VDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDRLRVRLDVKDFATPVQFVNAAATGDRAVITVEPSGT
FDYSTYQTDNKLTVSIRPMTVDDLQKRNADRNSYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAK
IDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSN
GSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=352295 E3Z29_RS04660 WP_134785246.1 1003501..1005579(+) (pilQ) [Pseudomonas sp. S150]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGCTTTCACCGATGGTTCAGGCGGCCAGCCTCAA
GACGCTGGATGTCGCGGCGTTACCGGGTGATCGCATCGAGTTGAAGCTGTCGTTCGACAGCCCGCCACCGCAACCCAGGG
GCTACACCACCGAATCGCCGGCGCGAATCGCCCTGGATCTGCCGGGTGTCGCCAGCCAACTGACGAGCAAGACCCGCGAT
CTGGGCAGCGGTAACGCGCGAACCGCCACAGTGGTGGAAGCCAACGACCGCACGCGCCTGATCATCAGCCTGACGCAACT
GACGTCCTACACCTCGCGGGTCGAGGGCAACAATCTGTTCGTAGTGGTCGGGCAGGGCGCCAAGCCTGCTGCGCCACGGC
CAGCCGCTGTTGCGCCACGACCGGTCGCGGCTCCAGCGCCGGCGAAGGCTTATACCCCCGTCGCCAAAACCATTCGTGGT
GTCGACTTCCAGCGCGGCACTGCGGGGGAGGGCAACGTGGTCATTGACCTTTCGGACCCGACCATCGCCCCGGACATCCA
GGAGCATGACGGCAAGATCATCCTGAGCTTTGCTCGCACGCAGTTGCCCGACAGGCTGCGGGTGCGCCTCGACGTCAAGG
ACTTCGCCACGCCGGTGCAGTTCGTCAACGCTGCCGCCACCGGTGATCGTGCGGTCATCACCGTCGAACCCAGCGGCACG
TTCGACTATTCCACCTACCAGACCGACAACAAACTGACCGTCAGCATTCGCCCGATGACGGTCGATGACCTGCAAAAGCG
CAACGCTGACCGCAACAGCTACAGCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATCGACGTGCGCTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGATACCGTGCAGGGCGGGATCACCTTGCGTCTGCAAAACGTG
CCGTGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGGCTGGATAAACGCAAGATCGGCAACGTGTTGCTGGTGGC
GCCGGCCGACGAAATCGCCGCCCGCGAACGCCAGGAACTGGAGTCACAGAAACAGATCGCCGAACTGGCGCCGCTGCGCC
GCGAGCTGCTGCAAGTGAATTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCGAAA
ATCGACGAGCGTGGCTCGATCACTGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGACGA
ACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCCAACGTCG
ATTACGACAAGAGTCTGGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACCTCGGGGGTCAGCAAT
GGCTCGTCGACCACCATCGGCACACCGGGCAGTACCAGTACCAACTCGCCGTTCGTCGACATGGGCACGGTGGGTAATAC
CTCGGGGATCGGCATCGCCTTCATTACCGACAATGTGCTGCTCGATCTTGAGCTGACCGCCATGGAGAAGACCGGCAACG
GTGAGATCGTCTCGCAGCCCAAAGTGGTCACCTCCGACAAAGAGACGGCGAAGATCCTCAAGGGCACCGAGATTCCGTAT
CAGGAGGCGAGCTCCAGCGGTGCGACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACGCCGCAGATCAC
CCCCGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGC
CGATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGGTGAACGACGGCGAGACCATCGTGATTGGGGGCGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.287

100

0.757
  comQ Acinetobacter baylyi ADP1

40.837

100

0.409
  pilQ Acinetobacter baumannii strain A118

37.534

100

0.405
  pilQ Acinetobacter baumannii D1279779

37.534

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

37.89

97.254

0.368
  pilQ Legionella pneumophila strain Lp02

37.89

97.254

0.368

Multiple sequence alignment